Y-27632, MF
- for Cell Culture
- Specification Assay :
- 98.0+% (HPLC)
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- CAS RN® :
- 331752-47-7
- Molecular Formula :
- C14H21N3O・2HCl・H2O
- Molecular Weight :
- 338.27
- GHS :
-
- Structural Formula
- Label
- Packing
- SDS
Comparison
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Product Number
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Package Size
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Price
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Availability
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Certificate of Analysis
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Purchase |
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5mg
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In stock in Japan |
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25mg
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Y-27632 Comparison Chart
ICH-Q7 | ISO9001 | Sterility test |
Viable count test |
Animal- derived- free |
Bacterial Endotoxin test |
Mycoplasma test |
Powder/ Liquid |
References* | |
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Y-27632 (GMP-compliant)** | ✓ | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | - |
Y-27632, MF*** | - | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | |
CultureSure® Y-27632 | - | ✓ | - | - | ✓ | ✓ | ✓ | Powder | |
CultureSure® 10mmol/L Y-27632 Solution, Animal-derived-free |
- | ✓ | ✓ | - | ✓ | ✓ | ✓ | Liquid Sterilized with filter |
* Click to move to the PubMed Central® search results.
** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
*** The manufacturing process and analytical procedure are validated.
Preparation
Solubility Data for Y-27632 dihydrochloride
C14H21N3O・2HCl・H2O = 338.27
Solvent | Conc. mg/mL | Conc. mmol/L | |
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Solubility | Water | 10mg/mL | 29.56 mmol/L |
Ethanol | 10mg/mL | 29.56 mmol/L |
Preparing Stock Solutions
C14H21N3O・2HCl・H2O = 338.27
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
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1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL |
10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL |
50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL |
References
- Watanabe, K., et al.: Nat. Biotechnol., 25, 681 (2007).
A ROCK inhibitor permits survival of dissociated human embryonic stem cells - Masashi, H., et al. : Genes Dev, 1, 36(1-2), 84–102 (2022).
Highly rigid H3.1/H3.2–H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells - Yoshifumi, M., et al. : J Neurosci Res, 98(8), 1575–1587 (2020).).
Zonisamide promotes survival of human‐induced pluripotent stem cell‐derived dopaminergic neurons in the striatum of female rats - Kazutoshi, T., et al. : Cell Rep Methods, 2(2), 100155 (2022).
A stress-reduced passaging technique improves the viability of human pluripotent cells - Yu, K., et al. : eNeuro, 9(2), ENEURO.0442-21 (2022).
Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells - Tomoya, U., et al. : Cell Rep Med, 2(6) 100298 (2021).
A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs - Genki, N., et al. : eLife, 11, e77007 (2022).
Robotic search for optimal cell culture in regenerative medicine - Maobi, Z., et al. : Cell, 8(8), 933 (2019).
Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube - Yu, K., et al. : Front Endocrinol (Lausanne), 13, 941166 (2022).
EpCAM Is a Surface Marker for Enriching Anterior Pituitary Cells From Human Hypothalamic-Pituitary Organoids - Sho, T., et al. : STAR Protoc, 3(2), 101360 (2022).
Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis - Takuro, T., et al. : Commun Biol, 5, 453, (2022).
Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria - Tomoki, Y., et al. : Mol Ther Methods Clin Dev, 10(22), 263–278 (2021).
Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research - Bin, X., et al. : J Neurochem, 153(6), e14949 (2020).
Transplantation of iPS‐derived vascular endothelial cells improves white matter ischemic damage - Hiroyuki, N., et al. : Front Physiol, 10, 818 (2019).
Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias - Yayoi, H., et al. : Front Pharmacol, 12, 680618 (2021).
High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias - Masahiko, A., et al. : Nat Commun, 12, 4507 (2021).
Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia - Maobi, Z., et al. : Biomolecules, 10(9), 1270 (2020).
Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro—Stem Cell of Fallopian Epithelium - Junko, Y., et al. : iScience, 25(7), 104538 (2022).
StemPanTox: A fast and wide-target drug assessment system for tailor-made safety evaluations using personalized iPS cells - Keitaro, K., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells - Koichiro, O., et al. :Sci Rep, 8, 3615 (2018).
Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors - Kengo, K., et al. : Sci Rep, 11, 22476 (2021)
Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage - Hayato, F., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient (PARK4) with SNCA triplication - Yoji, K., et al. : Life Sci Alliance, 4(5), e202000974 (2021).
GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program - Eri, N., et al. : Biochem Biophys Rep, 29, 101195 (2022).
Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP - Kun, H., et al. : Sci Rep, 10, 2558 (2020).
Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells - Yusuke, T., et al. : Int J Mol Sci, 20(6), 1331 (2019).
ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells
Overview / Applications
Outline | This product is for research use only. Do not administer it to human.
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Precautions for Use | Packed on inactive gas |
Property
Appearance | White - pale yellow, crystalline powder - powder |
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Boiling Point | about 85 degrees C |
Specific Rotation | +2.0 - +10.0 degrees (D/20 degrees C)(c=1.0,CH3OH) |
Purity | Bacterial endotoxins : <0.25EU/mg |
Manufacturer Information
Alias
- (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)cyclohexanecarboxamide Dihydrochloride Monohydrate
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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