Anti Iba1, Rabbit (for Western Blotting)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Rat
- Cross‒Reactivity :
- human, mouse, rat
- Host :
- Rabbit
- Application :
- WB
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50ug
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Document
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to Iba1. Preparation: purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. It contains no preservatives and stabilizers. Specificity:specific to microglia. Reactive with human, mouse and rat Iba1. Working Concentration: Westernblot 0.5-1 ug/mL Storage: Keep at -20C. After opening, aliquot contents and freeze at -20C. Package size: 50ug References: Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996). Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998). Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000). Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001). Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026 (2002). |
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| Preparation | Rabbit Anti Iba1 antibody is raised against synthetic peptide corresponding to C-terminus of Iba1. Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers. |
Property
| Appearance | Prepared in TBS solution. Contains no preservatives and stabilizers. Colorless liquid |
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| Specificity | Reactive with human, mouse and rat Iba1. |
| Concentration | 0.5-0.6mg/mL |
Antibody Information
| Antigen | Iba1 |
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| Antigen Alias | Iba1 Iba 1 |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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