Wakopak^® Wakosil^®-Ⅱ 5C18 HG, RS, AR

• High-purity silica gel is used and is optimal for the analysis of metal coordination compounds
• Endcapping treatment is optimal for sharp separation of basic compounds
• High resolution / high number of theoretical plates

• Metal Coordination Compounds
  Basic Compounds

Certificate of Analysis

Analysis of Scopolamine and Atropine

• 

• Sample Column size Eluent Flow Rate Detection

  ：1) Scopolamine HBr 　2) Atropine Sulfate 　3) Brucine (IS) ：4.6 mm x 150 mm ：CH3CN/50 mM KH2PO4, 1% Triethylamine (pH 3.5 ← H3PO4) 　=10/90 (v/v) ：1.0 mL/min. ：UV 210 nm

Features

Wakopak^® Wakosil^®-II 5C18 HG uses a packing agent obtained by converting highly pure silica gel to monomeric ODS and endcapping. It is suitable for the analysis of basic compounds / metal coordination compounds. Particularly, this product features a high number of theoretical plates. The column efficiency is as high as approximately 15,000 plates at a column size of 4.6 mm × 150 mm, and good results, which have not been obtained with conventional columns, are obtained.

Analysis of Metal Coordination Compounds

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 150 mm) ：CH3CN/20 mM K2HPO4 (pH 7.6) = 40/60 (v/v) ：1.0 mL/min. at 30℃ ：UV 254 nm 0.16 AUFS

Analysis of Alkylphenols

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 250 mm) ：CH3OH/H2O = 60/40 (v/v) ：1.0 mL/min. at 40℃ ：UV 225 nm

Analysis of 13 Type of Catecholamine Metabolites

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 HG (4.6 mm x 150 mm) ：CH3OH/0.1M KH2PO4 , H3PO4 (pH3.9) = 10/90 (v/v) containg SOS 240 mg/L ：1.0 mL/min. at 35℃ ：ECD + 800 mV, 50 nAFS

Features

By improving ODS treatment and endcapping, high resolution / high retention capability are also achieved in aqueous eluents, and in particular, retention is in a balanced manner in mixed samples with a wide range of polarities. In addition, thorough endcapping of the residual silanol groups enables sharp analysis of basic compounds without tailing.

Procainamide and Phenol Analysis

• 

• Sample Column Eluent Flow Rate Detection

  ：1) Procainamide Hydrochloride 　2) Phenol ：Wakopak® Wakosil®-Ⅱ 5C18 RS (4.6 mm x 150 mm) ：CH3OH/20 mM K2HPO4, (pH 7.6) = 40/60 (v/v) ：1.0 mL/min. at 30℃ ：UV 254 nm

Analysis of Water-soluble Vitamins

• 

• Sample Column Eluent Flow Rate Detection

  ：1) L(＋)-Ascorbic acid（V.C） 　2) Nicotinic acid 　3) Nicotinamide 　4) Pyridoxine・HCl (V.B1) 　5) Caffein 　6) Thiamine (V.B1) 　7) d-Biotin (B.H) 　8) Riboflavin (V.B2) ：Wakopak® Wakosil®-Ⅱ 5C18 RS (4.6 mm x 150 mm) ：CH3OH/0.1% H3PO4 , 5 mM SHS* = 10/90 (v/v) ：1.0 mL/min. at 40℃ ：UV 210 nm. 0.16 AUFS *: 1-Hexanesulfonic Acid Sodium Salt

Features

Wakopak^® Wakosil^®-II 5C18 AR uses a polymeric ODS packing agent. This packing agent is a very durable, and even when the mobile phase is acidic (pH1.4), ODS is not readily hydrolyzed. It is useful for the separation of peptides and structural isomers based on the characteristics of polymeric ODS. In addition, separation characteristics may differ from those of typical monomeric ODS silica gel and may show a very specific behavior.

Analysis of Endothelins (ETs)

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm) ：A) CH3CN/0.1%TFA = 20/80 (v/v) 　B) CH3CN/0.1%TFA = 50/50 (v/v) 　0-15 min. B conc. 30-70% ：1.0 mL/min. at 40℃ ：UV 220 nm

Analysis of Enkephalins

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm) ：A) H2O 0.1% TFA 　B) CH3CH 0.1% TFA 　0→25 min. B conc. 10-60% 　25→30 min. B conc. 60% ：1.0 mL/min. at 40℃ ：UV 220 nm 0.256 AUFS.

Analysis of Polycyclic Aromatic Hydrocarbons

• 

• Column Eluent Flow Rate Detection

  ：Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 250 mm) ：A) CH3CN B)H2O 　0→3 min. A conc. 50% 　3→10 min. A conc. 50→100% ：1.5 mL/min. at R.T. ：UV 254 nm 0.128 AUFS.

Resistance Data

	Acid resistance data	Column Eluent Flow Rate Detection ：Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm) ：CH3CN/H2O = 60/40 (v/v) ：1.0 mL/min. at R.T. ：UV 254 nm 　 After passing 1.0% trifluoroacetic acid solution (pH1.4) to each column, allow to stand in a thermostat bath at 60°C, and determine the retention factor K (as the uracil peak at t0) of the naphthalene peak every 14 hours.
	Alkaline resistance data	Column Eluent Flow Rate Detection ：Wakopak® Wakosil®-Ⅱ 5C18 AR (4.6 mm x 150 mm) ：40% CH3CN in 20 mM Na2HPO4 (pH9.4) ：1.0 mL/min. at 40℃ ：UV 254 nm 　 Passing the mobile phase continuously to each packed column at 40°C. Determine the number of theoretical plates N from the N,N-dimethylaniline peak at the specified time.