Anti Iba1, Rabbit Monoclonal Antibody (6A4), recombinant
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- GHS :
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20uL
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100uL
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Document
Product Overview
Iba1 (Ionized calcium-binding adapter molecule 1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system1). It is expressed in both resting and activated microglia, but is reportedly expressed more highly in activated microglia2). It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis3).
Anti Iba1, Rabbit Monoclonal Antibody (6A4), recombinant is a rabbit monoclonal antibody that has the same performance as "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741) in immunohistochemical staining of mouse and rat brains. It has also been reported to obtain good results in immunohistochemical staining of mouse retinas.
Features
- Enables even the processes of microglia to be immunohistochemical stained.
- Same performance as "Anti Iba1, Rabbit (for immunocytochemistry)".
- Relatively small amount needed, with concentrations ranging from 1:200 to 1:10000.
- Can be used for immunohistochemical staining of mouse retinas.
Antibody Information
| Clonality | Monoclonal |
|---|---|
| Antigen | Synthetic peptide (Iba1 C-terminal sequence) |
| Host | Rabbit |
| Buffer | PBS (50% Glycerol), 0.05% NaN3 |
| Concentration (mg/mL) | 1.0 - 1.2 |
| Conjugate | Unconjugated |
| Cross-reactivity | Mouse, Rat |
| Application | Immunohistochemistry (Frozen Section) 1:200 - 10,000 |
Data
Performance Data
Comparison with our conventional product (rabbit polyclonal antibody)
Polyclonal Antibody
(Product No. 019-19741)
Monoclonal Antibody
(Product No. 018-28523)
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200
Data by courtesy of
Dr.Nakajima, Faculty of Science and Engineering, Soka University
Comparison with company A (rabbit monoclonal antibody)
Company A
(rabbit, monoclonal)
Fujifilm Wako
(Product No. 018-28523)
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000
Application Data
Immunohistochemistry (Mouse)
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000
Species: Mouse
Site: Retina (Flat-mount)
Sample: Frozen section
Antibody concentration: 1:2,000
Data by courtesy of
Dr. Watanabe, Dr. Iwagawa, University of Tokyo Hospital
Normal
Activated
Species: Mouse
Site: Retina (Cross-section)
Sample: Frozen section
Antibody concentration: 1:2,000
Data by courtesy of
Dr. Watanabe, Dr. Iwagawa, University of Tokyo Hospital
Immunohistochemistry (Rat)
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000
References
- Imai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S.: Biochem. Biophys. Res. Commun., 224(3), 855(1996).
A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage - Mori, I., Imai, Y., Kohsaka, S., & Kimura, Y.: Microbiol. Immunol., 44(8), 729(2000).
Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection - Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S., & Imai, Y. Biochem. Biophys. Res. Commun., 286(2), 292(2001).
Iba1 is an actin-cross-linking protein in macrophages/microglia.
FAQ
About antibody
- What is the antigen?
- It is a synthetic peptide of Iba1 (homologous to the C-terminal sequence). The specific sequence is not disclosed.
About application
- Can this antibody be used for paraffin section?
- This antibody is not suitable for paraffin section. Please use Anti Iba1, Rabbit (for Paraffin Section) (Product Number 013-27691)
- Can this antibody be used for Western blotting?
- This antibody is suitable for immunohistochemistry (frozen sections) and immunocytochemistry. For Western blotting, use Anti Iba1, Rabbit (for Western Blotting) (Product Number 016-20001).
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human. Iba1 is a protein of about 17 kDa that is expressed specifically in microglia of the nervous system, and is frequently used as a microglial marker. This product is a rabbit monoclonal antibody that recognizes Iba1. It is for immunohistological staining with frozen section. [Antigen] C-terminal sequence peptide of Iba1 [Species cross reactivity] mouse, rat [Applications] immunohistochemical staining (frozen section) 1: 200-10,000 |
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Property
| Appearance | Liquid |
|---|---|
| Concentration | Protein : 1.0 - 1.2mg/mL |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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