Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Source :
- Mouse
- Cross‒Reactivity :
- Mouse, Rat, Human
- Host :
- Rat
- Clone No. :
- RTM38
- Classification :
- Monoclonal
- Application :
- WB(1:1,000-2,000), ICC(1:500), IHC(1:200)
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50uL
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Product Overview
Tau is a microtubule-associated protein predominantly expressed in the central nervous system and is a major component of the neurofibrillary tangles found in the neurons of patients with Alzheimer's disease. Because the extent of Tau accumulation in the brain correlates with the severity of cognitive impairment, Tau is considered to play a central role in the pathogenesis of Alzheimer's disease. Diseases characterized by Tau accumulation are collectively referred to as tauopathies, and the specific isoforms that accumulate differ depending on the disease. Additionally, Tau in neurofibrillary tangles is highly phosphorylated, and this phosphorylation is implicated in the pathology of Alzheimer's disease.
“Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38)” is a rat monoclonal antibody that recognizes total tau in humans, mice, and rats. It can be used for immunocytochemistry, immunohistochemistry and Western blotting.
Antibody Information
| Clonality | Monoclonal (Clone No. RTM38) |
|---|---|
| Antigen | Recombinant mouse Tau (0N4R, Full-length) |
| Host | Rat |
| Formulation | PBS, 50% Glycerol, 0.05% Sodium azide |
| Conjugate | Unconjugated |
| Cross-reactivity | Human, Mouse, Rat |
| Application | Immunocytochemistry 1:500 Immunohistochemistry (Frozen/Paraffin/Vibratome section) 1:200 Western blotting 1:1,000-2,000 |
Data
Performance Data
Detection of isoforms
- Sample:
- Recombinant Human Tau Mixture
- Antibody:
- 3R → Anti 3R-Tau, Rat Monoclonal Antibody(2A1-1F4)
4R → Anti 4R-Tau, Monoclonal Antibody(3E8-1A6)
Total → Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38)
- Antibody concentration:
- 1:2,000
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Cross-reactivity with Tau from different animal species
- Sample:
- Recombinant Human/Mouse/Rat Tau (0N4R)
- Antibody:
- RTM38 → Anti Human/Mouse/Rat Tau, Rat Monoclonal Antibody(RTM38)
RTM49 → Anti Human Tau, Rat Monoclonal Antibody(RTM49)
- Antibody concentration:
- 1:2,000
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
Application Data
Immunohistochemistry
- Species:
- Mouse (wild-type, Tau knockout, human Tau (P301L-tau) transgenic, Human Tau (P301L-tau) transgenic / Tau knockout)
- Site:
- Brain
- Sample:
- Paraffin section
- Antibody concentration:
- 1:500
Data by courtesy of Dr. Miyasaka, Neuropathology Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University
(affiliation at the time the data were provided)
FAQ
About Antibody
- Which region of the Tau protein does this antibody recognize?
- This antibody recognizes the C-terminal region of the Tau protein (amino acids 417 - 441).
- What is the subclass of this antibody?
- The subclass of this antibody is IgG2a·κ.
- Has this antibody been affinity purified?
- Yes, it has been affinity purified using Protein G.
- What is the concentration of this antibody?
- The specification for protein concentration is 1.0 - 1.2 mg/mL.
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human.Tau is one type of microtubule-binding protein that is primarily expressed within the neurons of the central nervous system and is involved in the modulation of microtubule stability. In Alzheimer's disease, the formation of neurofibrillary tangles with accumulated phosphorylated Tau proteins is known to occur. Since correlations between the occurrence frequency of neurofibrillary tangles and severity of dementia were reported, neurofibrillary tangles have been studied for determination of the causes and development of therapeutic agents for Alzheimer's disease. This product is a rat monoclonal antibody which recognizes Tau of human, mouse and rat. It recognizes all Tau regardless of phosphorylation modification or isoform. [Antigen] Mouse Tau recombinant [Epitope] Tau 417 - 441 a.a. [Antibody subclass] rat IgG2a kappa [Species cross reactivity] human, mouse and rat [Applications] Western blot, Immunohistochemistry, Immunocytochemistry [Reference] 1) Kubo, A., et al.: J. Comp. Neurol., 527, 985 (2019). 2) Kubo, A., et al.: J. Neurosci., 39, 34, 6781(2019). |
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Property
Antibody Information
| Antigen | Tau (aa417-441) |
|---|---|
| Antigen Alias | Tau (aa417-441) |
| Subclass | IgG2a,k |
| Labeling | Unlabeled |
Manufacturer Information
Alias
- Anti Human/Mouse/Rat Total Tau, Rat Monoclonal Antibody（Clone No. RTM38）
Anti Human/Mouse/Rat microtubule-associated protein Tau, Rat Monoclonal Antibody（Clone No. RTM38）
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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