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Anti CD9, Rat Monoclonal Antibody (30B) , Biotin Conjugated

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
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Product Number
Package Size
Price
Inventory
Distributor
013-27951
Barcode No
4548995075857
20uL
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Distributor
019-27953
Barcode No
4548995075864
100uL
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In stock in Japan

Document

Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Information

Cross Reactivity Human
[Note] It shows a little reactivity with rat CD9.
Clone No. 30B
Host Rat
Subclass IgG2b
Formulation 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2
Label Biotin

▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".

Application

ELISA 1: 2,000 - 1: 8,000
Western Blot 1: 1,000 - 1: 4,000 *Please use this antibody under non-reducing condition

Please optimize the most appropriate concentration for your analysis and samples.

ELISA

Directly Detection of exosomes in biofluids

Exosomes in biofluids were detected using PS Capture™ Exosome ELISA Kit (Streptavidin HRP) (Product code: 298-80601). Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) was used as a primary antibody. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.

03689930_img02.png


[Sample] Human serum or human heparin plasma
[Primary antibody]Anti-CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953) (1:4,000)
[Detection] HRP-conjugated streptavidin and TMB solution in the ELISA kit

Exosomes in human serum and human heparin plasma were detected concentration-dependently with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953).

Directly Detection of exosomes in cell culture supernatant

Exosomes in cell culture supernatant of COLO201 cells were detected using PS Capture™ Exosome ELISA Kit (Streptavidin HRP) (Product Code: 298-80601). As primary antibodies, Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) and three competitive anti CD9 antibodies (biotin conjugated) were used. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.

03689930_img01.png


[Sample] cell culture supernatant of COLO201
[Primary antibody] Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) (1:4,000)and competitive biotin conjugated antibodies
[Detection] HRP-conjugated streptavidin and TMB solution in the ELISA kit

Anti-CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) showed the best signal/background ratio.

Western blot

Detection of exosomes isolated from serum, heprin plasma and COLO201 cells

CD9 proteins on exosomes isolated using MagCapture™ Exosome Isolation Kit PS (Product Code: 293-77601) were detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) using a Western Blot method in non-reducing conditions.

03689930_img03.png


[Sample] Exosomes from human serum, human heparin plasma and cell culture supernatant of COLO201
[Gel] SuperSep™ Ace, 10-20%, 17well (Product code: 198-15041)
[Primary antibody] Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953) (1:1,000)
[Detection] HRP-Streptavidin and ImmunoStar™ Zeta (Product Code: 295-72404)

CD9 protein (24kDa) in each samples was detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953).

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
It is biotin-labeled antibody of rat monoclonal antibody against CD9 established by DNA immunization. CD9 is one of the membrane proteins called tetraspanin that has four transmembrane domain and is known as a marker protein of the extracellular vesicles. This product is applicable to ELISA and Western blotting.
Instructions [ELISA] 1 : 2,000 - 1 : 8,000
[Western Blotting] 1 : 1,000 - 1 : 4,000 ( Please use this antibody under non-reducing condition. )

Property

Specificity Human CD9 ( It shows little reactivity with rat CD9.)
Composition 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide, pH7.2.
Concentration On the label

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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