C18 column

Wakopak® Wakosil®-Ⅱ 5C18, 5C18-100

Wakopak® Wakosil®- II 5C18 and 5C18-100 use high-purity silica gel with different pore sizes as the raw material.
The carbon content of Wakosil®-II 5C18-100 is as high as 22%, showing high retention.

Applications

Analysis of Aniline Derivatives

01082_img01.png
Column
Eluent
Flow Rate
Detection
:Wakopak® Wakosil®-Ⅱ 5C18 (4.6 mm × 150 mm)
:CH3OH/20 mM KH2PO4-H3PO4 buffer (pH2.7) = 5/95 (v/v)
:1.0 mL/min. at 30℃
:UV 254 nm

 

Analysis of Vitamin B1 Derivatives

01082_img02.png
Column
Eluent
Flow Rate
Detection
:Wakopak® Wakosil®-Ⅱ 5C18 (4.6 mm × 150 mm)
:7.5 mM NaH2PO4 buffer
:1.0 mL/min. at 40℃
:UV 270 nm

 

Analysis of the Active Ingredient in Scutellaria Root

01082_img03.png
Column
Eluent



Flow Rate
Detection
:Wakopak® Wakosil®-Ⅱ 5C18-100 (4.6 mm × 150 mm)
:A)0.1 M H3PO4
 B)CH3CN/0.1 M H3PO4 (v/v) = 50/50 (v/v)
    0–20 min. B conc. 40–100%
    20–30 min. B conc. 100%
:1.0 mL/min. at 50℃
:UV 280 nm 0.16 AUFS

 

Analysis of Hinokitiol in Hair Tonics

01082_img04.png
Column
Eluent

Flow Rate
Detection
:Wakopak® Wakosil®-Ⅱ 5C18-100 (4.6 mm × 150 mm)
:CH3CN/H2O containing 0.05% EDTA・2Na
 = 60/40 (v/v)
:1.0 mL/min. at RT
:UV 254 nm 0.32 AUFS

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Wakopak® Wakosil®-Ⅱ 5C18

ODS column for reverse-phase chromatography.

Wakopak® Wakosil®-Ⅱ 5C18-100

ODS column for reverse-phase chromatography.

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

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