Anti Amyloid β40, Monoclonal Antibody(BA27)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Human
- Cross‒Reactivity :
- Human, Mouse, Rat
- Host :
- Mouse
- Clone No. :
- BA27
- Classification :
- Monoclonal
- Application :
- WB(1:100 - 1,000), IHC(1:500 - 5,000), ELISA(1:100 - 1,000)
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10uL
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50uL
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In stock in Japan |
Document
Product Overview
Amyloid β (Aβ) is a peptide of approximately 40 amino acids and is the major component of senile plaques, a pathological hallmark of Alzheimer's disease. Aβ is classified into several isoforms based on amino acid length, each differing in aggregation tendency and cytotoxicity. The amino acid sequence of Aβ also varies across animal species, and these differences are believed to influence the aggregation tendency of endogenous Aβ. It is therefore important to select an appropriate antibody based on the Aβ isoform of interest when designing experiments.
Fujifilm Wako offers anti-Aβ mouse monoclonal antibodies originally developed by Takeda Pharmaceutical Company. Thanks to their excellent specificity and reactivity, these antibodies are widely used by researchers around the world and have been featured in numerous publications. “Anti Amyloid β40, Monoclonal Antibody(BA27)” is a mouse monoclonal antibody that recognizes the C-terminus of Aβ40 in humans, mice and rats. It can be used for ELISA, immunohistochemistry and Western blotting.
Antibody Information
| Clonality | Monoclonal (Clone No. BA27) |
|---|---|
| Antigen | Synthetic peptide (1-40 aa of human Aβ) |
| Host | Mouse |
| Formulation | PBS, 0.05% Sodium azide |
| Conjugate | Unconjugated |
| Cross-reactivity | Human, Mouse, Rat |
| Application | ELISA 1:100-1,000 Immunohistochemistry 1:500-5,000 Western blotting 1:100-1,000 |
Data
Immunohistochemistry
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BAN50
(Dilution 1:1,000) -
BNT77
(Dilution 1:1,000) -
BA27
(Dilution 1:1,000) -
BC05
(Dilution 1:100,000)
Sample
- Species:
- 15-month-old APP/PS1 line AD model mouse, B6.Cg-Tg (APPswe, PSEN1dE9) 85Dbo/Mmjax
- Site:
- Piriform cortex
- Sample:
- Paraffin sections
Condition
- Antigen retrieval- BAN50:
- Microwave treatment in citrate buffer + proteinase K
- Detection
Biotin-conjugated antibody, Avidin-biotin system + DAB staining
Data by courtesy of Drs. Kokawa, Hashimoto and Iwatsubo, Graduate School of Medicine, University of Tokyo
Western blotting
(Dilution 1:1,000)
(Dilution 1:1,000)
(Dilution 1:1,000)
Sample
- Lane 1-3:
- TBS brain extracts from WT mice
- Lane 4-6:
- TBS brain extracts from 15-monthsold APP/PS1 AD model mice, B&.Cg-Tg (APPswe. PSEN1dE9)
Condition
- Applied amount:
- 30 μg/Lane
Data by courtesy of Drs. Hashimoto and Iwatsubo, Graduate School of Medicine, University of Tokyo
FAQ
About Antibody
- What is the subclass of this antibody?
- The subclass of this antibody is IgG2a・κ.
- Has this antibody been affinity purified?
- Yes, it has been affinity purified using Protein A.
- What is the concentration of this antibody?
- The specification for protein concentration is 0.9-1.3 mg/mL.
Troubleshooting
- No staining is observed in immunohistochemistry of wild-type mice.
- Aβ hardly accumulates in wild-type mice. Please use mice engineered to overexpress human Aβ, etc.
- No band is detected in Western blotting of wild-type mice.
- Aβ hardly accumulates in wild-type mice. Please use mice engineered to overexpress human Aβ, etc.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. Amyloid β(Aβ) is a peptide which consists of about 40 amino acids. Aβ is considered the cause of Alzheimer's disease, because it is a major component of senile plaques formed in brains of Alzheimer's disease patients. Aβ has several types differing in lengthe of the amino acid. The main types are Aβ 40 (40amino acid), Aβ 42 (42 amino acid), and Aβ 43 (43 amino acid). This item is a mouse monoclonal antibody that specifically reacts with Aβ 40. The antibody was developed by Takeda Pharmaceutical Company Limited. [Reactivity] Reacts with Amyloid β 40. [Antigen] 1-40 a.a of human Amyloid β [Species cross reactivity] Human, Mouse and Rat [Clone No.] BA27 [Host] Mouse [Isotype] IgG2a·κ [Purification] Protein A-affinity purified [Concentration] Indicated on the lable [Formulation] PBS with 0.05% Sodium Azide |
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Property
Antibody Information
| Antigen | Amyloid β (aa1-40) |
|---|---|
| Antigen Alias | β Amyloid 1-40 Amyloid β 1-40 Amyloid Beta (1-40) b-Amyloid (1-40) Abeta 40 (beta amyloid 1-40) Amyloid β (aa1-40) |
| Subclass | IgG2a,k |
| Labeling | Unlabeled |
Manufacturer Information
Alias
- Anti-β Amyloid(40), Mouse Monoclonal Antibody（Clone No. BA27）
Anti-Aβ(40), Mouse Monoclonal Antibody（Clone No. BA27）
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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