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Anti CD133, Monoclonal Antibody (CMab-43)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
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Distributor
012-28881
Barcode No
4548995105974
100ug
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In stock in Japan

Document

Package Insert
Certificate of Analysis

Product Overview

CD133 also known as Prominin-1 or AC133 is a protein belonging to the Prominin family, and was identified as a cell surface marker of hematopoietic stem cells. It is known as a marker for cancer stem cells (CSCs) derived from solid tumors. Therefore, it has been used as a marker for isolating stem cells from a wide range of human and mouse tissues and tumors.
This product is a mouse monoclonal antibody against human CD133. It can be used for immunohistochemistry, flow cytometry, and Western blotting.

Antibody Information

Clonality Monoclonal
Antigen Human CD133, recombinant (expressed in LN229 cells)
Host Mouse
Formulation PBS, 0.1% sodium azide
Concentration 1.0 mg/mL
Subclass IgG2a · κ
Conjugate Unconjugated
Cross-reactivity Human
Application
Flow Cytometry:1 μg/mL
IHC:1 μg/mL
WB:1 μg/mL

Application Data

Immunohistochemistry
Immunohistochemistry
  • Sample: Colon cancer tissue
  • Antibody concentration: 1 μg/mL
Flow Cytometry
Flow Cytometry

CMab-43 can be used to detect CD133-expressing cells when the human glioblastoma cell line LN229 or the Chinese hamster ovary cell subclone CHO-K1 is transfected with the CD133 gene.

Antibody concentration:
1 μg/mL
Secondary Antibody:
Alexa Fluor® 488 fluorescently labeled anti-mouse IgG antibody (Alexa Fluor® is a registered trademark of Invitrogen Inc.)

Note: The data for immunohistochemistry, flow cytometry and Western blotting are reproduced from reference 1) under CCBY 4.0.

Western Blotting
Western Blotting
Antibody concentration:
1 μg/mL
Secondary Antibody:
Peroxidase-conjugated anti-mouse IgG antibody

Reference

  1. Itai, S. et al.: Monoclon. Antib. Immunodiagn. Immunother., 36 (5), 231 (2017).
    Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry

Property

Concentration Protein : 1.0mg/mL

Alias

  • AC133
    PROML1
    Prominin 1
    PROM1

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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