A microglial marker

Anti Iba1 Antibody Series

Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage/microglia. Recently, microglia has attracted attentions because neurological damage effect by production of NO, TNF-α, and IL-1β have been proved in addition to its role in neurotrophic effects/neuroprotective actions. These products are antibodies that specifically recognize Iba1, and are available for a microglial marker.
In particular, "Anti Iba1, Rabbit (for immunocytochemistry) Code No. 019-19741" is used by many worldwide researchers as a standard for microglial marker antibody. There are also many references in major journals such as Nature, Cell etc.

References

Anti-Iba1, rabbit (for immunocytochemistry) product code: 019-19741

Search with google scholar with the keyword "Iba1 019-19741 Wako"

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For detailed literature information, please refer to HERE.

Journal title The number of references IF in 2017/2018
Nature 16 41.577
Cell 6 31.398
Nature Medicine 5 32.621
Nature Neuroscience 23 19.912
Nature Immunology 3 21.809
Nature Biotechnology 2 35.724
Nature Methods 2 26.919
Neuron 11 14.318
Total 68

Application 1

Application data for immunohistochemistry, rat frozen section

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Experimental condition

Code No. 019-19741/012-26723/016-26721
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/1,000

Code No. 016-26461/013-26471
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/200

These data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Application 2

Application Data –immunohistochemistry paraffin section-

016-27691:Anti Iba1, Rabbit(for Paraffin Section)

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Experimental condition

Tissue: mouse and rat hippocampus neighborhood
Section: paraffin section
Antibody concentration: 1/1,000

Application 3

Application Data -western blotting-

016-20001: Anti Iba1, Rabbit (for Western Blotting)

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Lane

  1. Iba1 protein (10 ng)
  2. Rat microglia (10 µg)
  3. Rat neuron (10 µg)
  4. Rat cortex (150 µg)
Experimental condition

SDS-PAGE: 5.5 % stacking gel, 12.5 % running gel, 100V
Blocking: 3 % skim milk in TBS at RT for 1h
Primary antibodies:anti-Iba1 antibody(1/1000) in 3 % skim milk in TTBS at 4 °C O/N
Secondary antibody: peroxidase labeled anti-rabbit antibody (1/5000) in 3 % skim milk in TTBS at RT for 1h

The data was provided by Sanagi,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Antibody Information

Product
Name
Polyclonal antibody Monoclonal antibody
Anti Iba1, Rabbit
(for Immuno- cytochemistry)
Anti Iba1, Rabbit
(for Paraffin Section)
Anti Iba1, Rabbit
Biotin- conjugated
Anti Iba1, Rabbit
Red Fluorochrome (635)- conjugated
Anti Iba1, Rabbit
(for Western Blotting)
Anti Iba1, Monoclonal
Antibody (NCNP24)
Anti Human Iba1, Monoclonal
Antibody (NCNP27)
Cat Code
(Pkg. Size)
019-19741
(50 µg)
013-27691
(50 µg)
016-26461
(100 µL)
013-26471
(100 µL)
016-20001
(50 µg)
012-26723
(10 µL)
016-26721
(50 µL)
017-27591
(10 µL)
013-27593
(50 µL)
Antigen Synthetic peptide
(C-terminal of Iba1)
Synthetic peptide
(C-terminal of Iba1)
Clone No. -
(Polyclonal)
NCNP24 NCNP27
Subclass Rabbit IgG Mouse IgG1 Mouse IgG2
Conjugate - - Biotin Red Fluorochrome
(Ex=634, Em=654 nm)
- - -
Concentration 0.5 mg/mL 0.5 mg/mL 0.5 mg/mL 0.5 mg/mL 0.5 mg/mL 1.3 mg/mL
(First lot)
1.0 mg/mL
(First lot)
Buffer TBS TBS PBS, 0.05% NaN3 PBS, 0.05% NaN3 TBS 50% Glycerol / TBS, 0.05% NaN3 50% Glycerol / TBS, 0.05% NaN3
Cross-
reactivity
Mouse, rat, human,etc Mouse, rat Mouse, rat Mouse, rat Mouse, rat, human Mouse, rat, marmoset Human
Applications ■Immunohisto-
chemistry
(frozen section)
1:500-1,000

■Immunohisto-
chemistry
1:500-1,000
■Immunohisto-
chemistry
(paraffin section)
1:500-1,000
■Immunohistochemistry (frozen section)
1:200-2,000
■Western Blotting
1:500-1,000
■Immuno-
histochemistry
(frozen section, DAB)
1:500-2,000

■Immuno-
histochemistry
(frozen section, fluorescent)
1:100
■Immuno-
histochemistry
(paraffin section,
DAB)
1:100-1,000
Reference 1)2)3)4)5) - 6) - 7)8) 9)10) -
  • The paper using samples of dog1), cat2), pig3), marmoset4), and zebrafish5)

References
  1. Ahn, J.H., et al.: Lab. Anim. Res., 28, 3, 165 (2012).
  2. Ide, T., et al.: J. Vet. Med .Sci., 72, 1, 99 (2010).
  3. Gaige, S., et al.: Neurotoxicology., 34, 135(2013).
  4. Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
  5. Fantin, A., et al.: Blood, 116, 5, 829 (2010).
  6. Jones, M. E., et al.: Brain Behav. Immun., 67, 355(2018).
  7. Sun, J.S. et al.: Mol. Med. Rep., 12, 2, 2677(2015).
  8. Grishchuk, Y., et al.: Am. J. Pathol., 186, 1, 199(2016).
  9. Wan, S., et al.: J. Neuroinflammation, 15, 31(2018).
  10. Chen, Y., et al.: Annals of Clinical and Translational Neurology,10.1002/acn3.513 (2017).

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