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[New Product] ImmunoOneStep™ Western
Dual Effect, One Solution: Time-Saving Blocking and Antibody Reaction in One Step[New Product] ImmunoOneStep™ Western
ImmunoOneStep™ Western is a ready-to-use solution that combines blocking and primary/secondary antibody reactions into a single step in Western blotting, enabling exceptionally rapid and efficient blocking and antibody reactions. Compared with conventional skim milk-based methods, it significantly reduces the time required for blocking and antibody reactions.
Data
Compared with conventional Western blotting
(B) Western blotting using this product
| Lane No. | Total protein amount (ng) |
|---|---|
| 1 | 1 |
| 2 | 3 |
| 3 | 10 |
| Sample | ： EGFP-overexpressing HEK293 cell lysate |
| Gel | ： SuperSep™ Ace, 10-20%, 17well (Product No. 198-15041) |
| Primary antibody | ： Anti β-Actin, Monoclonal Antibody (Product No. 010-27841） |
| Secondary antibody | ： Anti-Mouse IgG HRP Conjugated |
Using this product, endogenous β-actin in HEK293 cells was successfully detected. The detection sensitivity was higher than that of conventional Western blotting.
Related Product
Fujifilm Wako offers products essential for western blotting, such as luminescent substrates of peroxidase (ImmunoStar™ Series), transfer buffers, stripping solution and primary antibodies.
Luminescent substrates of peroxidase -ImmunoStar™ Series-
ImmunoStar™ Series are chemiluminescence reagents for Western blotting for detection of peroxidase-conjugated antibodies. ImmunoStar™ LD is a highly sensitive luminescence reagent. It is suitable for detecting lowly Expressed proteins. ImmunoStar™ Zeta is a highly sensitive luminescence reagent with an emphasis on good balance of luminescence intensity, stability, and quantitative dynamic range.
| Product Name | ImmunoStar™ LD | ImmunoStar™ Zeta |
|---|---|---|
| Feature | High sensitivity type | Balanced type |
| Luminescence intensity | +++ 20 to 100 times higher luminescence intensity than ImmunoStar™ Zeta |
++ |
| Luminescence stability | + | +++ |
| Membrane | PVDF, Nitrocellulose | |
| Detection method | CCD imager, X-ray film | |
| Reprobing | Available | |
＞Comparison with Competitors' Products
Transfer Buffers
AquaBlot™ 10x High Efficiency Transfer Buffer is a 10x transfer buffer with higher transfer efficiency than the conventional Towbin (tris-glycine) buffers. Use it after 10-fold dilution with deionized water. Addition of methanol is not required during preparation.
Stripping Solution
Stripping Solution is a reagent for removing the antibody from the membrane after luminescence detection during Western blotting, in 10 minutes. As the membrane can be used again for an antibody reaction after removal, a single membrane can be used for more than one antibody reaction.
Primary antibodies
Fujifilm Wako also has a lineup of primary antibodies which can be used for Western blotting.
