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LC Derivatizing Reagent

Major Types and Applications

In order to achieve high sensitivity and selectivity of the analyte in high performance liquid chromatography (HPLC), labeling is performed with a derivatizing reagent appropriate for the detection method.

Methods include the pre-column method, in which the analytes are labeled in advance, and the post-column method, in which the analytes are labeled with derivatizing reagents after HPLC separation.

Code No. Product Name Detection Derivatization Functional group
(L08379) 4-Amino-3-penten-2-one Fluorescence post-column Aldehyde
013-09991 N-(4-Anilinophenyl)maleimide ECD   SH group
017-12101 1-Anthroyl Cyanide Fluorescence pre-column OH group
531-20721 BCA Protein Assay Kit Visible post-column Proteins, Peptides
028-07781 Bis(2,4-dinitrophenyl)Oxalate (DNPO) Fluorescence pre-column Chemiluminescence (H2O2)
048-20101 Bis[2-(3,6-dioxaoctyloxycarbonyl)-4-nitrophenyl]Oxalate (DOPO) Fluorescence pre-column Chemiluminescence (H2O2)
204-09161 Bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)-phenyl]Oxalate (TDPO) Fluorescence pre-column Chemiluminescence (H2O2)
028-07421 Bis(2,4,6-trichlorophenyl)Oxalate(TCPO) Fluorescence pre-column Chemiluminescence (H2O2)
349-04021 4-Bromomethyl-7-methoxycoumarin (Br-Mmc) Fluorescence pre-column Carboxylic acid
048-18251 Dansyl Chloride Fluorescence pre-column Proteins, Peptides
042-18254
044-18253
347-05541 1,2-Diamino-4,5-methylenedioxybenzene Dihydrochloride (MDB) Fluorescence pre-column α-Keto acid
343-05543
049-02632 p-Dimethylaminobenzaldehyde Fluorescence pre-column Guanidine compound
043-02635
049-23671 6,7-Dimethoxy-1-methyl-2(1H)-quinoxalinone-3-propionohydrazide (DMEQ-Hydrazide) Fluorescence pre-column Carboxylic acid for fluorescent labeling
349-03661 Fluorescein Isothiocyanate Isomer I (FITC-I) Fluorescence pre-column Amino acid
343-03664
342-04751 4-Fluoro-7-nitrobenzofurazan (NBD-F) Fluorescence pre-column Amino acid
348-04753
341-05441 4-Fluoro-7-sulfamoylbenzofurazan (ABD-F) Fluorescence pre-column SH group
347-05443
342-05111 4-Fluoro-7-sulfobenzofurazan Ammonium Salt (SBD-F) Fluorescence pre-column
348-05113
077-02751 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate (GITC) UV pre-column Optical resolution of amino group
073-02753
148-05911 2-(2,3-Naphthalimino)ethyl Trifluoromethane- sulfonate (NE-OTf) Fluorescence, UV pre-column Carboxylic acid
166-11301 9,10-Phenanthrenequinone Fluorescence pre-column Guanidine compound
162-11303
161-09261 o-Phthalaldehyde Fluorescence post-column Alkaloids, Nitrogen compounds
167-09263
169-09262
165-09264
345-05221 Sulforhodamine 101 Acid Chloride Fluorescence pre-column Proteins, Amino acid

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