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Digitonin (Water soluble)

for Biochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.
CAS RN® :
11024-24-1
Molecular Formula :
C56H92O29
Molecular Weight :
1229.31
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
043-21371
Barcode No
4987481253841
1g
Inquire

In stock

Distributor
047-21374
Barcode No
4987481376595
5g
Inquire

In stock

Distributor
043-21376
Barcode No
4987481376601
500mg
List Price
539.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Overview

Digitonin is a spirostan saponin obtained from the seeds and leaves of digitalis (D. purpurea, D. lanata). A mild nonionic detergent, digitonin is used in a wide range of applications, including solubilization of membrane proteins, permeabilization of cellular membranes, isolation of mitochondria and quantitation of cholesterol.

Water Solubility and Aqueous Solution Stability

The water solubility and aqueous solution stability of Digitonin (Water Soluble) were compared to that of a competitor’s equivalent product. Digitonin solutions (10 w/v%) were prepared at room temperature or at 60℃, and stored at 4℃ for 15 days, prior to optical density (600nm) measurements.

  • 01637_img02.png
  • ■Compared products:
    - Wako Digitonin (Water soluble)
    - Company A’s water-soluble Digitonin
    ■Wako Digitonin lot no.: PTF7501
    ■Tested solubility: 10 w/v%
    ■Aqueous solution preparation procedure:
    1. Room temperature, mixing for 5 minutes.
    2. Incubation at 60℃ for 5 minutes.
    ■Aqueous solution storage temperature: 4℃.

    01637_img03.png

 

Condition 1: Dissolved at Room temperature → Stored at 4℃ for 15 days

Wako Digitonin (Water Soluble) :
 No notable increase of OD value up to Day 10.
Company A’s Water Soluble Digitonin:
 Increase of OD value from Day 1.

Condition 2: Incubated at 60℃ for 5 minutes → Stored at 4℃ for 15days

Wako Digitonin (Water Soluble) :
 No precipitation observed by the end of the experiment (Day 15).
Company A’s Water Soluble Digitonin:
 Precipitation observed on Day 13.

 

Solubility and stability tests were performed on a 10 w/v% aqueous solution made from a typical lot of Digitonin. Note that 10 w/v% is much higher than the concentration adopted as quality criterion. Wako guarantees 4 w/v% solubility for every lot of Digitonin.

Overview / Applications

Property

Appearance White - nearly white, powder
Specific Rotation -45 - -50 degrees (D/20 degrees C)(c=5,CH3COOH)
(after drying)

Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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