Y-27632
It is a selective and potent inhibitor of ROCK (Rho-associated coiled-coil forming kinase/Rho binding kinase).Y-27632 has a variety of activities including vascular smooth muscle contraction through the ROCK signaling (Ki = 140 nmol/L p160ROCK). This product has been reported to suppress death of human ES cells and human iPS cells at the time of cell dispersion, and also improves viability of the cells after cryopreservation.
We provide four types of Y-27632 products. You can select according to the purpose of uses.
Y-27632 Comparison Chart
| ICH-Q7 | ISO9001 | Sterility test |
Viable count test |
Animal- derived- free |
Bacterial Endotoxin test |
Mycoplasma test |
Powder/ Liquid |
References* | |
|---|---|---|---|---|---|---|---|---|---|
| Y-27632 (GMP-compliant) | ✓ | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | - |
| Y-27632, MF | - | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | |
| CultureSure® Y-27632 | - | ✓ | - | - | ✓ | ✓ | ✓ | Powder | |
| CultureSure® 10mmol/L Y-27632 Solution, Animal-derived-free |
- | ✓ | ✓ | - | ✓ | ✓ | ✓ | Liquid Sterilized with filter |
* Click to move to the PubMed Central® search results.* Click to move to the PubMed Central® search results.
Product Overview
Y-27632 (GMP-compliant)
This product is complied with ICH-Q7 (GMP for APIs) and is intended as a raw material for the commercial production of regenerative medicine products. It has been tested for viable count, as well as bacterial endotoxins, mycoplasma contamination, and residual solvent.
- GMP compliant
- Subdivision in a clean room equivalent to ISO14644-1 Class 6
- Reduction of contamination risk through the use of dedicated equipment for manufacturing culture media additives
- Process validation, analytical validation, and cleaning validation carried out
- Animal-derived-free
Y-27632, MF
This is an ISO9001-compliant product developed for the commercial manufacturing of regenerative medicine products. It has been tested for viable count, as well as bacterial endotoxins and mycoplasma contamination.
The management and manufacturing standards have been established internally.
- ISO9001 compliant
- Reduction of contamination risk through the use of dedicated equipment for manufacturing culture media additives
- Process validation, analytical validation, and cleaning validation carried out
- Animal-derived-free
CultureSure® Y-27632
This product was developed for basic research in drug discovery and regenerative medicine. It has been tested for bacterial endotoxins and mycoplasma contamination.
- ISO9001 compliant
- Animal-derived-free
CultureSure® 10 mmol/L Y-27632 Solution, Animal-derived-free
This product is a 10 mmol/L aqueous solution of CultureSure® Y-27632. It has been tested for sterility, as well as bacterial endotoxins and mycoplasma contamination. It has been 0.1 μm filter-sterilized and can be added directly to the culture medium.
- ISO9001 compliant
- Animal-derived-free
Glossary
GMP
Good Manufacturing Practice (GMP) is a standard for manufacturing and quality control of pharmaceutical drugs, etc., and should be observed by manufacturers of pharmaceutical drugs, etc.
The GMP standards are based on the following three principles:
- Minimizing human errors during manufacture
- Preventing contamination and quality deterioration of pharmaceutical products
- Designing a system for high quality assurance
ICH-Q7 (GMP for APIs)
International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) is an international initiative to create guidelines on pharmaceutical regulations from scientific and technical perspectives by joint efforts of regulatory authorities and representatives of the pharmaceutical industry.
Among these guidelines, ICH-Q7 (GMP for APIs (Active Pharmaceutical Ingredients)) outlines the standard practices for manufacturing management and quality control related to active pharmaceutical ingredients.
ISO9001
ISO stands for the International Organization for Standardization, a non-governmental organization. The standards established by the ISO are referred to as ISO standards. These are internationally agreed-upon criteria established by experts, covering various areas such as product manufacturing, process management, service provision, and material supply.
ISO 9001 is a standard set out for quality management systems. Many companies and organizations are certified to ISO 9001 to ensure that their customers consistently receive good quality products and services.
Reference data of preparation
The powder Y-27632 product has been confirmed to be soluble in water. After dilution, filter sterilize before use. Refer to the protocol at the top of this page for general usage in ES/iPS cell culture.
Amount of solvent when preparing solution
| Y-27632 (powder) | ||||
|---|---|---|---|---|
| 1 mg | 5 mg | 10 mg | ||
| Concentration | 1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
| 5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL | |
| 10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL | |
| 50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL | |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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