Anti CD133, Monoclonal Antibody (CMab-43)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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100ug
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In stock in Japan |
Document
Product Overview
CD133 also known as Prominin-1 or AC133 is a protein belonging to the Prominin family, and was identified as a cell surface marker of hematopoietic stem cells. It is known as a marker for cancer stem cells (CSCs) derived from solid tumors. Therefore, it has been used as a marker for isolating stem cells from a wide range of human and mouse tissues and tumors.
This product is a mouse monoclonal antibody against human CD133. It can be used for immunohistochemistry, flow cytometry, and Western blotting.
Antibody Information
| Clonality | Monoclonal |
|---|---|
| Antigen | Human CD133, recombinant (expressed in LN229 cells) |
| Host | Mouse |
| Formulation | PBS, 0.1% sodium azide |
| Concentration | 1.0 mg/mL |
| Subclass | IgG2a · κ |
| Conjugate | Unconjugated |
| Cross-reactivity | Human |
| Application |
Flow Cytometry:1 μg/mL
IHC:1 μg/mL
WB:1 μg/mL
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Application Data
- Sample: Colon cancer tissue
- Antibody concentration: 1 μg/mL
CMab-43 can be used to detect CD133-expressing cells when the human glioblastoma cell line LN229 or the Chinese hamster ovary cell subclone CHO-K1 is transfected with the CD133 gene.
- Antibody concentration:
- 1 μg/mL
- Secondary Antibody:
- Alexa Fluor® 488 fluorescently labeled anti-mouse IgG antibody (Alexa Fluor® is a registered trademark of Invitrogen Inc.)
Note: The data for immunohistochemistry, flow cytometry and Western blotting are reproduced from reference 1) under CCBY 4.0.
- Antibody concentration:
- 1 μg/mL
- Secondary Antibody:
- Peroxidase-conjugated anti-mouse IgG antibody
Reference
- Itai, S. et al.: Monoclon. Antib. Immunodiagn. Immunother., 36 (5), 231 (2017).
Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry
Property
| Concentration | Protein : 1.0mg/mL |
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Alias
- AC133
PROML1
Prominin 1
PROM1
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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