Anti Human Amyloid β, Monoclonal Antibody(BAN50)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Human
- Host :
- Mouse
- Clone No. :
- BAN50
- Classification :
- Monoclonal
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10uL
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50uL
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In stock in Japan |
Document
Product Overview
Amyloid β (Aβ) is a peptide of approximately 40 amino acids and is the major component of senile plaques, a pathological hallmark of Alzheimer's disease. Aβ is classified into several isoforms based on amino acid length, each differing in aggregation tendency and cytotoxicity. The amino acid sequence of Aβ also varies across animal species, and these differences are believed to influence the aggregation tendency of endogenous Aβ. It is therefore important to select an appropriate antibody based on the Aβ isoform of interest when designing experiments.
Fujifilm Wako offers anti-Aβ mouse monoclonal antibodies originally developed by Takeda Pharmaceutical Company. Thanks to their excellent specificity and reactivity, these antibodies are widely used by researchers around the world and have been featured in numerous publications. “Anti Human Amyloid β, Monoclonal Antibody(BAN50)” is a mouse monoclonal antibody that recognizes the N-terminus of Aβ in humans. It can be used for ELISA, immunocytochemistry, immunohistochemistry, immunoprecipitation and Western blotting.
Antibody Information
| Clonality | Monoclonal (Clone No. BAN50) |
|---|---|
| Antigen | Synthetic peptide (1-16 aa of human Aβ) |
| Host | Mouse |
| Formulation | PBS, 0.05% Sodium azide |
| Conjugate | Unconjugated |
| Cross-reactivity | Human |
| Application | ELISA 1:100-500 Immunocytochemistry 1:500-2,000 Immunohistochemistry 1:500-2,000 Immunoprecipitation 1:1,000 Western blotting 1:500-2,000 |
Data
ELISA
Measurement in Human and Mouse Plasma
Blood was collected using EDTA-2K vacuum blood collection tubes, and plasma was separated by centrifugation at 5,000 × g for 15 minutes at 4℃. The plasma was then stored at -80℃ until use. Prior to measurement, the samples were diluted 4-fold with the standard dilution buffer provided in the kit, and Aβ levels were measured.
Combinations of our anti-Aβ antibodies used in the ELISA
| Clone No. | Cross-reactivity (Human) | Cross-reactivity (Mouse/Rat) | ELISA Kits (Commercially Available) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Capture | Detection | Aβ (1-40) | Aβ (1-42) | Aβ (x-40) | Aβ (x-42) | Aβ (1-40) | Aβ (1-42) | Aβ (x-40) | Aβ (x-42) | Product No. | Product Name | |
| Aβ(x-40) | BNT77 | BA27 (F(ab’)2) | ● | - | ● | - | ● | - | ● | - | 294-64701 | Human/Rat β Amyloid(40) ELISA Kit Wako Ⅱ |
| Aβ(x-42) | BNT77 | BC05 (Fab’) | - | ● | - | ● | - | ● | - | ● | 292-64501 | Human/Rat β Amyloid(42) ELISA Kit Wako, High Sensitive |
| Aβ(1-40) | BAN50 | BA27 (F(ab’)2) | ● | - | - | - | - | - | - | - | 298-64601 | Human β Amyloid(1-40) ELISA Kit Wako Ⅱ |
| Aβ(1-42) | BAN50 | BC05 (Fab’) | - | ● | - | - | - | - | - | - | 296-64401 | Human β Amyloid(1-42) ELISA Kit Wako, High Sensitive |
Detection of High Molecular Aβ Oligomers
Fujifilm Wako's High Molecular Weight Amyloid β Oligomer ELISA Kit Wako Ver.2 (Product No. 290-82001) is designed to measure Aβ oligomers in human serum, plasma, and CSF. Utilizing an antibody with high affinity for Aβ (clone No. BAN50), this kit specifically measures Aβ oligomers composed of nine or more monomers1-2).
The Aβ1-42 peptide (monomer) was incubated overnight under refrigeration and then separated by ultrafiltration into two fractions: above 30 kDa (nonamers or larger, Fraction A) and below 30 kDa (octamers or smaller, Fraction B). Each fraction was then analyzed by High Molecular Weight Amyloid β Oligomer ELISA Kit Wako Ver.2.
[Result]
In the ELISA, Fraction B (octamers or smaller) showed almost no signal, while Fraction A (nonamers or larger) exhibited a strong signal.
Measurement in Clinical Samples
High molecular weight Aβ oligomers were measured in the CSF of non-dementia patients (Control), patients with mild cognitive impairment (MCI), and AD patients (AD) using High Molecular Weight Amyloid β Oligomer ELISA Kit Wako Ver.2 (Product No. 290-82001). The correlation between Aβ oligomer levels and mini-mental state examination (MMSE) scores was analyzed.