| Target | Product No. | Product Name | Package Size |
|---|---|---|---|
| Cancer Research | |||
| ATRX | 017-26751 | Anti ATRX, Monoclonal Antibody (AMab-6) | 100 μg |
| CD133 | 012-28881 | Anti CD133, Monoclonal Antibody (CMab-43) | 100 μg |
| CD44 | 015-28991 | Anti CD44s, Monoclonal Antibody (C44Mab-46) | 100 μg |
| 012-29001 | Anti CD44v3, Monoclonal Antibody (C44Mab-6) | 100 μg | |
| 019-29011 | Anti CD44v4, Monoclonal Antibody (C44Mab-108) | 100 μg | |
| 016-29021 | Anti CD44v5, Monoclonal Antibody (C44Mab-3) | 100 μg | |
| 013-29031 | Anti CD44v6, Monoclonal Antibody (C44Mab-9) | 100 μg | |
| 010-29041 | Anti CD44v7/v8, Monoclonal Antibody (C44Mab-34) | 100 μg | |
| 017-29051 | Anti CD44v8, Monoclonal Antibody (C44Mab-94) | 100 μg | |
| 014-29061 | Anti CD44v9, Monoclonal Antibody (C44Mab-1) | 100 μg | |
| 011-29071 | Anti CD44v10, Monoclonal Antibody (C44Mab-18) | 100 μg | |
| EGFR | 016-28781 | Anti Human EGFR, Monoclonal Antibody (EMab-134) | 100 μg |
| IDH | 018-24081 | Anti IDH1-R132H, Monoclonal Antibody (HMab-1) | 100 μg |
| 013-26851 | Anti IDH1-R132H, Monoclonal Antibody (HMab-2) | 100 μg | |
| 015-24091 | Anti IDH1-R132S, Monoclonal Antibody (SMab-1) | 100 μg | |
| 011-24071 | Anti IDH2, Monoclonal Antibody (RMab-22) | 100 μg | |
| 015-25691 | Anti Mutated IDH1/2, Monoclonal Antibody (MsMab-1) | 100 μg | |
| Podoplanin | 018-24101 | Anti Human Podoplanin, Monoclonal Antibody (NZ-1.2) | 100 μg |
| 017-27091 | Anti Dog Podoplanin, Monoclonal Antibody (PMab-38) | 100 μg | |
| 015-24111 | Anti Mouse Podoplanin, Monoclonal Antibody (PMab-1) | 100 μg | |
| TERT | 010-26861 | Anti TERT, Monoclonal Antibody (TMab-6) | 100 μg |
| Exosome Research | |||
| CD9 | 014-27763 | Anti CD9, Monoclonal Antibody (1K) | 100 μL |
| 019-27953 | Anti CD9, Rat Monoclonal Antibody (30B) , Biotin Conjugated | 100 μL | |
| 019-28173 | Anti CD9, Rat Monoclonal Antibody(77B) | 100 μL | |
| 017-28211 | Anti CD9, Rat Monoclonal Antibody(77B), Biotin-conjugated | 50 μL | |
| CD63 | 012-27063 | Anti CD63, Monoclonal Antibody (3-13) | 100 μL |
| CD81 | 011-27773 | Anti CD81, Monoclonal Antibody (17B1) | 100 μL |
| 011-28111 | Anti CD81, Rat Monoclonal Antibody (9B), Biotin-conjugated | 50 μL | |
| 010-28223 | Anti CD81, Rat Monoclonal Antibody(9B) | 100 μL | |
| Metabolism Research | |||
| Faecalibacterium duncaniae | 019-28911 | Anti Faecalibacterium, Monoclonal Antibody (FD-L4F6-18K2) | 50 μL |
| 016-28921 | Anti Faecalibacterium, Monoclonal Antibody (FD-L5B6-33K2) | 50 μL | |
| 012-28901 | Anti Faecalibacterium, Monoclonal Antibody (FD-S2D3-18K1) | 50 μL | |
| Phocaeicola vulgatus | 010-28941 | Anti Phocaeicola, Monoclonal Antibody (PV-L1A6-117K2) | 50 μL |
| 013-28931 | Anti Phocaeicola, Monoclonal Antibody (PV-L2B7-117K1) | 50 μL | |
| 017-28951 | Anti Phocaeicola, Monoclonal Antibody (PV-S10F7-14K1) | 50 μL | |
| Segatella copri | 011-28971 | Anti Segatella, Monoclonal Antibody (SC-L10B5-35K1) | 50 μL |
| 014-28961 | Anti Segatella, Monoclonal Antibody (SC-S10C3-49K1) | 50 μL | |
| Neuroscience | |||
| Amyloid β (Aβ) | 013-26873 | Anti Human Amyloid β, Monoclonal Antibody(BAN50) | 50 μL |
