An affinity tag (epitope tag) is a short polypeptide or protein designed to be attached to a target protein. A target protein can be detected or purified by attaching the tag for the target protein and using the tag antibody.
The PA tag is an affinity tag system of Japanese origin involving a peptide tag (GVAMPGAEDDVV) derived from PLAG sequence in human podoplanin. The anti-PA tag antibody (NZ-1) with a high affinity and specificity to the PA tag enables one-step high purification of PA-tagged proteins. It can also be applied to Western blotting, flow cytometry, and immunocytochemical staining.
In addition, this anti-PA tag antibody recognizes the loop structure of PA tag and can be applied to purification/detection of membrane proteins.