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CultureSure® Y-27632

for Cell Culture
Specification Assay :
98.0+% (HPLC)
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Molecular Formula :
Molecular Weight :
  • Structural Formula
  • Label
  • Packing
Product Number
Package Size
Barcode No
List Price
150.00 USD

In stock

Barcode No
List Price
220.00 USD

In stock

Barcode No
List Price
800.00 USD

In stock

Barcode No
List Price
2,000.00 USD

In stock in Japan


Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Product grade

Product ICHQ7 mycoplasma Endotoxin Viable bacteria Sterility
Y-27632 (GMP grade is coming soon) -
Y-27632, MF - -
CultureSure® Y-27632 - - -
CultureSure® 10mmol/L Y-27632 Solution, Animal-derived-free - -

*Tests for mycoplasma, endotoxin, and viable bacteria are performed for every lot as part of standard tests.
*We have some types of Y-27632 as your requirement.


Solubility Data for Y-27632 dihydrochloride

C14H21N3O・2HCl・H2O = 338.27

Solvent Conc. mg/mL Conc. mmol/L
Solubility Water 10mg/mL 29.56 mmol/L
Ethanol 10mg/mL 29.56 mmol/L

Preparing Stock Solutions

C14H21N3O・2HCl・H2O = 338.27

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mmol/L 2.956 mL 14.781 mL 29.562 mL
5 mmol/L 0.591 mL 2.956 mL 5.912 mL
10 mmol/L 0.296 mL 1.478 mL 2.956 mL
50 mmol/L 0.059 mL 0.296 mL 0.591 mL


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    A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs
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    Robotic search for optimal cell culture in regenerative medicine
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  9. Yu, K., et al. : Front Endocrinol (Lausanne), 13, 941166 (2022).
    EpCAM Is a Surface Marker for Enriching Anterior Pituitary Cells From Human Hypothalamic-Pituitary Organoids
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  12. Tomoki, Y., et al. : Mol Ther Methods Clin Dev, 10(22), 263–278 (2021).
    Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research
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    Transplantation of iPS‐derived vascular endothelial cells improves white matter ischemic damage
  14. Hiroyuki, N., et al. : Front Physiol, 10, 818 (2019).
    Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias
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    ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells

Overview / Applications

Outline Y-27632 is selective and potent ROCK inhibitor. Y-27632 plays various roles including contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of human embryonic stem cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) at cell dispersion, and that it increases the survival rate of hESCs and hiPSCs during cryopreservation.
Precautions for Use Packed on inactive gas


Appearance White - pale yellow, crystalline powder - powder
Boiling Point about 85 degrees C
Specific Rotation +2 - +10 degrees (D/20 degrees C)(c=0.5,CH3OH)
Purity Bacterial endotoxins : <0.25EU/mg

Manufacturer Information


  • (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide Dihydrochloride Monohydrate

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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