Wako Blog

We introduce a wide range of topics of research, technical reports, tips on experiments and so on.

Analytical Chemistry Technical Report

[Chromatography Q & A]: What Methods are Available for LC/MS Analysis of Highly Polarized Analytes?

This article was written for No. 42 (October 2006), of Analytical Circle.

For columns used in LC/MS, highly versatile reverse-phase octadecyl-silica gel (C18, ODS) is a mainstay packing agent. This ODS packing agent, however, has difficulty in separating a particular type o...

Analytical Chemistry Technical Report

[Technical Report] Analysis of Veterinary Drugs, Tiamulin and Robenidine, Using Wakopak® Wakosil-II 5SIL-AQ

This article was written by Ms. Kimiko Yoshida, FUJIFILM Wako Pure Chemical Corporation, for Vol. 74 No. 3 (July 2006), of Wako Junyaku Jiho.
The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

Widespread proliferation of liquid chromatography-mass spectrometry (LC/MS) analysis has made LC/MS-compatible solvents, reagents, and analytical columns commercially available. For the analytical col...

Analytical Chemistry

[Chromatography Q & A]: Change into Simple Analytical Condition Using Smaller Volume of Solvent; Change of Column Size from 4.6 mmφ to 3.0 mmφ

This article was written for No. 52 (March 2009), of Analytical Circle.

When the column size is changed from 4.6 to 3.0 mm in internal diameter (I.D.), and the flow rate is reduced by half, the solvent volume can be reduced. Although analysis time is not reduced, such cha...

Analytical Chemistry Technical Report

[Chromatography Q & A]: Connection Types of HPLC Column Wakopak®

This article was written for No. 71 (December 2013), of Analytical Circle

In the past, manufacturers of HPLC systems applied different specifications to connectors. In HPLC systems using stainless-steel pipes and connectors, the first column determined specifications of the...

Analytical Chemistry Technical Report

[Technical Report] Characteristics of C30 HPLC Column Wakopak Navi C30-5

This article was written by Mamoru Kubota, FUJIFILM Wako Pure Chemical Corporation, for Vol. 69, No. 3 (July 2001) of Wako Junyaku Jiho. The content of this article is from the time of publication. It is not the latest information due to new knowledge and changes in regulatory rules after original publication.

While, as a reverse-phase HPLC column packing, C18(ODS) modified silica gel with a broad range of applicability and excellent durability is still the focus of interest, silica gel bonding alkyl groups...

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