Culture medium additives for regenerative medicine

MF-registered Small Molecules

The products are registered in the Drug Master File (MF) in Japan as additives for culture medium.
We ensure high stability and quality of the products by validating the manufacturing and analytical processes and implementing change control strategies.

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Y-27632, MF

ROCK inhibitor

Y-27632 improves the viability and cloning efficiency of human ES/ iPS cells after cryopreservation. Furthermore, Y-27632 can be used with EGF, CHIR99021, A-83-01, and SB431542 to establish Trophoblast stem (TS) cells from human blastocysts.

<Product Description>

  • Appearance: white to pale yellow, crystalline powder to powder
  • Assay (HPLC): min. 98.0%
  • Solubility: soluble in water
  • Specific rotation [α]D20(c=1.0,CH3OH) :+2.0°~+10.0°
  • Endotoxin ※: less than 0.25 EU/mg
  • Tested negative for mycoplasma ※
  • Tested for viable bacteria ※
  • CAS RN®. 331752-47-7
  • C14H21N3O・2HCl・H2O=338.27
  • [Registration number: 227MF40013]
References
  1. Uehata, M., et al. : Nature, 389, 990 (1997).
  2. Sakamoto, K., et al. : J. Pharmacol. Sci., 92, 56 (2003).
  3. Nishimaru, K., et al. : J. Pharmacol. Sci., 92, 424 (2003).
  4. Watanabe, K., et al. : Nat. Biotechnol., 25, 681 (2007).
  5. Martin-Ibanez, R., et al. : Hum. Reprod., 23, 2744 (2008).
  6. Claassen, DA., et al. : Mol. Reprod. Dev., 76, 722 (2009).
  7. Kawamata, M., et al. : Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
  8. Ito, H., et al. : Liver Int., 32, 592 (2012).
  9. Katsuda,T., et al. : Cell Stem Cell, 20, 41 (2017).
  10. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
  11. Ogawa, K., et al. : Sci. Rep., 8, 3615 (2018).
  12. Ayabe, H., et al. : Stem Cell Reports., 11, 306 (2018).

A-83-01, MF

Inhibitor of ALK4, ALK5, and ALK7

A-83-01 enables long-term and consistent culture of rat and canine iPS cells in a state maintaining the undifferentiated state.. Furthermore, the use of A-83-01 with Y-27632 and CHIR99021 induces reprogramming of rat and mouse mature hepatocytes into liver progenitors that can be cultured stably in vitro

<Product Description>

  • Appearance: white to yellow, crystalline powder to powder or mass
  • Assay (HPLC): min. 98.0%
  • Solubility: soluble in DMSO
  • Endotoxin ※: less than 0.01 EU/mg
  • Tested negative for mycoplasma ※
  • Tested for viable bacteria ※
  • CAS RN®. 909910-43-6
  • C25H19N5S=421.52
  • [Resistration number: 229MF40005]
References
  1. Li, W., et al. : Cell Stem Cell, 4, 16 (2009).
  2. Katsuda ,T., et al. : Cell Stem Cell, 20, 41 (2017).
  3. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

SB431542, MF

Inhibitor of ALK4, ALK5, and ALK7

SB431542 promotes proliferation, differentiation and sheet formation of ES cells-derived endothelial cells. It also improves reprogramming efficiency and speed when used with PD0325901 and thiazovivin. It has also been reported that cells induced by SB43154, CHIR99021, and dorsomorphin have significantly improved differentiation efficiency and speed.

<Product Description>

  • Appearance: white to yellowish brown, crystalline powder to powder
  • Assay(HPLC): min. 98.0%
  • Solubility: soluble in DMSO
  • Endotoxin ※: less than 0.05 EU/mg
  • Tested negative for mycoplasma ※
  • Tested for viable bacteria ※
  • CAS RN®. 301836-41-9
  • C22H16N4O3=384.39
  • [Resistration number: 228MF40016]
References
  1. Ogawa, K., et al. : J. Cell. Sci.,120, 55 (2007).
  2. Lin T., et al. : Nat. Methods, 6, 805 (2009).
  3. Fujimori, K., et al. : Stem Cell Reports., 9, 1 (2017).
  4. Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).

Note

  • Tests for mycoplasma, endotoxin, and viable bacteria are performed for every lot as part of standard tests; however, the results are not required for MF registration.
  • Registration in the Drug Master File (MF) does not indicate that the product was tested for its quality and safety by the Ministry of Health, Labor and Welfare (Pharmaceuticals and Medical Devices Agency).

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※The registration in the Drug Master File in Japan (Master File) does not mean that the confirmation or evaluation of the quality and safety have been performed by the Ministry of Health, Labour and Welfare (Pharmaceuticals and Medical Devices Agency).

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Regenerative medicine product material qualification consulted product

Product content may differ from the actual image due to minor specification changes etc.

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