Anti Tau Antibodies
Tau is a microtubule-associated protein predominantly expressed in the central nervous system and is a major component of the neurofibrillary tangles found in the neurons of patients with Alzheimer's disease. Because the extent of Tau accumulation in the brain correlates with the severity of cognitive impairment, Tau is considered to play a central role in the pathogenesis of Alzheimer's disease. Diseases characterized by Tau accumulation are collectively referred to as tauopathies, and the specific isoforms that accumulate differ depending on the disease. Additionally, Tau in neurofibrillary tangles is highly phosphorylated, and this phosphorylation is implicated in the pathology of Alzheimer's disease.
Fujifilm Wako offers a series of antibodies that specifically recognize total Tau, as well as individual Tau isoforms and phosphorylated Tau.
What is Tau?
Tau is a microtubule-associated protein predominantly expressed in the central nervous system, where it plays a key role in regulating microtubule stability. It is a major component of the neurofibrillary tangles found in neurons of patients with Alzheimer's disease, and Tau accumulation in the brain correlates with the severity of cognitive impairment, indicating its close involvement in the development of Alzheimer’s disease1). Neurofibrillary tangles characterized by Tau accumulation are not unique to Alzheimer's disease; they are also observed in Pick's disease, progressive supranuclear palsy, corticobasal degeneration, and other conditions, which are collectively referred to as tauopathies.
In the human central nervous system, six Tau isoforms are generated through alternative splicing. These isoforms are classified into 3-repeat Tau (3R Tau) and 4-repeat Tau (4R Tau) based on the number of microtubule-binding domains (Fig. 1). In Alzheimer's disease, neurofibrillary tangles contain both 3R Tau and 4R Tau, whereas other tauopathies show disease-specific patterns of isoform accumulation. Additionally, Tau in neurofibrillary tangles is highly phosphorylated, and this phosphorylation is implicated in the pathology of Alzheimer's disease (Fig. 2).
(Created based on Reference 2-3)
(Created based on Reference 4; green text indicates the recognition sites of anti-phosphorylated Tau antibodies available from Fujifilm Wako)
Product Lineup
Fujifilm Wako offers a series of antibodies that specifically recognize total Tau, as well as individual Tau isoforms and phosphorylated Tau.
| Recognition Tau | Clone No. | Product No. | Recognition Site |
Host | Subclass | Application | Cross-reactivity | Concentration | |
|---|---|---|---|---|---|---|---|---|---|
| Total Tau |
Human Mouse Rat |
RTM38 | 017-26893 | Tau (417-441 a.a.) |
Rat | IgG2a・κ | ICC, IHC, WB | Human, Mouse, Rat |
1.0-1.2 mg/mL |
| Human | RTM49 | 015-26953 | Tau (2-44 a.a.) |
Rat | IgG1・κ | ICC, IHC, WB | Human | 1.0-1.2 mg/mL | |
| Mouse | RTM47 | 012-26963 | Unknown | Rat | IgG2b・κ | ICC, IHC, WB | Mouse | 1.0-1.2 mg/mL | |
| Phosphorylated Tau |
T181 | 2E2-A6 | 016-26601 | Tau pT181 | Rat | IgG2b・κ | IHC, WB | Human | 1.0-1.1 mg/mL |
| S262 | TIP1-35 | 010-27123 | Tau pS262 | Rat | IgG1・λ | ELISA, ICC, WB | Human | 0.9 mg/mL | |
| S422 | AP422 | 012-27683 | Tau pS422 | Mouse | IgG1・κ | IHC, WB | Human, Mouse | 1.0 mg/mL | |
| Tau Isoform |
3R-Tau | 2A1-1F4 | 016-26581 | Tau (267-274, 306-313 a.a.) |
Rat | IgG2b・λ | IP, WB | Human | 1.0-1.1 mg/mL |
| 4R-Tau | 3E8-1A6 | 013-26591 | Tau (273-291 a.a.) |
Mouse | IgG1・κ | IP, WB | Human | 1.0-1.1 mg/mL | |
Abbreviation → ELISA: Enzyme-linked Immunosorbent Assay, ICC: Immunocytochemistry, IHC: Immunohistochemistry, IP: Immunoprecipitation, WB: Western blotting
Data
Immunohistochemistry
Anti-Total Tau Antibodies
- Species :
- Mouse (Wild-type, Tau knockout, Human Tau (P301L-tau) transgenic, Human Tau (P301L-tau) transgenic / Tau knockout)
- Site :
- Brain
- Sample :
- Paraffin section
- Antibody concentration :
- 1:500
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Anti Phosphorylated Tau T181, Rat Monoclonal Antibody (2E2-A6)
- Species :
- Human (Alzheimer’s disease)
- Site :
- Hippocampus CA1
- Sample :
- Paraffin section
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Anti Phosphorylated Tau S422, Monoclonal Antibody(AP422)
- Species :
- Human (Alzheimer’s disease)
- Site :
- Brain
Data by courtesy of Dr. Hasegawa, Department of Brain and Neurosciences, Tokyo Metropolitan Institute of Medical Science
Western Blotting
Detection of isoforms
- Sample :
- Recombinant Human Tau Mixture
- Antibody :
- 3R → Anti 3R-Tau, Rat Monoclonal Antibody(2A1-1F4)
4R → Anti 4R-Tau, Monoclonal Antibody(3E8-1A6)
Total → Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38) - Antibody concentration :
- 1:2,000
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Cross-reactivity with Tau from different animal species
- Sample :
- Recombinant Human/Mouse/Rat Tau (0N4R)
- Antibody :
- RTM38 → Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38)
RTM49 → Anti Human Tau, Rat Monoclonal Antibody(RTM49) - Antibody concentration :
- 1:2,000
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Bulk Supply of Antibody
Fujifilm Wako also offers bulk supply of this product (mg scale). Testing for antibody activity (titer) and protein concentration can also be performed upon request. For inquiries, please contact us at ffwk-labchem-tec@fujifilm.com.
Please note that, depending on your specific requirements, bulk supply may not be available, or delivery may take additional time.
Certain products may not be available for commercial use. For commercial applications, please contact Fujifilm Wako prior to such use.
References
- Nelson, P. T. et al.: J. Neuropathol. Exp. Neurol., 71(5), 362(2012).
Correlation of Alzheimer disease neuropathologic changes with cognitive status: a review of the literature - Barbier, P. et al.: Front. Aging Neurosci., 11, 204(2019).
Role of tau as a microtubule-associated protein: structural and functional aspects - Wegmann, S., Biernat, J. and Mandelkow, E.: Curr. Opin. Neurobiol., 69, 131(2021).
A current view on Tau protein phosphorylation in Alzheimer's disease
Product List
- Open All
- Close All
Anti Total Tau Antibodies
Anti phosphorylated Tau Antibodies
Anti Tau Antibodies (Isoform-specific)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.