Anti 6×Histidine, Monoclonal Antibody(9F2)

for Immunochemistry

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

Host :: Mouse
Clone No. :: 9F2

Classification :: Monoclonal

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Structural Formula
Label
Packing

Product Number	Package Size	Price	Inventory
Distributor 014-21864 Barcode No 4548995013446	1mg		Discontinued	Certificate of Analysis
Distributor 010-21866 Barcode No 4548995013453	5mg		Discontinued	Certificate of Analysis
Distributor 010-21861 Barcode No 4987481478015	200ug	Inquire	In stock in Japan	Certificate of Analysis

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Antibody Information
Manufacturer Information
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Product Specification Sheet

Spectral Data

Certificate of Analysis

Calibration Certificate

Application

Overview / Applications

Outline	This product is used for detection of C terminal 6His fusion recombinant protein by using immunoprecipitation and western blot. This product is for research use only. Do not administer it to human. The 6xhistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein. Monoclonal Anti 6xhistidine (6xHis) antibody was produced by hybridoma clone 9F2. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. [Concentration] Indicated on the label. [Formulation] 1xPBS aqueous solution with 50% glycerol. [Clone No.] 9F2 [Subclass] IgG₁ [Specificity] Specific for C-terminal of 6xHis tagged recombinant protein. [Working Concentration] Western blot 0.2 - 2 µg/mL Immunoprecipitation 2 - 3 µg/20 µL Protein G beads slurry
Instructions	Western Blot(0.5ug/mL), Immunoprecipitation(2.5ug/300mL cell lysis solution).

Outline

This product is used for detection of C terminal 6His fusion recombinant protein by using immunoprecipitation and western blot.
This product is for research use only. Do not administer it to human.

The 6xhistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein.
Monoclonal Anti 6xhistidine (6xHis) antibody was produced by hybridoma clone 9F2. This clone was established by fusing mouse myeloma cells and lymphocytes of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.

[Concentration]
Indicated on the label.

[Formulation]
1xPBS aqueous solution with 50% glycerol.

[Clone No.]
9F2

[Subclass]
IgG₁

[Specificity]
Specific for C-terminal of 6xHis tagged recombinant protein.

[Working Concentration]
Western blot 0.2 - 2 µg/mL
Immunoprecipitation 2 - 3 µg/20 µL Protein G beads slurry

Instructions

Western Blot(0.5ug/mL), Immunoprecipitation(2.5ug/300mL cell lysis solution).

Property

Appearance	Liquid
Composition	PBS aqueous solution with 50% glycerol.
Concentration	0.4 - 0.6mg/mL Protein

Antibody Information

Antigen	6×Histidine
Antigen Alias	6HIS Peptide 6Xhis 6xHis (Hexahistidine Polyhistidine) 6-His 6-His (HHHHHH) 6-His tag 6X-HIS Epitope Tag (His)6 [His-Tag] 6x-His Tag 6×Histidine

Manufacturer Information

Alias

Anti 6×His, Monoclonal Antibody

Related Information

Product List

Product Name
Anti 6×Histidine, Monoclonal Antibody(9C11)	for Immunochemistry
Anti 6×Histidine, Monoclonal Antibody(21-48)	for Immunochemistry
Anti 6×Histidine, Monoclonal Antibody(28-75)	for Immunochemistry
Anti 6×Histidine, Monoclonal Antibody(9F2), Peroxidase Conjugated	for Immunochemistry
Anti 6×Histidine, Monoclonal Antibody(9C11), Peroxidase Conjugated	for Immunochemistry
Ni-NTA Agarose	for Genetic Research
Ni-NTA Cartridge	for Genetic Research
Ni-NTA Agarose HP	for Genetic Research
6×Histidine Peptide	for Genetic Research
Imidazole	for Molecular Biology

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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