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Serum-free medium for human pluripotent stem cells

StemSure® hPSC MediumΔ

This product is a serum-free liquid media suitable for maintaining human pluripotent stem cells (hPSCs), human ES cells and human iPS cels in the absence of feeder cells. The raw material of this media is not used components containing any animal-derived materials. Moreover, this media does not contain any albumins (such as BSA or HSA), thereby it can culture hPSCs stably without lot difference. Please note that this medium does not contain basic fibroblast growth factor (bFGF).

Features

  • Feeder-free, serum-free media for culturing hPSCs.
  • Quality control testing of each lot is performed using the human iPS cell 201B7 strain.
  • Animal component-free.
  • Albumin-free, low-protein media.
  • Compatible with various culture vessel surface coating reagents, such as Matrigel®, iMatrix-511 and vitronectin.
  • Compatible with various cell dissociation reagents, such as Accutase and TrypLE Select.
  • Enables single-cell passaging by adding Y-27632 when passage.

Quality Control Testing

  • Cell proliferation assay (using the human iPS cell 201B7 strain)
  • Alkaline phosphatase staining (using the human iPS cell 201B7 strain)
  • Sterility test
  • pH
  • Osmolality
  • Endotoxin test
  • Mycoplasma negative test

Usage Notes

This product does not contain basic fibroblast growth factor (bFGF).
Store frozen at –20°C. After thawed, store at 2-10°C for up to one week. Do not refreeze.

Culture of human iPS Cells 201B7 Strain

Human iPS cells 201B7 strain were first cultured in BSA-containing, feeder-free media manufactured by Competitor A. Subsequently, these cells (passage 0) were transferred to StemSure® hPSC MediumΔ (passage 1) and cultured successively. During subculture, we analyzed their morphology, population doubling levels, and undifferentiated state. The results are shown below.

  • Cell Morphology

    Human iPS cells 201B7 strain were first cultured in BSA-containing, feeder-free media manufactured by Competitor A. Subsequently, these cells (passage 0) were transferred to StemSure hPSC MediumΔ (passage 1) and cultured successively. During subculture, we analyzed their morphology, population doubling levels, and undifferentiated state. The results are shown below.

    00556_img01.jpg
  • Population Doubling Level

    Compared with cells maintained in Competitor A’s medium, human iPS cells transferred to StemSure® hPSC Medium Δ grew faster during the culture period ranging from passage 1 (at the time of cell transfer) to passage 5, and even after.

    00556_img02.jpg

    < Media Compsition >
    StemSure® hPSC MediumΔ + 35ng/ml bFGF
    < Seeding Density >
    1×105cells/well(cultured in 6-well plate)

Maintenance of Undifferentiated State

Cultured in StemSure® hPSC MediumΔ (passage 5) and checked expression of the undifferentiated markers (Nanog, Oct3/4, Tra-1-60, SSEA-4, and BC2LCN).
※BC2LCN is a recombinant lectin with high affinity for glycans that exists on the surface of hPSCs.

00556_img03.jpg

<Data provided by Dr. Yasuko Onuma and Dr. Yuzuru Ito of the Research Center for Stem Cell Engineering at the National Institute of Advanced Industrial Science and Technology.>


 

Culture of human ES Cells WA01 Strain

Human ES Cells WA01 strain were first cultured in BSA-containing, feeder-free media manufactured by Competitor A. Subsequently, these cells (passage 0) were transferred to StemSure hPSC Medium  (passage 1) and cultured successively. During subculture, we analyzed their morphology, population doubling levels, and undifferentiated state. The results are shown below.

  • Cell Morphology

    When cultured in Competitor A’s media, cells of differentiated morphology appeared after passage 2. In contrast, human ES cells cultured in StemSure® hPSC Medium Δ did not show any morphological changes. This indicates that StemSure hPSC MediumΔ can maintain the undifferentiated state of human ES cells.

    00556_img05.jpg
  • Population Doubling Level

    There was no difference in cell proliferation ability compared to the Competitor A’ media from passage 1 after transfer to StemSure® hPSC MediumΔ until passage 5.

    00556_img06.jpg

    < Media Composition >
    StemSure® hPSC MediumΔ + 35ng/ml bFGF
    < Seeding Density >
    1×105cells/well(cultured in 6-well plate)

Maintenance of Undifferentiated State

Cultured in StemSure® hPSC MediumΔ (passage 6) and checked expression of the undifferentiated markers(Nanog, Oct3/4, Tra-1-60, SSEA-4, and BC2LCN).

00556_img07.jpg

< Data provided by Dr. Yasuko Onuma and Dr. Yuzuru Ito of the Research Center for Stem Cell Engineering at the National Institute of Advanced Industrial Science and Technology. >

Karyotyping

Karyotype analysis revealed no chromosomal abnormalities in human iPS cells passaged 27 times in StemSure® hPSC MediumΔ.

00556_img08.jpg

< Data provided by Dr. Takumi Miura and Dr, Hidenori Akutsu of the Center for Regenerative Medicine at the National Research Institute for Child Health and Development. >

Differentiation of Human iPS 201B7 Cells into Three Germ Layers

00556_img09.jpg

< Media Composition >
StemSure® D-MEM + StemSure® Serum
Replacement + 2mmol/l L-Glutamine +
0.1mmol/l StemSure® 2-Mercaptoetanol + 1
x Non-essential Amino Acids Solution

Product List

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StemSureR hPSC Medium Δ

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
197-17571
100mL
List Price
73.00 USD

Out of Stock

Distributor
193-17573
100mL x4
List Price
242.00 USD

Out of Stock

Basic fibroblast growth factor, Y-27632

Storage Condition :
Keep at -20 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
060-05383
1mg
Inquire

Out of Stock

Distributor
064-05381
50ug
List Price
514.00 USD

In stock in Japan

Distributor
068-05384
100ug
List Price
880.00 USD

In stock in Japan

CultureSure® Y-27632

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
030-24021
1mg
List Price
165.00 USD

In stock in Japan

Distributor
036-24023
5mg
List Price
220.00 USD

In stock in Japan

Distributor
034-24024
25mg
List Price
800.00 USD

In stock in Japan

Distributor
030-24026
100mg
List Price
2,000.00 USD

In stock in Japan

Y-27632, MF

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
259-00613
5mg
List Price
605.00 USD

In stock in Japan

Distributor
257-00614
25mg
List Price
2,420.00 USD

In stock in Japan

CultureSureR 10mmol/L Y-27632 Solution, Animal-derived-free

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
035-24593
1mL
List Price
500.00 USD

In stock in Japan

Distributor
039-24591
300uL
List Price
200.00 USD

In stock in Japan

StemSure® series

StemSure® D-MEM(High Glucose) with Phenol Red and Sodium Pyruvate Discontinued

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C. (RT)

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
197-16275
500mL
Discontinued
StemSure® Serum Replacement (SSR)

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
191-18375
500mL
List Price
440.00 USD

In stock in Japan

StemSureR 10mmol/L 2-Mercaptoethanol Solution (×100)

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
198-15781
100mL
List Price
87.00 USD

In stock in Japan

StemSureR 50mmol/L Monothioglycerol Solution(×100)

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
195-15791
100mL
List Price
101.00 USD

In stock in Japan

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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