MMSE Score
≦23 : Dementia suspected
24-27 : MCI suspected
28-30 : Normal
[Result]
Significant differences were observed in the levels of high molecular weight Aβ oligomers among the Control, MCI, and AD groups. Furthermore, the levels of Aβ oligomers showed a negative correlation with MMSE scores.
Immunohistochemistry
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BAN50
(Dilution 1:1,000) -
BNT77
(Dilution 1:1,000) -
BA27
(Dilution 1:1,000) -
BC05
(Dilution 1:100,000)
Sample
- Species:
- 15-month-old APP/PS1 line AD model mouse, B6.Cg-Tg (APPswe, PSEN1dE9) 85Dbo/Mmjax
- Site:
- Piriform cortex
- Sample:
- Paraffin sections
Condition
- Antigen retrieval- BAN50:
- Microwave treatment in citrate buffer + proteinase K
- Detection
Biotin-conjugated antibody, Avidin-biotin system + DAB staining
Data by courtesy of Drs. Kokawa, Hashimoto and Iwatsubo, Graduate School of Medicine, University of Tokyo
Western blotting
Detection of Soluble Aβ in Western blotting
(Dilution 1:1,000)
(Dilution 1:1,000)
(Dilution 1:1,000)
Sample
- Lane 1-3:
- TBS brain extracts from WT mice
- Lane 4-6:
- TBS brain extracts from 15-monthsold APP/PS1 AD model mice, B&.Cg-Tg (APPswe. PSEN1dE9)
Condition
- Applied amount:
- 30 μg/Lane
Data by courtesy of Drs. Hashimoto and Iwatsubo, Graduate School of Medicine, University of Tokyo
Detection of High Molecular Aβ Oligomers
A: above 30 kDa
(nonamers or larger)
B: below 30 kDa
(octamers or smaller)
The Aβ1-42 peptide (monomer) was incubated overnight under refrigeration and then separated by ultrafiltration into two fractions: above 30 kDa (nonamers or larger, Fraction A) and below 30 kDa (octamers or smaller, Fraction B). Each fraction was then analyzed by Western blotting using the anti-Aβ antibody (BAN50).
[Result]
Aβ oligomers with various molecular weights were detected.
References
- Fukumoto, H. et al.: FASEB J., 24 (8) , 2716 (2010).
High-molecular-weight beta-amyloid oligomers are elevated in cerebrospinal fluid of Alzheimer patients - Kasai, T. et al.: Biochem. Biophys. Res. Commun., 422 (3) , 375 (2012).
Utilization of a multiple antigenic peptide as a calibration standard in the BAN50 single antibody sandwich ELISA for Aβ oligomers
FAQ
About Antibody
- What is the subclass of this antibody?
- The subclass of this antibody is IgG1・κ.
- Has this antibody been affinity purified?
- Yes, it has been affinity purified using Protein A.
- What is the concentration of this antibody?
- The specification for protein concentration is 0.9-1.3 mg/mL.
Troubleshooting
- No staining is observed in immunohistochemistry of wild-type mice.
- This product does not react with mouse Aβ. Please use mice engineered to express human Aβ.
- No band is detected in Western blotting of wild-type mice.
- This product does not react with mouse Aβ. Please use mice engineered to express human Aβ.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. Amyloid β(Aβ) is a peptide which consists of about 40 amino acids. Aβ is considered the cause of Alzheimer's disease, because it is a major component of senile plaques formed in brains of Alzheimer's disease patients. This item is a mouse monoclonal antibody that reacts with human Aβ. The antibody was developed by Takeda Pharmaceutical Company Limited. [Reactivity] Reacts with human Amyloid β [Antigen] 1-16 a.a of human Amyloid β [Species cross reactivity] Human No-cross reactivity to mouse and rat [Clone No.] BAN50 [Host] Mouse [Isotype] IgG1·κ [Purification] Protein A-affinity purified [Concentration] Indicated on the label [Formulation] PBS with 0.05% Sodium Azide |
|---|
Property
Antibody Information
| Antigen | Amyloid β (aa1-16) |
|---|---|
| Antigen Alias | Amyloid β (1-16) Amyloid beta-Protein (1-16) Amyloid β (aa 1-16) |
| Subclass | IgG1,k |
| Labeling | Unlabeled |
Manufacturer Information
Alias
- Anti-beta Amyloid, Mouse Monoclonal Antibody （Clone No. BAN50）
Anti-Aβ, Mouse Monoclonal Antibody （Clone No. BAN50）
Anti-Human Aβ, Mouse Monoclonal Antibody （Clone No. BAN50）
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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