HPLC Column for Combinatorial Chemistry "Wakopak® Combi Series"

Wakopak® Combi Series

Wakopak® Combi series are HPLC columns that can be applied to a variety of compounds and enable rapid analysis, separation, and purification, which are required in combinatorial chemistry.

Features

  • High separation/high retention properties even in mobile phases of high-water content
  • A high-end capping packing agent which suitable for basic compound analysis is used
  • Effective for high-flow rate gradient method due to low-column pressure
  • Easy to scale up from analysis to fractionation

Wakopak® Combi ODS, Wakopak® Combi ODS fast

Wakopak® Combi ODS series adopts high-purity ODS (5 μm, particle size). They can be applied to a wide-range of compounds, including hydrophilic/hydrophobic compounds and metal-coordination compounds.

Wakopak® Combi ODS series are designed to minimize pressure loss to support gradient methods that enable separation in a short time. Wakopak® Combi ODS fast series uses a packing agent with a particle size of 3 μm to achieve further reduction of column length and analysis time.

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Column :Wakopak® Combi ODS fast
 (4.6 mm x 30 mm)
Wakopak® Combi ODS
(4.6 mm x 50 mm)
Flow Rate :3.0 mL/min. 3.0 mL/min.
Gradient :0 - 2.5 min. B 0 - 80%
 2.5 - 3 min. B 80%
0 - 3.5 min. B 0 - 80%
3.5 - 4.5 min. B 80%
Eluent :A) CH3CN/H2O = 5/59(v/v), 0.05% TFA
 B) CH3CN/H2O = 95/5(v/v), 0.05% TFA
Temp :R.T.
Detection :UV 280 nm 2.048AUFS
Sample :1) p-Hydroxybenzoic Acid
 2) Methyl p-hydroxybenzoate
 3) Salicylic acid
 4) Ethyl p-hydroxybenzoate
 5) Propyl p-hydroxybenzoate
 6) Methyl salicylate
7) Butyl p-hydroxybenzoate
8) Ethyl salicylate
9) Propyl salicylate
10) Butyl salicylate
 each 1 mg/mL
Injection Volume :5 µL

Wakopak® Combi CN

Wakopak® Combi CN series adopts packing agent made by introducing the cyanopropyl group to high-purity silica gel with a particle size of 5 μm, followed by end capping. Compared with ODS (C18) column, the hydrophilic compounds feature high retention, whereas the hydrophobic compounds feature low retention.

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Column :Wakopak® Combi CN
 (4.6 mm x 50 mm)
Wakopak® Combi ODS
(4.6 mm x 50 mm)
Flow Rate :2.0 mL/min. 2.0 mL/min.
Eluent :A) CH3CN/H2O = 5/59(v/v), 0.05% TFA
 B) CH3CN/H2O = 95/5(v/v), 0.05% TFA
 0 - 5.0 min. B 0 - 50%
 5.0 min. B 50%
Temp :R.T.
Detection :UV 254 nm 2.048AUFS
Sample :1) p-Hydroxybenzoic Acid
 2) Methyl p-hydroxybenzoate
 3) Ethyl p-hydroxybenzoate
 4) Propyl p-hydroxybenzoate
 5) Butyl p-hydroxybenzoate
  each 0.2 mg/mL

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

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