Polymer Gel Wakopak® Wakobeads® -G Series

Wakopak® Wakobeads® (WB)-G series is an HPLC column filled with a polyacrylic acid esters porous spherical gel and was designed on the basis of aqueous GPC separation.

This column is designed for aqueous GPC analysis but is packed uniformly in high density with a chemically stable and less swollen / contracted gel. So it can be used in mixture with an organic solvent or 100% organic solvent. The recommended organic solvents are methanol, acetone, chloroform, dioxane, tetrahydrofuran, N,N-dimethylformamide, and dimethyl sulfoxide.

Four types by each exclusion limit of molecular weight are available. Cartridge precolumn WB-F (1 holder + 4.0 mm x 10 mm cartridge) and replacement cartridge column WB-FC (4 pieces per cartridge) are available for protection of the analytical column.


Calibration Curve



Physical Properties and Size

Type of Column (Packing Agent) WB-G-20 WB-G-30 WB-G-40 WB-G-50
Column Size 7.8 x 300 mm *1
Exclusion Limit of Molecular Weight*2 6 x 103 5 x 104 4 x 105 2 x 106
Number of Theoretical Plates 8,000 7,000 8,000 8,000
Upper Limit of Column Pressure 30 kg/cm2 or less
Upper Limit of Flow Rate 1.5 mL/min.
Optimal Flow Rate 1.0 mL/min.

Solvent Type / Exclusion Limit

Type of Column (Packing Agent) WB-G-20 WB-G-30 WB-G-40 WB-G-50
Water-Pullulan, PEG 6 x 103 5 x 104 4 x 105 2 x 106
THF-Polystyrene 2.5 x 103 5.5 x 104 5.5 x 105 2 x 106
CHCl3-Polystyrene 1 x 103 5.5 x 104 4 x 105 2 x 106
DMF-Polystyrene 3.5 x 103 6 x 104 4 x 105 2 x 106

*1: Column type is Waters type
*2: Measured value with water 100% mobile phase

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