Nucleic Acids (DNA / RNA)
In molecular biology experiments, nucleic acids are used as positive control and substrates of molecular weight maker. Fujifilm Wako offers nucleic acids from NIPPON GENE that are useful for these purpose.
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Use of λ Phage DNA
Bacteriophage λ is a type of virus that infects E. coli and has long been used as a vector in genetic engineering. Restriction enzyme cleavage sites in the λ phage DNA have been described in detail (Figure 1), and homemade markers can be prepared by digestion with restriction enzymes.
Figure 1 Structure of the λ phage genome and restriction sites
Details of the number and location of restriction sites can be seen here.
References
- Sanger, F. et al.: J. Mol. Biol., 162(4), 729(1982).
Nucleotide sequence of bacteriophage lambda DNA
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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