Anti 6×Histidine, Monoclonal Antibody(9C11), Peroxidase Conjugated
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Host :
- Mouse
- Clone No. :
- 9C11
- Classification :
- Monoclonal
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- Packing
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100uL
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In stock in Japan |
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500uL
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In stock in Japan |
Document
Application
Overview / Applications
| Outline | The 6xHistidine polypeptide has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti 6xHistidine (6xHis) antibody was produced by hybridoma clone 9C11. This clone was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic 6xHis peptide that was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. This antibody was conjugated with horseradish peroxidase. This product is for research use only. Do not administer it to human. A monoclonal antibody to N-terminal and C-terminal 6x polyhistidine peptide and is labeled with horse radish peroxidase (HRP). Applicable to detection of recombinant fusion proteins with N-terminal and C-terminal 6x His tag expressed in Escherichia coli, yeast and mammalian cells. Purpose of use (concentration of use): Western blot (1 in 4,000 to 1 in 16,000). |
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| Features | Detection for N and C terminal 6xHis tag. |
| Preparation | This antibody was purified from mouse ascites by using Protein A affinity chromatography. |
| Instructions | Western Blot(1:4,000-1:20,000) |
Property
| Appearance | Liquid |
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| Specificity | Specific for N and C terminal of 6xHis tagged recombinant protein. |
| Concentration | Protein : 1mg/mL |
Antibody Information
| Antigen | 6×Histidine |
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| Antigen Alias | 6HIS Peptide 6Xhis 6xHis (Hexahistidine Polyhistidine) 6-His 6-His (HHHHHH) 6-His tag 6X-HIS Epitope Tag (His)6 [His-Tag] 6x-His Tag 6×Histidine |
| Subclass | IgG1 |
| Labeling | Peroxidase |
Manufacturer Information
Alias
- 6×His
His
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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