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LC Derivatizing Reagent

Major Types and Applications

In order to achieve high sensitivity and selectivity of the analyte in high performance liquid chromatography (HPLC), labeling is performed with a derivatizing reagent appropriate for the detection method.

Methods include the pre-column method, in which the analytes are labeled in advance, and the post-column method, in which the analytes are labeled with derivatizing reagents after HPLC separation.

Code No. Product Name Detection Derivatization Functional group
(L08379) 4-Amino-3-penten-2-one Fluorescence post-column Aldehyde
013-09991 N-(4-Anilinophenyl)maleimide ECD   SH group
017-12101 1-Anthroyl Cyanide Fluorescence pre-column OH group
531-20721 BCA Protein Assay Kit Visible post-column Proteins, Peptides
028-07781 Bis(2,4-dinitrophenyl)Oxalate (DNPO) Fluorescence pre-column Chemiluminescence (H2O2)
048-20101 Bis[2-(3,6-dioxaoctyloxycarbonyl)-4-nitrophenyl]Oxalate (DOPO) Fluorescence pre-column Chemiluminescence (H2O2)
204-09161 Bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)-phenyl]Oxalate (TDPO) Fluorescence pre-column Chemiluminescence (H2O2)
028-07421 Bis(2,4,6-trichlorophenyl)Oxalate(TCPO) Fluorescence pre-column Chemiluminescence (H2O2)
349-04021 4-Bromomethyl-7-methoxycoumarin (Br-Mmc) Fluorescence pre-column Carboxylic acid
048-18251 Dansyl Chloride Fluorescence pre-column Proteins, Peptides
042-18254
044-18253
347-05541 1,2-Diamino-4,5-methylenedioxybenzene Dihydrochloride (MDB) Fluorescence pre-column α-Keto acid
343-05543
049-02632 p-Dimethylaminobenzaldehyde Fluorescence pre-column Guanidine compound
043-02635
049-23671 6,7-Dimethoxy-1-methyl-2(1H)-quinoxalinone-3-propionohydrazide (DMEQ-Hydrazide) Fluorescence pre-column Carboxylic acid for fluorescent labeling
349-03661 Fluorescein Isothiocyanate Isomer I (FITC-I) Fluorescence pre-column Amino acid
343-03664
342-04751 4-Fluoro-7-nitrobenzofurazan (NBD-F) Fluorescence pre-column Amino acid
348-04753
341-05441 4-Fluoro-7-sulfamoylbenzofurazan (ABD-F) Fluorescence pre-column SH group
347-05443
342-05111 4-Fluoro-7-sulfobenzofurazan Ammonium Salt (SBD-F) Fluorescence pre-column
348-05113
077-02751 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate (GITC) UV pre-column Optical resolution of amino group
073-02753
148-05911 2-(2,3-Naphthalimino)ethyl Trifluoromethane- sulfonate (NE-OTf) Fluorescence, UV pre-column Carboxylic acid
166-11301 9,10-Phenanthrenequinone Fluorescence pre-column Guanidine compound
162-11303
161-09261 o-Phthalaldehyde Fluorescence post-column Alkaloids, Nitrogen compounds
167-09263
169-09262
165-09264
345-05221 Sulforhodamine 101 Acid Chloride Fluorescence pre-column Proteins, Amino acid

Product List

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N-(4-Anilinophenyl)maleimide Discontinued

for SH Groups Modification

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep below 25 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
013-09991
200mg
Discontinued
1-Anthroyl Cyanide

for Hydroxy Groups Fluorescent Labeling

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.
GHS :

Comparison
Product Number
Package Size
Price
Availability
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Distributor
017-12101
100mg
List Price
161.00 USD

In stock in Japan

Bis(2,4-dinitrophenyl) Oxalate

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
028-07781
1G
List Price
135.00 USD

Out of Stock

Bis(2,4,6-trichlorophenyl) Oxalate

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
028-07421
1G
List Price
73.00 USD

In stock in Japan

Dansyl Chloride

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
042-18254
1G
List Price
92.00 USD

In stock in Japan

Distributor
044-18253
5G
List Price
319.00 USD

In stock in Japan

Distributor
048-18251
500mg
List Price
71.00 USD

In stock in Japan

p-Dimethylaminobenzaldehyde

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C. (RT)
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
049-02632
25G
List Price
30.00 USD

In stock in Japan

Distributor
043-02635
500g
Discontinued
DMEQ-Hydrazide

for Carboxylic Acids Fluorescent Labeling

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C. (RT)

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
049-23671
100mg
List Price
172.00 USD

In stock in Japan

GITC [2,3,4,6-Tetra-O-acetyl-β-D-glucopyranosyl Isothiocyanate]

for Amino Groups Optical Resolution

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
073-02753
1G
List Price
407.00 USD

In stock in Japan

Distributor
077-02751
200mg
Discontinued
Phthalaldehyde

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
161-09261
1G
List Price
28.00 USD

In stock in Japan

Distributor
167-09263
5G
List Price
58.00 USD

In stock in Japan

Distributor
169-09262
25G
List Price
198.00 USD

In stock in Japan

Distributor
165-09264
100G
List Price
605.00 USD

In stock in Japan

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.