Wakopak® Wakosil® 5C8, 5C4, 5C4-200

Wakopak® Wakosil® 5C8, 5C4, 5C4-200 is a HPLC column packed with silica gel which is chemically modified with octyl groups or butyl groups.
Wakopak® Wakosil® 5C4-200 is often used for samples of moderate to high polarity. It is also effective for ion-pair chromatography, showing high recovery rate in analysis and fractionation of proteins with molecular weights of about 10,000 to 100,000.

Applications

Comparison of Retention of each Packing Agent with the Neutral Compounds

01234_img01.png
Column Size
Eluent
Detection
:4.6 mm × 150 mm
:CH3CN/H2O = 60/40 (v/v)
:UV 254 nm
  • Wakopak® Wakosil® 5C18, 5C8, 5C4
     Flow Rate:1.0 mL/min. at 25℃
  • Wakopak® Wakosil® 5TMS, 5Ph
     Flow Rate:0.9 mL/min. at R.T.

 

Analysis of the Protein Standards with Wakopak® Wakosil® 5C4-200

  • 01234_img02.png
  • Column
    Eluent


    Flow Rate
    Detection
    :Wakopak® Wakosil® 5C4-200 (4.6 mm × 150 mm)
    :A)CH3CN/0.1%TFA = 20/80 (v/v)
     B)CH3CN/0.1%TFA = 60/40 (v/v)
       0-20 min. B 0-100%
    :1.0 mL/min. at R.T.
    :UV 220 nm

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Wakopak® Wakosil® 5C8

C8 column for reverse-phase chromatography

Wakopak® Wakosil® 5C4

C4 column for reverse-phase chromatography

Wakopak® Wakosil® 5C4-200

C4 column for reverse-phase chromatography

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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