Silica Gel Packing Agent
Silica gel has a strong physical strength and is the most widely used as a column packing agent for analysis and purification in chromatographies such as HPLC, medium and low pressure chromatography, and open column chromatography. FUJIFILM Wako provides various types of silica gel that can be used in a wide range of applications from analysis to large-scale purification and fractionation. A flow chart for selection and the particle size distribution table of the FUJIFILM Wako's silica gels are shown below.
How to Select Silica Gel
1. Shape
In general, spherical silica gel is expected to improve resolution and reproducibility compared with crushed silica gel (reasonable price).
FUJIFILM Wako employs crushed silica gel for Wakogel® series and spherical silica gel for Wakosil® series.
2. Chemical Modification
Chemical modification is to bind various functional groups to silanol groups on the surface of silica gel to change silica gel functions. C18 modified silica gel (ODS) is an octadecyl group-bound silica gel and is suitable for reverse-phase chromatography. NH2 modified silica gel is an aminopropyl group-bound silica gel and is suitable for normal-phase, hydrophilic interaction, and ion exchange chromatography. Unmodified silica gel is mainly used for normal-phase chromatography.
3. Particle Size
The larger the particle size, the more suitable for open columns, and the smaller the particle size, the more suitable for flash chromatography or HPLC.
4. Pore Size and Specific Surface Area
The smaller the pore size, the larger the specific surface area of silica gel and the higher resolution of small molecules. On the other hand, the larger the pore size, the higher the resolution of high molecules such as proteins. Suitable pore size must be selected so that sample molecules retain the silica gels well.
More Information
Flow Chart for Selection of Silica Gel
Particle Size Distribution Table
The smaller the particle size, the higher the resolution. However, the analysis time becomes longer due to slow flow rate. On the other hand, the larger the particle size, the flow speed becomes faster, and the behavior is suitable for open columns.
List of Physical Properties for each Silica Gel Grade
| Silica gel for general purpose (unmodified) | ||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
| Wakosil® 60 series | ||||||||
| Wakosil® 60, 38～63 μm | Spherical | 38～63 μm | 6 nm | - | 450～550 m2/g | 6.2～7.5 | Open column, low pressure, flash chromatography |
This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. Because of spherical silica gel, it is expected to improve resolution and reproducibility compared with crushed types. Product name: 60 = pore size Shape: Totally porous spherical silica gel with a pore size of 6 nm Use: Open column, low pressure, flash chromatography |
| Wakosil® 60, 64 µm～210 μm | 64～210 μm | - | Open column | |||||
| Wakogel® 60N series | ||||||||
| Wakogel® 60N, 38 ～100 µm | Crushed | 38～100 µm | 6 nm | 0.85 mL/g | 550 m2/g | 6.5～7.5 | Open column, low pressure, flash chromatography |
This series has a smaller pore size than Wakogel® C series. Therefore, it is suitable for large-scale fractionation and purification owing to its high retention performance. Product name: 60 = pore size Shape: Crushed silica gel with a pore size of 6 nm Use : Open column, low pressure, flash chromatography |
| Wakogel® 60N, 63 ～212 µm | 63～212 µm | Open column | ||||||
| Wakogel® 60N, 150 ～425 µm | 150～425 µm | Open column | ||||||
| Wakogel® C series | ||||||||
| Wakogel® C-100 | Crushed | 150～425 µm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5～7.0 | Open column | This series is the most common silica gel of FUJIFILM Wako. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, low pressure, flash chromatography |
| Wakogel® C-200 | 75～150 µm | 5.5～7.0 | Open column | |||||
| Wakogel® C-300 | 45～75 µm | 5.5～7.5 | Open column, low pressure, flash chromatography |
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| Wakogel® C series E (economy) type | ||||||||
| Wakogel® C-200E | Crushed | 75～150 µm | 7 nm | 0.8 mL/g | 450 m2/g | 5.5～7.5 | Open column | This series has a reasonable price by partially changing the manufacturing process, even though it has the same specifications, including particle size, as Wakogel® C series. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, low pressure, flash chromatography |
| Wakogel® C-300E | 45～75 µm | Open column, low pressure, flash chromatography |
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| Wakogel®C series HG (high grade) type | ||||||||
