Anti CD81, Rat Monoclonal Antibody(9B)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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20uL
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100uL
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In stock in Japan |
Document
Product Information
| Cross Reactivity | Human [Note] This antibody doesn’t recognize the mouse and rat CD81. It shows a little reactivity with bovine CD81. |
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| Clone No. | 9B |
| Host | Rat |
| Subclass | IgG2b |
| Formulation | 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide. |
| Label | Unconjugated |
▼Evaluating the reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as “non-reactive”.
Application
| ELISA (detection) | 1:2,000 – 1:16,000 |
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| Western Blot | 1:1,000 – 1:8,000 * Please use this antibody under non-reducing condition |
| Flow Cytometry | 1:200 – 1:2,000 |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
Western Blot
Isolated exosomes derived from culture supernatant, human serum, and human heparin plasma were electrophoresed under non-reducing conditions. And Western Blot was performed using this antibody.
| Sample | Exosomes isolated from cell culture supernatant of COLO201 cells, human serum, and human heparin plasma. |
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| Primary antibody | Anti CD81, Rat Monoclonal Antibody (9B) [code No. 010-28223] (1 μg/mL) |
| Secondary antibody | Anti Rat IgG (H+L), Donkey, Peroxidase-conjugated [code No. 564-73961] (1:20,000) |
| Detection reagent | ImmunoStar® Zeta [code No. 291-72401] |
| CD81 protein (26 kDa) in each samples (non-reducing condition) was detected with this product. |
Data of cross-reactivity
The cross-reactivity of this product with human, mouse, rat, and bovine serum was detected.
| Sample | Human serum, Mouse serum, Rat serum, and Bovine serum |
| ELISA Kit | PS Capture™ Exosome ELISA Kit (Streptavidin HRP) [code No. 298-80601] |
| Detection antibody | Anti CD81, Rat Monoclonal Antibody (9B) [code No. 010-28223] –biotin labeled (250 ng/mL) |
| The signal to human serum was set to 100%. No signals for mouse and rat serum were obtained, and bovine serum responded slightly. Although this antibody responds very slightly to bovine serum, it has been shown to be highly specific for human serum. |
Reactivity to FBS in culture medium
The reactivity of the antibody with FBS in the culture medium was confirmed.
| Sample | A: The culture medium containing 15% FBS B: Culture supernatant of cells cultured in a culture medium containing 15% FBS After A and B were concentrated 10 times, then the exosomes were isolated with our kit [MagCapture™ Exosome Isolation Kit PS [code No. 293-77601]]. The obtained recovered samples were electrophoresed under non-reducing conditions. |
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| Primary antibody | Competitor: Anti-CD81 antibody (competitor) (1 μg/mL) This product: Anti CD81, Rat Monoclonal Antibody (9B) [code No. 010-28223] (1 μg/mL) |
| Secondary antibody | HRP-conjugated Anti Mouse / Rat IgG antibody |
| Detection reagent | ImmunoStar® Zeta [code No. 291-72401] |
| The competitor’s antibody, which does not guranteed specificity to cross-reactivity was detected the exosomes from both A and B. This product, which has been confirmed to have low cross-reactivity with bovine CD81 did not detect the exosomes from A. And it detected the exosomes from B. *The reason for increasing the exposure time is to confirm that this antibody does not detect bovine CD81 from FBS. It was shown that the background caused by FBS added to the culture medium was not detected by using this antibody. |
Overview / Applications
| Outline | CD81 is 26 kDa cell surface protein and is known as TAPA-1 (Target of the Antiproliferative Antibody 1) and Tetraspanin-28 (Tspan-28). CD81 interacts directly with immunoglobulin superfamily member 8 (IGSF8, CD316) and CD36. It also forms a signal transduction complex with CD19, CD21 and Leu-13 (CD225) on the surface of B cell. In addition, on T cells CD81 associates with CD4 and CD8 and provides a costimulatory signal with CD3. This antibody is established against human CD81 with Rat. It specifically recognize human CD81 and very slightly bovine CD81. It does not recognize mouse and rat CD81. [Clone No.] 9B [Immunized animal] Rat [Antibody subclass] IgG2b |
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| Application | Western Blot 1:1000 - 1:8000 *Use this antibody under non-reducing condition. ELISA (detection) 1:2000 - 1:16000 Flow Cytometry 1:200 - 1:2000 Please optimize the most appropriate concentration for your analysis and samples. |
Property
| Specificity | Human CD81. Note: It shows a little reactivity with bovine CD81. It does not react with mouse and rat CD81. [Reactivity confirmation condition] The reactivity of this antibody against the serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as "not react". |
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| Composition | 1xTBS, 50w/v% glycerol and 0.05% sodium azide. |
| Concentration | antibody concentration : Indicated on the label. |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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