Anti Iba1, Goat
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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100uL
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Antibody Information
| Application | Immunohistochemistry (frozen section) 1:200-1,000 Immunohistochemistry (paraffin section) 1:200-1,000 Western Blotting 1:1,000 |
Species reactivity | Mouse, Rat | Clonality | Polyclonal |
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| Immunogen | Synthetic peptide C-terminal of Iba1 |
Concentration | 0.5-0.7 mg/mL | Label | Unconjugated |
| Antigen synonyms | Allograft inflammatory factor 1, AIF1, IRT1 | Host | Goat | ||
| Preparation | Purified by the antigen affinity chromatography from goat antisera and prepared in TBS solution. Contains no preservatives and stabilizers. | ||||
| Background | Iba1 is a macrophage- and microglial-specific calcium binding protein which molecular weight is 17,000, widely used as a marker for microglia. | ||||
Application Data
Immunohistochemistry (fluorescent staining)
Immunohistochemistry of frozen section in mouse model of Alzheimer’s disease (APPNL-G-F mouse) brain cerebral neocortex using anti Iba1, goat polyclonal antibody at dilution of 1:1,000, and anti goat IgG, Alexa Flour 488-conjugated. Aβ were stained by 0.001% FSB solution(Amyloid fluorescent probe). The data were provided by Dr. Sakakibara, National Center for Geriatrics and Gerontology in Japan.
Immunohistochemistry of frozen section in rat(left) and mouse(right) brain cerebral cortex using anti Iba1, goat polyclonal antibody at dilution of 1:250, and anti goat IgG, Alexa Flour 488-conjugated.
The data were provided by Dr. Nakajima from Soka University in Japan.
Immunohistochemistry(DAB staining)
Sample: paraffin section of mouse brain frontal lobe
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, biotin-conjugated
Antigen retrieval: 10mM citric acid buffer(pH6.0), 90℃, 10min
Western Blotting
The data were provided by Dr. Nakajima from Soka University in Japan.
Sample:
Microglia from rat primary culture 10μg
Neuron from rat primary culture 10μg
Astrocyte from rat primary culture 10μg
Cerebral cortex from rat brain 100μg
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, HRP-conjugated
Overview / Applications
| Outline | Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage and microglia.This product is goat polyclonal antibody that specifically recognize Iba1, and is available for a microglial marker. Antigen: synthetic peptide(C-terminal of Iba1) Cross-reactivity: mouse, rat Applications: Immunohistochemistry(frozen section) 1:200-1,000 Immunohistochemistry(paraffin section) 1:200-1,000 Western Blotting 1:1,000 References 1)Karen, M. R., et al.: J. Neuroimmunol.,338, 577082(2020). Dexamethasone attenuates inflammatory-mediated suppression of B2-adrenoceptor expression in rat primary mixed glia https://pubmed.ncbi.nlm.nih.gov/31707103/ ICC, rat cell 2) Saini, Y., et al.: Cytotechnology, 4, 589(2020). IL-4 and IL-10 promotes phagocytic activity of microglia by up-regulation of TREM2 https://pubmed.ncbi.nlm.nih.gov/32623621/ ICC, mouse cell 3) Orli, T. Z., et al.: Theranostic, 11, 1, 346(2021). A new ketogenic formulation improves functional outcome and reduces tissue loss following traumatic brain injury in adult mice https://pubmed.ncbi.nlm.nih.gov/33391479/ IHC, mouse brain Cat. No. was wrong in this paper 4) Kim, J., et al.: Front. Cell. Neurosci., 15, 638098 (2021). Key Role of Microglial Matrix Metalloproteinases in Choroidal Neovascularization https://pubmed.ncbi.nlm.nih.gov/33716674/ IHC, mouse eye 5) Garima, S., et al.: J. Neuroinflammation, 18, 1, 69(2021). Fetal inflammation induces acute immune tolerance in the neonatal rat hippocampus https://pubmed.ncbi.nlm.nih.gov/33706765/ IHC, rat brain 6) Jing, Z., et al.: Aging Cell. 6, e13380(2021). https://pubmed.ncbi.nlm.nih.gov/34080759/ IHC, mouse brain |
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Property
| Appearance | Liquid |
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Manufacturer Information
Alias
- Anti Iba1, Goat Polyclonal Antibody
Anti AIF1, Goat Polyclonal Antibody
Anti IRT1, Goat Polyclonal Antibody
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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