HPLC Column for Oligo DNA Analysis
Wakopak® Wakosil®-DNA
A dedicated column developed for the analysis and fractionation of oligonucleotides.
Efficient analysis and fractionation of synthetic ssDNA (single-stranded DNA) up to several tens of mer for each 1 mer difference. In particular, these analytical columns for oligonucleotide are said to be largely dependent on the quality of the raw material silica gel, but the Wakopak® Wakosil®-DNA uses well-selected silica gel and exhibits high separation capacity and recovery rate.
In addition, the quality is assured by the analysis test of oligo DNA for each lot of packing agent.
Analysis Examples
Analysis Example of Oligodeoxyadenosines (12 mer to 30 mer)
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Column ：Wakopak® Wakosil®-DNA (4.6 mm x 150 mm) Eluent ：A) 0.1 M TEAA* (pH7.0)
B) CH3CN/0.1 M TEAA (pH7.0) = 50/50 (v/v)
linear gradient 0 → 60 min. B：12 → 20%Flow Rate ：1.0 mL/min. at R.T. Detection ：UV 260 nm
Purification of Synthetic DNA (19 mer)
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①, ②
Column ：Wakopak® Wakosil®-DNA (4.6 mm x 150 mm) Eluent ：A) 0.1 M TEAA* (pH7.0)
B) CH3CN
linear gradient 0 → 30 min. B：10 → 20%Flow Rate ：1.0 mL/min. Detection ：UV 260 nm ③
Column ：Wakopak® Wakosil®-DNA (4.6 mm x 150 mm) Eluent ：A) 0.1 M TEAA* (pH7.0)
B) CH3CN
linear gradient 0 → 30 min. B：5 → 15%Flow Rate ：1.0 mL/min. Detection ：UV 260 nm
*TEAA = Triethylamine-Acetate Butter
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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