For regenerative medicine research



It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improves viability of the cells after cryopreservation.


Y-27632 Comparison Chart

We provide four types of Y-27632 products. You can select according to the purpose of uses.

Endotoxin test Animal-derived-free Viable
Sterility ISO9001 ICH-Q7 powder/
Y-27632 (GMP compliant)** - powder -
Y-27632, MF*** - - powder
CultureSure® 10mmol/L
Y-27632 Solution,
- - liquid
Sterilized with filter
CultureSure® Y-27632 - - - powder

* Click to move to the PubMed Central® search results.
** This product complies with ICH-Q7 (GMP for API) guidelines as a raw material for commercial production of regenerative medicine products. This is manufactured under properly controlled facilities.
*** The manufacturing process and analytical procedure are validated.

Reference data of preparation

The powder Y-27632 product has been confirmed to be soluble in water. After dilution, filter sterilize before use. Refer to the protocol at the top of this page for general usage in ES/iPS cell culture.

Amount of solvent when preparing solution

Y-27632 (powder)
1 mg 5 mg 10 mg
Concentration 1 mmol/L 2.956 mL 14.781 mL 29.562 mL
5 mmol/L 0.591 mL 2.956 mL 5.912 mL
10 mmol/L 0.296 mL 1.478 mL 2.956 mL
50 mmol/L 0.059 mL 0.296 mL 0.591 mL

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Products listed in the protocol

This product does not contain bFGF (basic Fibroblast Growth Factor).

Please use this product as a dish coating agent as needed.

Cytokine solutions

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.


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