Reverse Phase Chromatography (C1 (TMS), C4, C8)

Liquid chromatography (LC) is a commonly used method for separating mixtures for qualitative and quantitative analysis, utilizing the difference in polarity between the analyte in the mobile phase and the packing agent.
In LC, when the polarity of the packing agent is lower than that of the mobile phase, it is called reverse phase chromatography; conversely, when the polarity of the packing agent is higher than that of the mobile phase, it is called normal phase chromatography.
The packing agent (column) and mobile phase (solvent) in LC greatly affect the separation of analytes, so it is important to select the column and solvent to match the analyte.

In reverse phase chromatography, columns packed with octadecyl group (C18, ODS) modified silica gels are commonly used, but if you want to elute strongly retained compounds quickly or if the ratio of organic solvents in the mobile phase cannot be increased, columns with low carbon content and short alkyl chain lengths like trimethylsilyl (C1, TMS), butyl (C4), or octyl (C8) groups are more effective than those with ODS.

Fujifilm Wako offers the Wakopak^® Wakosil^® series of columns packed with TMS, C4, and C8 modified silica gels. The following product pages provide information on each column and its application data.

Product Line-up

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Wakopak^® Series Information (Packing Agent, Particle Size, Pressure, pH Range, etc.)

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Product Lineup

Series name	Functional group	Features
Wakopak^®Wakosil^®TMS	Trimethylsilyl group (C1, TMS)	Lowest carbon content among our HPLC columns. Effective for rapid analysis of hydrophobic compounds. Can be used for samples of a wide range of polarity. Can be used for analyzing the composition of liposomes¹⁾.
Wakopak^®Wakosil^®C4	Butyl group (C4)	Low carbon content compared to common ODS and C8 columns. Shorter analysis time for hydrophobic analytes than C8 columns.
Wakopak^® Wakosil^®/ Wakosil^®-ll C8	Octyl group (C8)	Low carbon content compared to common ODS columns. Allows shorter analysis time for hydrophobic analytes. Wakosil^®-II is available in series (HG, RS) with features such as high theoretical plates and high end capping rate.

1. Shimizu, Y. et al.: J. of Chromatog. B, 754, 127 (2000).

Comparison of Elution Times of Hydrophobic Analytes by Carbon Content (C1, C4, C8, C18)

Elution Time

ODS (C18) column
(Carbon content: 20%*)
*Carbon content of Wakosil^® 5C18

C8 column (carbon content: 12%*)
*Carbon content of Wakosil^® 5C8

C4 column (carbon content: 8%*)
*Carbon content of Wakosil^® 5C4

TMS column (carbon content: 4%*)
* Carbon content of Wakosil^® 5TMS

Please refer to here for analytes to be measured and analysis conditions.

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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