| 014-26923 | Anti Amyloid β40, Monoclonal Antibody(BA27) | 50 μL | |
| 010-26903 | Anti Amyloid β42(43), Monoclonal Antibody(BC05) | 50 μL | |
| CRMP2 | 014-24821 | Anti CRMP2, Monoclonal Antibody (9F) | 100 μL |
| GAP-43 | 010-25401 | Anti Phosphorylated GAP-43 S96, Monoclonal Antibody (18-10H-9H) | 100 μL |
| GFAP | 018-27283 | Anti GFAP, Monoclonal Antibody(MO389) | 50 μL |
| Iba1 | 011-27991 | Anti Iba1, Goat | 100 μL |
| 016-20001 | Anti Iba1, Rabbit (for Western Blotting) | 50 μg | |
| OMP | 019-22291 | Anti Olfactory Marker Protein, Goat | 100 μL |
| α-Synuclein | 015-25191 | Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) | 50 μL |
| Tau | 017-26893 | Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38) | 50 μL |
| 012-26963 | Anti Mouse Tau, Rat Monoclonal Antibody(RTM47) | 50 μL | |
| 015-26953 | Anti Human Tau, Rat Monoclonal Antibody(RTM49) | 50 μL | |
| 010-27123 | Anti Phosphorylated Tau S262, Rat Monoclonal Antibody(TIP1-35) | 50 μL | |
| 012-27683 | Anti Phosphorylated Tau S422, Monoclonal Antibody(AP422) | 50 μL | |
| 016-26601 | Anti Phosphorylated Tau T181, Rat Monoclonal Antibody (2E2-A6) | 50 μL | |
| 016-26581 | Anti 3R-Tau, Rat Monoclonal Antibody(2A1-1F4) | 50 μL | |
| 013-26591 | Anti 4R-Tau, Monoclonal Antibody(3E8-1A6) | 50 μL | |
| Epitope (Affinity) Tag | |||
| DYKDDDDK | 018-22381 | Anti DYKDDDDK tag, Monoclonal Antibody | 200 μg |
| 014-22383 | Anti DYKDDDDK tag, Monoclonal Antibody | 1 mg | |
| 015-22391 | Anti DYKDDDDK tag, Monoclonal Antibody, Peroxidase Conjugated | 200 μL | |
| GST | 013-21851 | Anti Glutathione S-transferase, Monoclonal Antibody | 200 μg |
| GFP | 012-22541 | Anti Green Fluorescent Protein, Monoclonal Antibody(mFX75) | 100 μL |
| HA | 014-21881 | Anti Hemagglutinin, Monoclonal Antibody | 200 μg |
| 011-21911 | Anti Hemagglutinin, Monoclonal Antibody, Peroxidase Conjugated | 100 μL | |
| His | 017-23211 | Anti 6×Histidine, Monoclonal Antibody(21-48) | 200 μg |
| 014-23221 | Anti 6×Histidine, Monoclonal Antibody(28-75) | 200 μg | |
| 011-23091 | Anti 6×Histidine, Monoclonal Antibody(9C11) | 200 μg | |
| 010-23181 | Anti 6×Histidine, Monoclonal Antibody(9C11), Peroxidase Conjugated | 100 μL | |
| 010-21861 | Anti 6×Histidine, Monoclonal Antibody(9F2) | 200 μg | |
| 013-23171 | Anti 6×Histidine, Monoclonal Antibody(9F2), Peroxidase Conjugated | 100 μL | |
| c-Myc | 017-21871 | Anti c-Myc, Monoclonal Antibody | 200 μg |
| 013-26513 | Anti c-Myc, Monoclonal Antibody (10D11) | 200 μg | |
| 014-21901 | Anti c-Myc, Monoclonal Antibody, Peroxidase Conjugated | 100 μL | |
| PA | 016-25861 | Anti PA tag, Rat Monoclonal Antibody | 200 μL |
| V5 | 011-23591 | Anti V5 tag, Monoclonal Antibody(6F5) | 200 μg |
| Loading Control | |||
| β-Actin | 010-27841 | Anti β-Actin, Monoclonal Antibodies | 100 μg |
| α-Tubulin | 017-25031 | Anti α-Tubulin, Monoclonal Antibody | 200 μg |
| 013-25033 | 1 mg | ||
| β-Tubulin | 014-25041 | Anti β-Tubulin, Monoclonal Antibody | 200 μg |
| 010-25043 | 1 mg | ||
| GAPDH | 016-25523 | Anti GAPDH, Monoclonal Antibodies | 200 μg |
| 014-25524 | 1 mg | ||
| 015-25473 | Anti GAPDH, Monoclonal Antibody, Peroxidase Conjugated | 200 μg | |