Wakopak® Wakosil®-Ⅱ 5C22

Wakopak® Wakosil®-Ⅱ 5C22 is a HPLC column packed with high-purity silica gel, which is modified with a docosyl (C22) group and end-capped. Since the C22 packing agents have a longer alkyl group and a different retention time from the octadecyl (C18) packing agents, they offer superior separation characteristics for compounds with similar structure. Wakopak® Wakosil®-II 5C22 will provide better results when analyzing samples that are difficult to separate with conventional ODS columns.

Silica Gel High-purity silica gel
Average Particle Size 5 μm
Specific Surface Area 330 m2/g
Pore Size 12.0 nm
Pore Volume 1.0 mL/g
Functional Group Docosyl group
Carbon Content 20%
USP Code -
Recommended pH Range 2.0 - 7.5


  • Unique separation behavior with C22
  • Using high-purity silica gel
  • Higher resolution, higher number of theoretical plates
  • Available with various column sizes (Inner diameter of Φ2.0 to Φ6.0)


Analysis Example of 8 Type Organic Compounds

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  • HPLC Conditions
    Column : Wakopak® Wakosil®-Ⅱ 5C22 4.6 mm x 250 mm
    Eluent : CH3OH/H2O=50/50 (v/v)
    Flow Rate : 1.0 mL/min. at 40℃
    Detection : UV 254 nm, 0.128 AUFS
    Sample 1. Uracil
    2. Caffeine
    3. Phenol
    4. 2-Ethylpyridine
    5. Methyl Benzoate
    6. Benzene
    7. N,N-Dimethylaniline
    8. Toluene

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Wakopak® Wakosil®-Ⅱ 5C22

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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