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Anti CD81, Monoclonal Antibody (17B1)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
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Product Number
Package Size
Price
Inventory
Distributor
015-27771
Barcode No
4548995071064
20uL
Discontinued
Distributor
011-27773
Barcode No
4548995071071
100uL
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In stock in Japan

Document

SDS ...all
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Information

Cross Reactivity Human, Bovine
[Note] It dose not react with mouse and rat CD81
Clone No. 17B1
Host Mouse
Subclass IgG2b
Formulation 1×TBS aqueous solution with 50% glycerol, 0.05%w/v sodium azide, pH7.4
Label unlabeled

▼Evaluating a reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive".

Application

ELISA 1 : 2,000 – 1 : 16,000
Western Blot 1 : 1,000 – 1 : 8,000 *Please use this antibody under non-reducing condition
Flow Cytometry 1 : 250 – 1 : 1,000
Immunoprecipitation 5 ‒ 50μg/assay

Please optimize the most appropriate concentration for your analysis and samples.

Application Data

Western Blot

Exosomes (1.25 to 20 ng) isolated and purified from COLO201 cell conditioned medium using MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601) were detected with this antibody or competitor A.

Isolation of Exosomes: MagCapture™ Exosome Isolation Kit PS (Code No. 293-77601)
Gel: SuperSep™ ACE 10-20%, 17well (Code No. 198-15041)
Runnning Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk / PBS-T
Chemiluminescent reagent: ImmunoStar® Zeta (Code No. 295-72404)
Primary Antibody: 2,000-fold dilution
Secondary Antibody: Anti Mouse IgG (H+L), Peroxidase Conjugated, 10,000-fold dilution.

ELISA

Exosomes in pretreated 500-fold diluted COLO201 cell conditioned medium were measured using this antibody or competitor A as the primary antibody. As reagents other than the primary antibody, ones included in PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) (Code No. 297-79201) were used.

※ Pretreatment condition: 10,000 x g, 30min.

Flow Cytometry

From pretreated 8-fold diluted COLO201 cell conditioned medium, exosomes were isolated using PS Capture™ Exosome Flow Cytometry Kit (Code No. 297-79701) and subjected to detection of the exosome surface antigen by flow cytometry using this antibody or competitor A and fluorescence-labeled anti-mouse IgG antibody (Code No. 569-79631).

※ Pretreatment condition: 10,000 x g, 30min.

Immunoprecipitation

Exosomes in pretreated COLO201 cell conditioned medium were captured by immunoprecipitation using this antibody or competitor A and detected using HRP-labeled anti-CD9 antibody (competitor B).

※ Pretreatment condition: 10,000 x g, 30min.

Gel: SuperSep™ ACE 10-20%, 17well (Code No. 198-15041)
Running Buffer: SDS-PAGE Buffer, pH8.5 (Code No. 192-16801)
Blocking: 3% Skim milk/PBS-T
Chemiluminescent reagent: ImmunoStar® Zeta (Code No. 295-72404)
HRP conjugated Anti-CD81 antibody: 1,000-fold dilution
COLO201 cell culture supernatant: 1 mL
Antibody amount: 5 μg

This product successfully detected CD81 on the exosome surface at a high sensitivity. Also, CD81 positive exosomes were efficiently collected.

Overview / Applications

Property

Specificity Human CD81, Bovine CD81
*Does not react with mouse and rat CD81.
[Reactivity confirmation condition]
The reactivity of this antibody against the serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive.

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

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