Anti Dog Podoplanin, Monoclonal Antibody (PMab-38)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Dog
- Host :
- Mouse
- Clone No. :
- PMab-38
- Classification :
- Monoclonal
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100ug
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In stock in Japan |
Document
Overview
This is the first monoclonal antibody generated against dog podoplanin. It hardly react with dog lymphatic endothelial cells (normal cells), while gives an excellent staining (IHC) of glomerular podocytes, Bowman’s capsules, squamous cell carcinoma cells, cancer-associated fibroblasts, and lymphatic endothelium cells (melanoma cells). It is suitable for detection of podoplanin derived from cancer cells in dogs.
- Clone No.: PMab-38
- Immunized animal: Mouse
- Isotype: IgG1・κ
- Antibody concentration: Approximately 1 mg/mL (indicated on the label)
- Species cross-reactivity: Dog
- Specificity: Dog podoplanin (Tyr67-Glu68)
- Formulation: Liquid/PBS containing sodium azide.
- Application: Western Blot （1μg/mL～）
ELISA
Flow Cytometry (1μg/mL～)
Immunohistochemistry (10μg/mL～)
※Please consider the optimum operating conditions for each experiment.
References
- Honma, R. et al., "Specific detection of dog podoplanin expressed in renal glomerulus by a novel monoclonal antibody PMab-38 in immunohistocheistry.", Monoclon. Antib. Immunodiagn. Immunother., 35(4), 212-216 (2016).
- Kaneko, M.K. et al., "PMab-38 recognizes canine podoplanin of squamous cell carcinomas.", Monoclon. Antib. Immunodiagn. Immunother., 35(5), 263-266 (2016).
- Ogasawara, S. et al., "Podoplanin expression in canine melanoma.", Monoclon. Antib. Immunodiagn. Immunother., 35(6), 304-306 (2016).
- Chang, Y.W. et al., "Epitope mapping of monoclonal antibody PMab-38 against dog podoplanin.", Monoclon. Antib. Immunodiagn. Immunother., 36(6), 291-295 (2017).
- Mukai, C. et al., "Chromatin run-on sequencing analysis finds that ECM remodeling plays an important role in canine hemangiosarcoma.", BMC Vet. Res., 16, 206 (2020).
Property
| Appearance | Liquid |
|---|---|
| Specificity | Renal glomerulus and Bowman's capsule Cancer-associated fibroblasts in melanoma tissues Lymphatic endothelial cells in melanoma tissues Squamouse cell carcinomas *Not react with Lymphatic endothelial cells in normal tissues. |
| Composition | PBS, including Sodium azide |
Antibody Information
| Antigen | Podoplanin |
|---|---|
| Antigen Alias | Podoplanin HT1A-1 OTS8 PA2.26 PSEC0025 T1-ALPHA T1A T1A-2 PDPN podoplanin, transcript variant 1 GP36, T1A-2, GP40, Gp38, PA2.26, OTS8,T1A, HT1A-1 Podoplanin (PDPN) AGGRUS GP36 GP38 GP40 |
| Subclass | IgG1,k |
| Labeling | Unlabeled |
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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