Valproic Acid

Cell Biological ResearchFor Research on Cell Cycle and TranscriptionHistone Deacetylase (HDAC) InhibitorsHistone deacetylase (HDAC) plays a central role in chromatin structure formation associated with the nuclear distribution of DNA. There are presently 17 known types of this enzyme in mammals, which are classifiedinto 3 classes (see table below). Also, HDAC Class III has been reported to be associated with regulation of aging and life span.

• Mammalian HDAC Class I: Localized in the nucleus. Is similar to RPD3 enzyme. [Types : HDAC1-3, 8]
• Mammalian HDAC Class II: Localized in the nucleus and cytoplasm and shuttles between the two. Similar to HDA1 enzyme. [Types: HDAC4-7, 9, 10]
• Mammalian HDAC Class III: NAD-dependent enzyme. Localized in the nucleus and cytoplasm. Also called Sirtuin and is similar to SIR2 enzyme. [Types : Sirt 1-7]

HDAC inhibitors show connections with cell division cycles and differentiation, as well as with antitumor activity and apoptosis-inducing activity through the inhibition of the deacetylating activity of HDAC. They can be used for studies on cellular functions involving histone deacetylase.

[Related Products]

• Piceatannol (Wako Cat.#169-21661 (10 mg)) Activator of HDAC Class III and inhibitor of kinase Syk.


• Sirtinol (Wako Cat.#197-13671 (5 mg)) Inhibitor of HDAC Class III. Cell-permeable.


• Trichostatin A (Wako Cat.#203-17561 (1 mg); #209-17563 (5 mg)) Potent and reversible inhibitor of HDAC Class I and II.


• Valproic Acid (Wako Cat.#227-01071 (5 g); #225-01072 (25 g)) Inhibitor of HDAC1.

• Maintain ES & iPs cells in an undifferentiated state


• Improve reprogramming efficiency

Enhances efficiency of iPS cell induction by Oct4, Klf4 and Sox2.

Reference:

1. Huangfu, D., et al.: Nature Biotechnol., 25, 1269 (2008)