A microglial marker

Microglia Marker - Iba1 Antibody Series

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Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage/microglia. Recently, microglia has attracted attentions because neurological damage effect by production of NO, TNF-α, and IL-1β have been proved in addition to its role in neurotrophic effects/neuroprotective actions. These products are antibodies that specifically recognize Iba1, and are available for a microglial marker.
In particular, "Anti Iba1, Rabbit (for immunocytochemistry) Code No. 019-19741" is used by many worldwide researchers as a standard for microglial marker antibody. There are also many references in major journals such as Nature, Cell etc.

New Product

Anti Iba1, Goat ➡ Click here for more information

SPICA Dye™ 568 Label Anti Iba1, Rabbit ➡ Click here for more information

Antibody Information

  Polyclonal Antibody Monoclonal Antibody
Product Name Most published
Anti Iba1, Rabbit
(for Immunocytochemistry)

Anti Iba1, Rabbit
(for Paraffin Section)
New
Anti, Iba1, Goat
New
Anti Iba1,Rabbit,
SPICA Dye TM 568
-conjugate

Anti Iba1, Rabbit,
Biotin
- conjugated

Anti Iba1, Rabbit
Red Fluorochrome(635)
-conjugated

Anti Iba1, Rabbit
(for Western Blotting)

Anti Iba1,
Monoclonal Antibody
(NCNP24)

Anti Human Iba1,
Monoclonal Antibody
(NCNP27)
Cat. Code No.
(Pkg. Size)
019-19741
(50 µg)
013-27691
(50 µg)
011-27991
(100 µL)
015-28011
(100μL)
016-26461
(100 µL)
013-26471
(100 µL)
016-20001
(50 µg)
012-26723
(10 µL)
016-26721
(50 µL)
017-27591
(10 µL)
013-27593
(50 µL)
Antigen Synthetic peptide
(C-terminal of Iba1)
Synthetic peptide
(C-terminal of Iba1)
Clone No. -
(Polyclonal Antibody)
NCNP24 NCNP27
Subclass -
(Polyclonal Antibody)
Mouse IgG1 Mouse IgG2b ·κ
Conjugate - - - SPICA DyeTM 568
(Ex=556nm, Em=591nm)
Biotin Red Fluorochrome
(Ex=634 nm, Em=654 nm)
- - -
Concentration 0.5-0.7 mg/mL 0.5-0.7 mg/mL 0.6-0.7 mg/mL 0.5-0.6 mg/mL 0.5-0.7 mg/mL 0.9-1.6 mg/mL 0.9-1.3 mg/mL
Buffer TBS TBS TBS PBS, 0.05% NaN3 TBS 50% Glycerol / TBS, 0.05% NaN3
Cross-reactivity Mouse, rat, human,etc Mouse, rat Mouse, rat Mouse, rat Mouse, rat, human Mouse, rat, maroset Human
Applications ■Immunohistochemistry
(frozen section)
1:500-1,000

■Immunocytochemistry
1:500-1,000
■Immunohistochemistry
(paraffin section)
1:500-1,000
■Immunohistochemistry
(frozen section)
1:200-2,000

■Immunohistochemistry
(paraffin section)
1:200-2,000

■Western blotting
1:1,000
■Immunohistochemistry
(frozen section)
1:200-2,000
■Western Blotting
1:500-1,000
■Immunohistochemistry
(frozen section, DAB)
1:500-2,000

■Immunohistochemistry
(frozen section, fluorescent)
1:500-2,000
■Immunohistochemistry
(paraffin section, DAB)
1:100-1,000
References 1)2)3)4)5) 6)7)8) 9) - 10)11)12) 13)14)15) 16)17) 18)19) -
  • The paper using samples of dog1), cat2), pig3), marmoset4), and zebrafish5)

References
  1. Ahn, J.H., et al.: Lab. Anim. Res., 28, 3, 165 (2012).
  2. Ide, T., et al.: J. Vet. Med .Sci., 72, 1, 99 (2010).
  3. Gaige, S., et al.: Neurotoxicology., 34, 135(2013).
  4. Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
  5. Fantin, A., et al.: Blood, 116, 5, 829 (2010).
  6. Harlan, BA. et al.: Exp. Neurol, 327, 113219(2020).
  7. Boucher, AA. et al.: J. Thromb. Haemost, 18, 91(2020).
  8. Harland, M., et al.: J. Neurosci, 40, 1756(2020).
  9. Ryan, KM., et al.: J. Neuroimmunol., 338, 577082(2020).
  10. Jones, M. E., et al.: Brain Behav. Immun., 67, 355(2018).
  11. Rui, Y., et al.: Nutrients, 10, 1084(2018).
  12. Noyori, O., et al.: Clin. Transl. Immunology, 8, :e1074(2019).
  13. Inyang, K. E., et al.: Pharmacol. Res., 139, 1(2019).
  14. Yamamoto, M. et al.: Cell Rep., 28, 11, 2923(2019).
  15. Nozaki, K., et al.: Neuropharmacology, 162, 107835(2020).
  16. Sun, J.S. et al.: Mol. Med. Rep., 12, 2, 2677(2015).
  17. Grishchuk, Y., et al.: Am. J. Pathol., 186, 1, 199(2016).
  18. Wan, S., et al.: J. Neuroinflammation, 15, 31(2018).
  19. Chen, Y., et al.: Annals of Clinical and Translational Neurology,10.1002/acn3.513 (2017).

Select Flowchart (immunostain)

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References

The number of publications using Wako’s Iba1 antibody per year since 2010

Searched “Iba1 019-19741 Wako” by google scholar

 

 

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Reference of major journal

Journal Title The number of references IF in 2017/2018
Nature 18 41.577
Cell 14 31.398
Nature Medicine 7 32.621
Nature Neuroscience 35 19.912
Nature Immunology 3 21.809
Nature Biotechnology 3 35.724
Nature Methods 2 26.919
Neuron 15 14.318
Total 97

Application 1

Application data for immunohistochemistry, rat frozen section

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Experimental condition

Code No. 019-19741/012-26723/016-26721
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/1,000

Code No. 016-26461/013-26471
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/200

These data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Application 2

Application Data –immunohistochemistry paraffin section-

016-27691:Anti Iba1, Rabbit(for Paraffin Section)

01213_img04.png
Experimental condition

Tissue: mouse and rat hippocampus neighborhood
Section: paraffin section
Antibody concentration: 1/1,000

Application 3

Application Data -western blotting-

016-20001: Anti Iba1, Rabbit (for Western Blotting)

01213_img03.png

Lane

  1. Iba1 protein (10 ng)
  2. Rat microglia (10 µg)
  3. Rat neuron (10 µg)
  4. Rat cortex (150 µg)
Experimental condition

SDS-PAGE: 5.5 % stacking gel, 12.5 % running gel, 100V
Blocking: 3 % skim milk in TBS at RT for 1h
Primary antibodies:anti-Iba1 antibody(1/1000) in 3 % skim milk in TTBS at 4 °C O/N
Secondary antibody: peroxidase labeled anti-rabbit antibody (1/5000) in 3 % skim milk in TTBS at RT for 1h

The data was provided by Sanagi,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

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