Anti 6×Histidine, Monoclonal Antibody(28-75)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- Host :
- Mouse
- Clone No. :
- 28-75
- Classification :
- Monoclonal
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- Structural Formula
- Label
- Packing
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1mg
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Discontinued
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200ug
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In stock in Japan |
Document
Application
Overview / Applications
| Outline | The 6xHistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti 6xHistidine (6xHis) antibody was produced by hybridoma clone 28-75 fusing mouse myeloma cells P3U1 and lymphocytes of BALB/c mice immunized with synthetic HHHHHH peptides which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites by using Protein A affinity chromatography. Concentration: Indicated on the label. Formulation: 50 mmol/L MOPS, 150 mmol/L sodium chloride, 0.1 w/v% sodium azide. Clone No.: 28-75 Subclass: IgG3, κ Specificity: Specific for N and C terminal of 6 X His tagged recombinant protein. Working Dilution: Applicable to immunoprecipitation and a best suited antibody for immunoprecipitation. |
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| Preparation | This antibody was purified from mouse ascites by using Protein A affinity chromatography. |
| Instructions | Western Blot(1:500-1:2,000), Immunoprecipitation(1-10ug/ml ProteinG Beads), ELISA(1-2ug/mL) |
Property
| Appearance | Liquid |
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| Origin / Source | Mouse ascites |
| Specificity | Specific for N and C terminal of 6×His tagged recombinant protein. |
| Composition | 50mmol/l MOPS, 150mmol/l Sodium chloride, 0.1w/v% Sodium azide. |
| Concentration | 1.0mg/ml |
Antibody Information
| Antigen | 6×Histidine |
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| Antigen Alias | 6HIS Peptide 6Xhis 6xHis (Hexahistidine Polyhistidine) 6-His 6-His (HHHHHH) 6-His tag 6X-HIS Epitope Tag (His)6 [His-Tag] 6x-His Tag 6×Histidine |
| Subclass | IgG3･κ |
Manufacturer Information
Alias
- 6×His
His
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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