For regenerative medicine research

Y-27632

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It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improves viability of the cells after cryopreservation.
We provide four types of Y-27632 products. You can select according to the purpose of uses.

Y-27632 Comparison Chart

ICH-Q7 ISO9001 Sterility
test
Viable
count
test
Animal-
derived-
free
Bacterial
Endotoxin
test
Mycoplasma
test
Powder/
Liquid
References*
Y-27632 (GMP-compliant) - Powder -
Y-27632, MF - - Powder
CultureSure® Y-27632 - - - Powder
CultureSure® 10mmol/L
Y-27632 Solution,
Animal-derived-free
- - Liquid
Sterilized with filter

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Product Overview

Y-27632 (GMP-compliant)

This product is complied with ICH-Q7 (GMP for APIs) and is intended as a raw material for the commercial production of regenerative medicine products. It has been tested for viable count, as well as bacterial endotoxins, mycoplasma contamination, and residual solvent.

  • GMP compliant
  • Subdivision in a clean room equivalent to ISO14644-1 Class 6
  • Reduction of contamination risk through the use of dedicated equipment for manufacturing culture media additives
  • Process validation, analytical validation, and cleaning validation carried out
  • Animal-derived-free

Y-27632, MF

This is an ISO9001-compliant product developed for the commercial manufacturing of regenerative medicine products. It has been tested for viable count, as well as bacterial endotoxins and mycoplasma contamination.
The management and manufacturing standards have been established internally.

  • ISO9001 compliant
  • Reduction of contamination risk through the use of dedicated equipment for manufacturing culture media additives
  • Process validation, analytical validation, and cleaning validation carried out
  • Animal-derived-free

CultureSure® Y-27632

This product was developed for basic research in drug discovery and regenerative medicine. It has been tested for bacterial endotoxins and mycoplasma contamination.

  • ISO9001 compliant
  • Animal-derived-free

CultureSure® 10 mmol/L Y-27632 Solution, Animal-derived-free

This product is a 10 mmol/L aqueous solution of Y-27632. It has been tested for sterility, as well as bacterial endotoxins and mycoplasma contamination. It has been 0.1 μm filter-sterilized and can be added directly to the culture medium.

  • ISO9001 compliant
  • Animal-derived-free

Glossary

GMP

Good Manufacturing Practice (GMP) is a standard for manufacturing and quality control of pharmaceutical drugs, etc., and should be observed by manufacturers of pharmaceutical drugs, etc.
The GMP standards are based on the following three principles:

  1. Minimizing human errors during manufacture
  2. Preventing contamination and quality deterioration of pharmaceutical products
  3. Designing a system for high quality assurance

ICH-Q7 (GMP for APIs)

International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) is an international initiative to create guidelines on pharmaceutical regulations from scientific and technical perspectives by joint efforts of regulatory authorities and representatives of the pharmaceutical industry.

Among these guidelines, ICH-Q7 (GMP for APIs (Active Pharmaceutical Ingredients)) outlines the standard practices for manufacturing management and quality control related to active pharmaceutical ingredients.

ISO9001

ISO stands for the International Organization for Standardization, a non-governmental organization. The standards established by the ISO are referred to as ISO standards. These are internationally agreed-upon criteria established by experts, covering various areas such as product manufacturing, process management, service provision, and material supply.
ISO 9001 is a standard set out for quality management systems. Many companies and organizations are certified to ISO 9001 to ensure that their customers consistently receive good quality products and services.

Reference data of preparation

The powder Y-27632 product has been confirmed to be soluble in water. After dilution, filter sterilize before use. Refer to the protocol at the top of this page for general usage in ES/iPS cell culture.

Amount of solvent when preparing solution

Y-27632 (powder)
1 mg 5 mg 10 mg
Concentration 1 mmol/L 2.956 mL 14.781 mL 29.562 mL
5 mmol/L 0.591 mL 2.956 mL 5.912 mL
10 mmol/L 0.296 mL 1.478 mL 2.956 mL
50 mmol/L 0.059 mL 0.296 mL 0.591 mL

White Paper

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Products listed in the protocol

This product does not contain bFGF (basic Fibroblast Growth Factor).

Please use this product as a dish coating agent as needed.

Cytokine solutions

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.