| Wakogel® C-300HG | Crushed | 40～60 µm | 7 nm | 0.8 mL/g | 450 m2/g | 6.0～7.5 | Open column, low pressure, flash chromatography |
This series is high-grade silica gel with a smaller particle size and a sharp particle size distribution compared with Wakogel® C series. Product name: The number after C- = mesh size which silica gel passes Shape: Totally porous spherical silica gel Use: Open column, medium and low pressure, flash chromatography |
| Wakogel® C-400HG | 20～40 µm | Medium and low pressure, flash chromatography |
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| Wakogel® C-500HG | 5～20 µm | 6.0～7.0 | Medium and low pressure, flash chromatography |
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| Wakogel® FC | ||||||||
| Wakogel® FC-40 | Crushed | 20～40 µm | 7 nm | 0.8 mL/g | 450 m2/g | - | Flash chromatography | Silica gel specialized for flash chromatography with a smaller particle size and a sharp particle size distribution. Product name: The number after FC- = particle size Shape: Crushed silica gel Use: Flash chromatography |
| Wakosil® HC-N | ||||||||
| Wakosil® HC-N | Spherical | 35～63 µm | 3 nm | 0.6 mL/g | 780 m2/g | 6.5～7.5 | Open column, medium pressure, flash chromatography |
This silica gel has larger specific surface area than that of Wakosil® C series. Because of its high retention performance, it has large sample load capacity and high resolution. Shape: Spherical silica gel Use: Open column, medium pressure, flash chromatography |
| Silica gel for HPLC columns used for normal-phase chromatography (unmodified) | ||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
| Wakosil® 5SIL series | ||||||||
| Wakosil® 5SIL | Spherical | 4.0～6.0 μm | 6 nm | - | 500 m²/g | 1.0～4.0 | Silicagel for HPLC columns used for normal-phase chromatography |
Silica gel for HPLC columns used for normal-phase chromatography. Product name: The number after 5SIL- = pore size Shape: Totally porous spherical silica gel Use: HPLC column packing agent used for normal-phase chromatography |
| Wakosil® 5SIL-120 | 12 nm | 300 m²/g | ||||||
| Silica gel for general purpose (NH2 modified) | |||||||||||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features | |||||||||
| Wakogel® 50NH2 | |||||||||||||||||
| Wakogel® 50NH2 | Crushed | 38～63 μm | 6.5 nm | 0.7 mL/g | 450 m2/g | 8.5～11.5 | Open column, medium pressure, flash chromatography |
Silica gel chemically modified with an aminopropyl group and is suitable for separation and purification in normal-phase, hydrophilic interaction, and ion exchange chromatography. It also has the features that it can fractionate sugars and suppress tailing of basic compounds. Shape: Crushed silica gel modified with an aminopropyl group Use: Open column, medium pressure, flash chromatography |
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| Wakosil® 50NH2 | |||||||||||||||||
| Wakosil® 50NH2 (HC) | Spherical | 35～63 μm | - | - | 900 m2/g | 8.5～11.5 | Open column, medium pressure, flash chromatography |
Silica gel chemically modified with an aminopropyl group and is suitable for separation and purification in normal-phase, hydrophilic interaction, and ion exchange chromatography. It also has the features that it can fractionation of sugars and suppress tailing of basic compounds. Because its specific surface area is larger than Wakogel® 50NH2, it has high retention performance and large sample loading capacity. Shape: Spherical silica gel modified with an aminopropyl group Use: Open column, medium pressure, flash chromatography |
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| Silica gel for general purpose (C18 modified) | ||||||||||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features | ||||||||
| Wakosil® C18 series | ||||||||||||||||
| Wakosil® 25C18 (15〜30 μm 70% up) | Spherical | 15～30 μm | 12 nm | 1 mL/g | 330 m2/g | - | Open column, medium pressure, flash chromatography |
Silica gel chemically modified with a C18 group (ODS). It is suitable for separation and purification in reverse-phase chromatography, and a wide range of eluents from organic solvents to aqueous solutions can be used. Product name: The number before C18 = the particle size Shape: Spherical silica gel modified with a C18 group (ODS) Use: Open column, medium pressure, flash chromatography |
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| Wakosil® 40C18 (30〜50 μm 70% up) | 30〜50 μm | |||||||||||||||
| Wakosil® 50C18 | 38～75 μm | 1.2 mL/g | 280 m2/g | |||||||||||||
| Wakosil® 100C18 | 63～212 μm | 300 m2/g | Open column | |||||||||||||
