Anti CD9, Rat Monoclonal Antibody(77B)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
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20uL
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100uL
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In stock in Japan |
Document
Product Information
| Cross Reactivity | Human Note: This antibody doesn’t recognize the mouse and bovine CD9. It shows a little reactivity with rat CD9. |
|---|---|
| Clone No. | 77B |
| Host | Rat |
| Subclass | IgG2b |
| Formulation | 1×TBS aqueous solution with 50% glycerol, 0.05w/v% sodium azide, pH 7.2. |
| Label | Unconjugated |
▼Evaluating the reactivity
The reactivity of this antibody against a serum of each organisms was measured by ELISA.
When the signal value was the same level as the blank, it was judged as "non-reactive”.
Application
| ELISA | (Detection) 1: 2,000-1: 16,000 (Immobilization) 1: 100- 1: 1,000 |
|---|---|
| Western Blot | 1: 1,000 – 1: 8,000 *Please use this antibody under non-reducing condition. |
| Flow Cytometry | 1: 200 – 1: 2,000 |
Please optimize the most appropriate concentration for your analysis and samples.
Application Data
▼ELISA
Immobilized this product and competitors anti CD9 antibodies, and then compared the capture performance using ELISA.
Immobilized antibodies: Anti CD9, Rat Monoclonal Antibody(77B), Competitor’s anti CD9 antibody (each 5 μg/mL)
Exosome samples: Exosome, from COLO201 cells, purified [Code No. 052-09301]
Detection antibody: Anti CD9, Rat Monoclonal Antibody(77B), Biotin-conjugated
| This product shows higher signal value and S/B ratio than competitors. |
▼Western Blot
CD9 proteins from exosomes were detected by western blot.
Samples: Exosomes from cell culture supernatant of COLO201, human serum and human heparin plasma
Primary antibody: Anti CD9, Rat Monoclonal Antibody(77B) (1 μg/mL)
Secondary antibody: Anti Rat IgG(H+L), Donkey, Peroxidase-conjugated [Code No. 564-73961] (×20,000 diution)
Detection reagent: Immunostar® Zeta [Code No. 291-72401]
| CD9 protein (24kDa) in each sample (non-reducing condition) was detected with this product. |
Overview / Applications
| Outline | This product is monoclonal antibody to CD9 that was produced from hybridoma (clone No. 77B). CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily (TM4SF) and, is found on the surface of exosomes. This is known to form a complex with integrins and other TM4SF, can modulate cell adhesion and migration. Also, this induces platelet activation and aggregation. This product is intended for laboratory use only. This product is monoclonal antibody to CD9 that was produced from hybridoma (clone No. 77B). CD9 is a cell surface glycoprotein which belongs to the tetraspanin superfamily (TM4SF) and, is found on the surface of exosomes. This is known to form a complex with integrins and other TM4SF, can modulate cell adhesion and migration. Also, this induces platelet activation and aggregation. This antibody does't show cross reactivity with bovine. And, This can be used as immobilized antibody in ELISA. This product is intended for laboratory use only. |
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| Application | Western blotting 1: 1,000-1: 8,000 *Use this antibody under non-reducing condition. ELISA (detection) 1: 2,000-1: 16,000 (immobilization) 1: 100-1: 1,000 Flow cytometry 1: 200-1: 2,000 Please optimize the most appropriate concentration for your analysis and samples. |
Property
| Specificity | Human CD9 Note: It shows a little reactivity with rat CD9. |
|---|---|
| Composition | 1xTBS aqueous solution with 50w/v% glycerol, 0.05w/v% sodium azide. |
| Concentration | Indicated on the label. |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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