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Y-27632, MF

for Cell Culture
Specification Assay :
98.0+% (HPLC)
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Molecular Formula :
Molecular Weight :
  • Structural Formula
  • Label
  • Packing
Product Number
Package Size
Barcode No
List Price
550.00 USD

In stock

Barcode No

In stock


Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Y-27632 Comparison Chart

ICH-Q7 ISO9001 Sterility
Y-27632 (GMP-compliant)** - Powder -
Y-27632, MF*** - - Powder
CultureSure® Y-27632 - - - Powder
CultureSure® 10mmol/L
Y-27632 Solution,
- - Liquid
Sterilized with filter

* Click to move to the PubMed Central® search results.
** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
*** The manufacturing process and analytical procedure are validated.


Solubility Data for Y-27632 dihydrochloride

C14H21N3O・2HCl・H2O = 338.27

Solvent Conc. mg/mL Conc. mmol/L
Solubility Water 10mg/mL 29.56 mmol/L
Ethanol 10mg/mL 29.56 mmol/L

Preparing Stock Solutions

C14H21N3O・2HCl・H2O = 338.27

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mmol/L 2.956 mL 14.781 mL 29.562 mL
5 mmol/L 0.591 mL 2.956 mL 5.912 mL
10 mmol/L 0.296 mL 1.478 mL 2.956 mL
50 mmol/L 0.059 mL 0.296 mL 0.591 mL


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    A ROCK inhibitor permits survival of dissociated human embryonic stem cells
  2. Masashi, H., et al. : Genes Dev, 1, 36(1-2), 84–102 (2022).
    Highly rigid H3.1/H3.2–H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells
  3. Yoshifumi, M., et al. : J Neurosci Res, 98(8), 1575–1587 (2020).).
    Zonisamide promotes survival of human‐induced pluripotent stem cell‐derived dopaminergic neurons in the striatum of female rats
  4. Kazutoshi, T., et al. : Cell Rep Methods, 2(2), 100155 (2022).
    A stress-reduced passaging technique improves the viability of human pluripotent cells
  5. Yu, K., et al. : eNeuro, 9(2), ENEURO.0442-21 (2022).
    Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells
  6. Tomoya, U., et al. : Cell Rep Med, 2(6) 100298 (2021).
    A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs
  7. Genki, N., et al. : eLife, 11, e77007 (2022).
    Robotic search for optimal cell culture in regenerative medicine
  8. Maobi, Z., et al. : Cell, 8(8), 933 (2019).
    Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube
  9. Yu, K., et al. : Front Endocrinol (Lausanne), 13, 941166 (2022).
    EpCAM Is a Surface Marker for Enriching Anterior Pituitary Cells From Human Hypothalamic-Pituitary Organoids
  10. Sho, T., et al. : STAR Protoc, 3(2), 101360 (2022).
    Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
  11. Takuro, T., et al. : Commun Biol, 5, 453, (2022).
    Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria
  12. Tomoki, Y., et al. : Mol Ther Methods Clin Dev, 10(22), 263–278 (2021).
    Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research
  13. Bin, X., et al. : J Neurochem, 153(6), e14949 (2020).
    Transplantation of iPS‐derived vascular endothelial cells improves white matter ischemic damage
  14. Hiroyuki, N., et al. : Front Physiol, 10, 818 (2019).
    Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias
  15. Yayoi, H., et al. : Front Pharmacol, 12, 680618 (2021).
    High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias
  16. Masahiko, A., et al. : Nat Commun, 12, 4507 (2021).
    Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia
  17. Maobi, Z., et al. : Biomolecules, 10(9), 1270 (2020).
    Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro—Stem Cell of Fallopian Epithelium
  18. Junko, Y., et al. : iScience, 25(7), 104538 (2022).
    StemPanTox: A fast and wide-target drug assessment system for tailor-made safety evaluations using personalized iPS cells
  19. Keitaro, K., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
    Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells
  20. Koichiro, O., et al. :Sci Rep, 8, 3615 (2018).
    Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
  21. Kengo, K., et al. : Sci Rep, 11, 22476 (2021)
    Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage
  22. Hayato, F., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
    Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient (PARK4) with SNCA triplication
  23. Yoji, K., et al. : Life Sci Alliance, 4(5), e202000974 (2021).
    GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
  24. Eri, N., et al. : Biochem Biophys Rep, 29, 101195 (2022).
    Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP
  25. Kun, H., et al. : Sci Rep, 10, 2558 (2020).
    Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells
  26. Yusuke, T., et al. : Int J Mol Sci, 20(6), 1331 (2019).
    ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells

Overview / Applications

Outline This product is for research use only. Do not administer it to human.

This product is registered in the Drug Master File in Japan.
It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).
Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improve viability of the cells after cryopreservation.
[ROCK : Rho-associated coiled-coil forming kinase]

Precautions for Use Packed on inactive gas


Appearance White - pale yellow, crystalline powder - powder
Boiling Point about 85 degrees C
Specific Rotation +2.0 - +10.0 degrees (D/20 degrees C)(c=1.0,CH3OH)
Purity Bacterial endotoxins : <0.25EU/mg

Manufacturer Information


  • (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)cyclohexanecarboxamide Dihydrochloride Monohydrate

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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