| Silica gel for HPLC columns used for reverse-phase chromatography (C18) | |||||||||||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features | |||||||||
| Wakosil® C18 series | |||||||||||||||||
| Wakosil® 5C18 | Spherical | 5 μm | 15 nm | 1.2 mL/g | 300 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase |
This is the most common C18 group-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Product name: The number before C18 = particle size, -200 = Pore size 200Å (20 nm) Shape: Spherical silica gel modified with C18 group (ODS) Use: HPLC columns packing agent for reverse-phase chromatography |
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| Wakosil® 10C18-200 | Spherical | 10 μm | 20 nm | 1 mL/g | 200 m²/g | ||||||||||||
| Wakosil® C18 RS series | |||||||||||||||||
| Wakosil®-II 3C18 RS | Spherical | 3.0～3.7 μm | 9.5～11.5 nm | 0.95～1.10 mL/g | 390～430 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase |
This series is the C18 group-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Because its specific surface area is larger than that of Wakosil® 5C18, this series has a high retention function and separability of highly polar compounds. Product name: The number before C18 = particle size Shape: Spherical silica gel modified with C18 group (ODS) Use: HPLC columns packing agent for reverse-phase chromatography |
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| Wakosil®-II 5C18 RS | Spherical | 4.2～4.7 μm | 11～13 nm | 1.15～1.30 mL/g | 390～430 m²/g | ||||||||||||
| Wakosil® C18 HG | |||||||||||||||||
| Wakosil®-II 3C18 HG | Spherical | 3.2～3.7 μm | 11～13 nm | 0.85～1.00 mL/g | 280～320 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase |
This series is the C18 group-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. This silica gel has the highest number of theoretical plates of the C18 series and is suitable for separating basic compounds and metal coordination compounds. Product name: The number before C18 = particle size Shape: Spherical silica gel modified with C18 group (ODS) Use: HPLC columns packing agent for reverse-phase chromatography |
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| For preparation of TLC plate | |||||||||||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features | |||||||||
| Wakogel® B series | |||||||||||||||||
| Wakogel® B-0 | Crushed | 45 μm | - | - | - | 6.5～7.5 | For preparation of TLC plate | Silica gel for preparation of plates for thin-layer chromatography (TLC). By following the predetermined procedure, TLC can be prepared on a glass plate. Product name: The number after B- = content of the binder F = single fluorescent indicator is added FM = 3 fluorescent indicators mixture is added Shape: Crushed silica gel Use: For preparation of TLC plate |
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| Wakogel® B-5F | |||||||||||||||||
| Wakogel® B-5 FM | |||||||||||||||||
| For analysis of dioxins | ||||||||
| Product Name | Shape | Size | Pore Size |
Pore Capacity |
Specific Area |
pH | Intended Use | Features |
| Wakogel® DX | ||||||||
| Wakogel® DX | Crushed | 75～150 μm | - | - | - | - | For analysis of dioxins | Silica gel which is guaranteed a low concentration of dioxins by testing ‘‘Suitability for dioxins determination”. Shape: Crushed silica gel Use: For analysis of dioxins |
| For analysis and separation/purification of petrochemical products | ||||||||
| Product Name | Shape | Size | Pore Size |
Pore Capacity |
Specific Area |
pH | Intended Use | Features |
| Wakogel® Q series | ||||||||
| Wakogel® Q-12 | Crushed | 75～600 µm | - | - | - | 4.0～6.0 | For analysis and separation/purification of petrochemical products |
Silica gel for analysis of petrochemical products specific to separation and purification of aromatic hydrocarbons and olefins. Product name: The number after Q- = corresponding Davison’s product grade Shape: Crushed silica gel Use: For analysis and separation/purification of petrochemical products |
| Wakogel® Q-22 | max. 75 µm | |||||||
| Wakogel® Q-23 | 75～150 µm | |||||||
| Wakogel® Q-50 | 75～250 µm | |||||||
| Wakogel® Q-63 | max. 45 µm | |||||||
| For analysis of PCB | ||||||||
| Product Name | Shape | Size | Pore Size |
Pore Capacity |
Specific Area |
pH | Intended Use | Features |
| Wakogel® S-1 | ||||||||
| Wakogel® S-1 | Crushed | 63～425 µm | - | - | - | 4.5～6.5 | For analysis of PCB | Silica gel specific to separate PCBs and organochlorine pesticides. Shape: Crushed silica gel Use: For analysis of PCBs and organochlorine pesticides |
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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