Column for HPLC
High performance liquid chromatography (HPLC) is mainly used for fractionation and analysis, and separation of the analyte is performed by the column.
Wakopak® series is a column for HPLC and UHPLC using a packing agent based on a fully porous high-purity silica gel. FUJIFILM Wako offers columns for various separation modes, including application-specific columns for analysis (pesticides, amino acids, aldehyde-ketones, surfactants, etc.) as well as polymer columns, optical isomer separation columns, SFC columns, and core-shell columns.
Product Line-up
- Silica Gel Packing Agent for HPLC Columns (Unmodified, C18 Modified)
- Quality Inspection of Wakopak® Silica Gel Packing Agents
- GMP Analytical Validation Support
-
- Wakopak® Wakosil®-Ⅱ 5C22
- Wakopak® Wakosil® 5Ph
- Wakopak® Wakosil®-II C8 Series (5C8 HG, RS, 3C8 RS)
- Wakopak® Wakosil®-Ⅱ 5C18 HG, RS, AR
- Wakopak® Wakosil®-Ⅱ 5C18, 5C18-100
- Wakopak® Wakosil®-Ⅱ 3C18 HG, RS, AR
- Wakopak® Wakosil® C18 Series
- Wakopak® Ultra Series (C18)
- HPLC Column for Pesticide Residue Analysis "Wakopak® Wakosil® Agri-9"
- Wakopak® Navi Series (C18 / C22 / C30)
- Wakopak® Handy ODS, Wakopak® eco-ODS
- Wakopak® Fluofix®-II 120E
More Information
How to Select HPLC Columns
The separation of HPLC differs depending on the combination of column and mobile phase. There are various factors for selection of mobile phase such as type of solvent, mobile phase composition ratio and pH, and the corresponding column selection is necessary. In addition, column length, inside diameter and particle size are also important factors.
The following table summarizes the information on Wakopak® series (packing agent, particle size, column pressure, pH range, etc.). Please use it when selecting columns.
Select from USP L Column Listing
The USP classifies the packing agents used for HPLC by attribute. The correspondence between the columns of FUJIFILM Wako and USP L number is summarized below. Please use it when selecting columns.
| USP L## | Functional group | Definitions | Wakopak® |
|---|---|---|---|
| L1 | C18 | Octadecylsilane chemically bonded to porous or non-porous silica or ceramic microparticles, 1.5 to 10 µm in diameter. |
Combi ODS |
| Combi ODS fast | |||
| eco-ODS | |||
| Handy ODS | |||
| Navi C18-5 | |||
| Ultra C18-2 | |||
| Ultra C18-3 | |||
| Ultra C18-5 | |||
| Wakosil-Ⅱ 3C18 AR | |||
| Wakosil-Ⅱ 3C18 HG | |||
| Wakosil-Ⅱ 3C18 RS | |||
| Wakosil 5C18 | |||
| Wakosil-Ⅱ 5C18 | |||
| Wakosil-Ⅱ 5C18-100 | |||
| Wakosil 5C18-200 | |||
| Wakosil 5C18-200N | |||
| Wakosil 5C18-200T | |||
| Wakosil 5C18AR | |||
| Wakosil-Ⅱ 5C18 AR | |||
| Wakosil-Ⅱ 5C18 AR Prep | |||
| Wakosil-Ⅱ 5C18 HG | |||
| Wakosil-Ⅱ 5C18 HG Prep | |||
| Wakosil 5C18N | |||
| Wakosil-Ⅱ 5C18 RS | |||
| Wakosil-Ⅱ 5C18 RS Prep | |||
| Wakosil 5C18T | |||
| Wakosil 7C18 | |||
| Wakosil 10C18 | |||
| Wakosil 10C18-200 | |||
| Wakosil-Cu | |||
| Wakosil-PAHs | |||
| L3 | SIL | Porous silica particles, 1.5 to 10 µm in diameter. | Wakosil-Ⅱ 5SIL-AQ |
| Wakosil 5SIL | |||
| Wakosil 5SIL-120 | |||
| Wakosil-Ⅱ 5SIL Prep | |||
| Wakosil 7SIL-120 | |||
| Wakosil 10SIL | |||
| L7 | C8 | Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10 µm in diameter. |
Wakosil-Ⅱ 3C8 RS |
| Wakosil 5C8 | |||
| Wakosil-Ⅱ 5C8 HG | |||
| Wakosil-Ⅱ 5C8 RS | |||
| L8 | NH2 | Aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter. |
Wakosil 5NH2 |
| L10 | CN | Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter. |
Combi CN |
| Wakosil 5CN | |||
| Wakosil-Ⅱ 5CN | |||
| L11 | Ph | Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter. |
Wakosil 5Ph |
| L13 | C1 | Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter. |
Wakosil 5TMS |
| L20 | Diol | Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to 10 µm in diameter. |
Wakosil 5Diol-60 |
| Wakosil 5Diol-120 | |||
| Wakosil 5Diol-200 | |||
| L26 | C4 | Butylsilane chemically bonded to totally porous silica particles, 1.5 to 10 µm in diameter. | Wakosil 5C4 |
| Wakosil 5C4-200 | |||
| L33 | Diol | Packing having the capacity to separate proteins of 4 to 500 kDa. It is spherical, silica-based and processed to provide pH stability. |
Wakosil 5Diol-60 |
| Wakosil 5Diol-120 | |||
| Wakosil 5Diol-200 | |||
| Wakosil 5Diol-300 | |||
| L59 | Diol | Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7,000 kDa. Thepacking is spherical 1.5-10 µm, silica or hybrid packing with a hydrophilic coating. |
Wakosil 5Diol-60 |
| Wakosil 5Diol-120 | |||
| Wakosil 5Diol-200 | |||
| Wakosil 5Diol-300 | |||
| L62 | C30 | C30 silane bonded phase on a fully porous spherical silica, 3-15 µm in diameter. | Navi C30-5 |
| L118 | C18 | Aqueous polymerized C18 groups on silica particles, 1.2 to 5 µm in diameter. | Wakosil-PAHs |
| L## | Polyfluoroalkyl | (Ethylhexyl triazone, FluoFix)-Fluorocarbon chains chemically bonded to 5 µm spherical silica particles. |
Fluofix-Ⅱ 120E |
Product Line-up
- Silica Gel Packing Agent for HPLC Columns (Unmodified, C18 Modified)
- Quality Inspection of Wakopak® Silica Gel Packing Agents
- GMP Analytical Validation Support
-
- Wakopak® Wakosil®-Ⅱ 5C22
- Wakopak® Wakosil® 5Ph
- Wakopak® Wakosil®-II C8 Series (5C8 HG, RS, 3C8 RS)
- Wakopak® Wakosil®-Ⅱ 5C18 HG, RS, AR
- Wakopak® Wakosil®-Ⅱ 5C18, 5C18-100
- Wakopak® Wakosil®-Ⅱ 3C18 HG, RS, AR
- Wakopak® Wakosil® C18 Series
- Wakopak® Ultra Series (C18)
- HPLC Column for Pesticide Residue Analysis "Wakopak® Wakosil® Agri-9"
- Wakopak® Navi Series (C18 / C22 / C30)
- Wakopak® Handy ODS, Wakopak® eco-ODS
- Wakopak® Fluofix®-II 120E
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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