Y-27632
It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improves viability of the cells after cryopreservation.
Y-27632 Comparison Chart
We provide four types of Y-27632 products. You can select according to the purpose of uses.
| Mycoplasma negative |
Endotoxin test | Animal-derived-free | Viable bacteria |
Sterility | ISO9001 | ICH-Q7 | powder/ liquid |
References* | |
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| Y-27632 (GMP compliant)** |  |
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powder | - |
| Y-27632, MF*** | |
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- | powder | |
| CultureSure® 10mmol/L Y-27632 Solution, Animal-derived-free |
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- | liquid Sterilized with filter |
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| CultureSure® Y-27632 | |
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- | - | |
- | powder |
* Click to move to the PubMed Central® search results.
** This product complies with ICH-Q7 (GMP for API) guidelines as a raw material for commercial production of regenerative medicine products. This is manufactured under properly controlled facilities.
*** The manufacturing process and analytical procedure are validated.
Reference data of preparation
The powder Y-27632 product has been confirmed to be soluble in water. After dilution, filter sterilize before use. Refer to the protocol at the top of this page for general usage in ES/iPS cell culture.
Amount of solvent when preparing solution
| Y-27632 (powder) | ||||
|---|---|---|---|---|
| 1 mg | 5 mg | 10 mg | ||
| Concentration | 1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
| 5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL | |
| 10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL | |
| 50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL | |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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