Anti CD9, Rat Monoclonal Antibody (30B) , Biotin Conjugated
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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20uL
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100uL
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In stock in Japan |
Document
Product Information
| Cross Reactivity | Human [Note] It shows a little reactivity with rat CD9. |
|---|---|
| Clone No. | 30B |
| Host | Rat |
| Subclass | IgG2b |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2 |
| Label | Biotin |
▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".
Application
| ELISA | 1: 2,000 - 1: 8,000 |
|---|---|
| Western Blot | 1: 1,000 - 1: 4,000 *Please use this antibody under non-reducing condition |
Please optimize the most appropriate concentration for your analysis and samples.
ELISA
Directly Detection of exosomes in biofluids
Exosomes in biofluids were detected using PS Capture™ Exosome ELISA Kit (Streptavidin HRP) (Product code: 298-80601). Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) was used as a primary antibody. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.
[Sample] Human serum or human heparin plasma
[Primary antibody]Anti-CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953) (1:4,000)
[Detection] HRP-conjugated streptavidin and TMB solution in the ELISA kit
Exosomes in human serum and human heparin plasma were detected concentration-dependently with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953).
Directly Detection of exosomes in cell culture supernatant
Exosomes in cell culture supernatant of COLO201 cells were detected using PS Capture™ Exosome ELISA Kit (Streptavidin HRP) (Product Code: 298-80601). As primary antibodies, Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) and three competitive anti CD9 antibodies (biotin conjugated) were used. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.
[Sample] cell culture supernatant of COLO201
[Primary antibody] Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) (1:4,000)and competitive biotin conjugated antibodies
[Detection] HRP-conjugated streptavidin and TMB solution in the ELISA kit
Anti-CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) showed the best signal/background ratio.
Western blot
Detection of exosomes isolated from serum, heprin plasma and COLO201 cells
CD9 proteins on exosomes isolated using MagCapture™ Exosome Isolation Kit PS (Product Code: 293-77601) were detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Product Code: 019-27953) using a Western Blot method in non-reducing conditions.
[Sample] Exosomes from human serum, human heparin plasma and cell culture supernatant of COLO201
[Gel] SuperSep™ Ace, 10-20%, 17well (Product code: 198-15041)
[Primary antibody] Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953) (1:1,000)
[Detection] HRP-Streptavidin and ImmunoStar™ Zeta (Product Code: 295-72404)
CD9 protein (24kDa) in each samples was detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated(Product Code: 019-27953).
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. It is biotin-labeled antibody of rat monoclonal antibody against CD9 established by DNA immunization. CD9 is one of the membrane proteins called tetraspanin that has four transmembrane domain and is known as a marker protein of the extracellular vesicles. This product is applicable to ELISA and Western blotting. |
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| Instructions | [ELISA] 1 : 2,000 - 1 : 8,000 [Western Blotting] 1 : 1,000 - 1 : 4,000 ( Please use this antibody under non-reducing condition. ) |
Property
| Specificity | Human CD9 ( It shows little reactivity with rat CD9.) |
|---|---|
| Composition | 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide, pH7.2. |
| Concentration | On the label |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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