For Alzheimer's Disease Research

Amyloid β ELISA & Amyloid β Oligomer ELISA

The early stage of Alzheimer's disease is characterized by the accumulation of insoluble senile plaques formed by the aggregation and accumulation of amyloid β (Aβ). The accumulation of senile plaque is followed by neurofibrillary changes, sheddining of nerve cells, and consequent cognitive impairment in the period of few decades. Aβ has therefore been associated with the progression of Alzheimer's disease.

Our ELISA kits enable quantification of Aβ and high-molecular Aβ oligomers which are the aggregates of Aβ.

Features

  • Can detect Aβ40 and 42 derived not only from tissue homogenates, cell culture supernates, cerebrospinal fluid (CSF), but also from plasma which is considered as hard to detect.
  • Can detect high molecular Aβ oligomer (≧ 9 mer)
  • Highly specific monoclonal antibody, developed by Takeda Pharmaceutical Company, Ltd..
  • The antibodies used in the kits are commercially available. → For more information.
  • Abundant references → See references list

Principle of the assay

01146_imgR2.png

Kit variation

β Amyloid ELISA Kit Wako To reduce unspecific bindings, using Fab' fragment antibody.
β Amyloid ELISA Kit Wako Ⅱ To increase the stability of antigen-antibody reaction, using F(ab')2 fragment antibody.
β Amyloid ELISA Kit Wako, High Sensitive The sensitivity is higher about ten times than the conventional products. Lower unspecific bindings by using Fab' fragment antibody.

Selection Guide of Aβ-related ELISA kits

Wako Cat. No. Product Name Clone No. of the antibodies Detectable Aβ Measuring Range (pmol/L)
Capture Detection Human
A
(1-40)
Human

(1-42)
Rat
(Mouse)
Aβ(1-40)
Rat
(Mouse)
Aβ(1-42)

(x-40)
*1

(x-40)
*1
Human Aβ
Oligomer
(≦8 mer)
Human Aβ
Oligomer
(≦9 mer)
292-62301 Human βAmyloid(1-40)
ELISA Kit
BAN50 BA27(Fab’) + - - - - - *4 *4 1.0 - 100
298-64601 Human βAmyloid(1-40)
ELISA Kit Ⅱ*2
BAN50 BA27(F[ab’]2) + - - - - - *4 *4 1.0 - 100
298-62401 Human βAmyloid(1-42)
ELISA Kit
BAN50 BC05(Fab’) - + - - - - *4 *4 1.0 - 100
296-64401 Human βAmyloid(1-42)
ELISA Kit, High-Sensitive
BAN50 BC05(Fab’) - + - - - - *4 *4 0.1- 20
294-62501 Human/Rat βAmyloid(40)
ELISA Kit
BNT77 BA27(Fab’) + - + - + - *4 *4 1.0 - 100
294-64701 Human/Rat βAmyloid(40)
ELISA Kit Ⅱ*3
BNT77 BA27(F[ab’]2) + - + - + - *4 *4 1.0 - 100
290-62601 Human/Rat βAmyloid(42)
ELISA Kit
BNT77 BC05(Fab’) - + - + - + *4 *4 1.0 - 100
292-64501 Human/Rat βAmyloid(42)
ELISA Kit, High-Sensitive
BNT77 BC05(Fab’) - + - + - + *4 *4 1.0 - 20
298-80101 High Molecular Amyloid β
Oligomer ELISA Kit
BAN50 BAN50 - - - - - - - + 0.41 - 100*5

*1 Aβ(x-40) and Aβ(x-42) are Aβ peptides modified/ cleaved at N-terminal.
*2 Superior antigen-antibody reaction stability compared with 292-62301
*3 Superior antigen-antibody reaction stability compared with 294-62501
*4 Not tested.
*5 Calculated on the basis of 16-mer MAP peptide

Sample type

Wako Cat. No. Product Name Cerebrospinal fluid Brain tissue extract Serum Plasma Cell culture supernatant
292-62301 Human βAmyloid(1-40)
ELISA Kit
+ + + + +
298-64601 Human βAmyloid(1-40)
ELISA Kit Ⅱ*2
+ + + + +
298-62401 Human βAmyloid(1-42)
ELISA Kit
+ + + *6 + +
296-64401 Human βAmyloid(1-42)
ELISA Kit, High-Sensitive
+ + + *6 + +
294-62501 Human/Rat βAmyloid(40)
ELISA Kit
+ + + + +
294-64701 Human/Rat βAmyloid(40)
ELISA Kit Ⅱ*3
+ + + + +
290-62601 Human/Rat βAmyloid(42)
ELISA Kit
+ + + *6 + +
292-64501 Human/Rat βAmyloid(42)
ELISA Kit, High-Sensitive
+ + + *6 + +
298-80101 High Molecular Amyloid β Oligomer
ELISA Kit
+ + - - *4

*2 Superior antigen-antibody reaction stability compared with 292-62301
*3 Superior antigen-antibody reaction stability compared with 294-62501
*4 Not tested.
*5 Calculated on the basis of 16-mer MAP peptide
*6 Detectable, but the measured value tends to decrease over time compared to plasma samples.

Product List

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ELISA Kits

Immunohistochemical Staining Kits

Related product list

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anti-Aβ / anti-APP Antibodies

Aβ / Aβ-related Proteins

Product content may differ from the actual image due to minor specification changes etc.

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