Silica Gel Packing Agent for HPLC Columns (Unmodified, C18 Modified)

Silica gel has a strong physical strength and is the most widely used as a column packing agent for analysis and purification in chromatographies such as HPLC, medium and low pressure chromatography, and open column chromatography.

In general, there are unmodified silica gel and chemically modified silica gel that has been changed their functions by binding of various functional groups (C18, NH2 etc.) to the silanol groups on the surface of silica gel.

FUJIFILM Wako provides lineups of spherical silica gel (Wakosil® 5SIL series) that is used as a packing agent for HPLC columns used for normal-phase chromatography and spherical silica gel (Wakosil® C18 series) that is used as a packing agent for HPLC columns used for reverse-phase chromatography.

List of Physical Properties for each Silica Gel Grade

Silica gel for HPLC columns used for normal-phase chromatography (unmodified)
Product Name Shape Size Pore Size Pore Capacity Specific Area pH Intended Use Features
Wakosil® 5SIL series
Wakosil® 5SIL Spherical 4.0~6.0 μm 6 nm - 500 m²/g 1.0~4.0 Silicagel for HPLC columns used for normal-phase chromatography Silica gel for HPLC columns used for normal-phase chromatography. Product name: The number after 5SIL- = pore size.
Shape: Totally porous spherical silica gel
Use: HPLC column packing agent used for normal-phase chromatography
Wakosil® 5SIL-120 12 nm 300 m²/g
Silica gel for HPLC columns used for reverse-phase chromatography(C18)
Product Name Shape Size Pore Size Pore Capacity Specific Area pH Intended Use Features
Wakosil® C18 series
Wakosil® 5C18 Spherical 5 μm 15 nm 1.2 mL/g 300 m²/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography This is the most common C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography.
Product name: The number before C18 = particle size, -200 = Pore size 200Å (20 nm)
Shape: Spherical silica gel modified with C18 group. (ODS)
Use: HPLC columns packing agent for reverse-phase chromatography
Wakosil® 10C18-200 Spherical 10 μm 20 nm 1 mL/g 200 m²/g
Wakosil® C18 RS series
Wakosil®-II 3C18 RS Spherical 3.0~3.7 μm 9.5~11.5 nm 0.95~1.10 mL/g 390~430 m²/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography This series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Because its specific surface area is larger than that of Wakosil® 5C18, this series has a high retention function and separability of highly polar compounds.
Product name: The number before C18 = particle size.
Shape: Spherical silica gel modified with C18 group (ODS).
Use: HPLC columns packing agent for reverse-phase chromatography
Wakosil®-II 5C18 RS Spherical 4.2~4.7 μm 11~13 nm 1.15~1.30 mL/g 390~430 m²/g
Wakosil® C18 HG
Wakosil®-II 3C18 HG Spherical 3.2~3.7 μm 11~13 nm 0.85~1.00 mL/g 280~320 m²/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography This series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. This silica gel has the highest number of theoretical plates of the C18 series and is suitable for separating basic compounds and metal coordination compounds.
Product name: The number before C18 = particle size.
Shape: Spherical silica gel modified with C18 group (ODS)
Use: HPLC columns packing agent for reverse-phase chromatography

Wakosil® 5SIL Series

Wakosil® 5SIL series is totally porous spherical silica gel that is used as a packing agent for HPLC columns used for normal-phase adsorption chromatography.

Physical Property Information

Product Name Shape Particle Size Pore Size Specific Area pH Intended Use
Wakosil®5SIL Spherical 4.0~6.0 μm 6 nm 500 m2/g 1.0~4.0 Silica gel for HPLC columns used for normal-phase chromatography
Wakosil®5SIL-120 12 nm 300 m2/g

Wakosil® C18 Series

Wakosil® C18 series is the most common C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography.

Physical Property Information

Product Name Shape Particle Size Pore Size Pore Capacity Specific Area pH Intended Use
Wakosil®5C18 Spherical 5 μm 15 nm 1.2 mL/g 300 m2/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography
Wakosil®10C18-200 10 μm 20 nm 1 mL/g 200 m2/g

Wakosil® C18 RS Series

Wakosil® C18 RS Series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Because its specific surface area is larger than that of Wakosil® 5C18, this series has a high retention function and separability of highly polar compounds.

Physical Property Information

Product Name Shape Particle Size Pore Size Pore Capacity Specific Area pH Intended Use
Wakosil®-II 3C18 RS Spherical 3.0~3.7 μm 9.5~11.5 nm 0.95~1.10 mL/g 390~430 m²/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography
Wakosil®-II 5C18 RS 4.2~4.7 μm 11~13 nm 1.15~1.30 mL/g

Wakosil® C18 HG

Wakosil® C18 HG is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. This silica gel has the highest number of theoretical plates of the C18 series and is suitable for separating basic compounds and metal coordination compounds.

Physical Property Information

Product Name Shape Particle Size Pore Size Pore Capacity Specific Area pH Intended Use
Wakosil®-II 3C18 HG Spherical 3.2~3.7 μm 11~13 nm 0.85~1.00 mL/g 280~320 m²/g 2.0~7.5 Silica gel for HPLC columns used for reverse-phase chromatography

Product List

  • Open All
  • Close All

Wakosil® 5SIL Series

Wakosil® C18 Series

Wakosil® C18 RS Series

Wakosil® C18 HG

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

+49-2131-311-271

Reception hours: Mon-Fri 9:00 - 15:00 (CET)For other hours than the above, please contact us via the inquiry form.