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  4. ELISA / Assay Kits (Metabolism)
  5. Albumin Assay Kits
  6. LabAssay (TM) Albumin
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  2. Life Science
  3. Drug Discovery / Development
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  5. Albumin Assay Kits
  6. LabAssay (TM) Albumin
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  2. Analytical Chemistry
  3. Test for Medicinal Chemicals
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  5. Albumin Assay Kits
  6. LabAssay (TM) Albumin

LabAssay (TM) Albumin

for Cellbiology
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
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SDS
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Product Number
Package Size
Price
Inventory
Distributor
291-98901
Barcode No
4548995115263
250Tests
List Price
JPY 47,000

In stock in Japan

Please check here for notes on products and prices.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate
Analytical Charts

Kit component

For 250 tests

Chromogen Reagent 65 mL/1 bottle
Bovine Albumin Standard 1 bottle
Mouse Albumin Standard 1 bottle
Rat Albumin Standard 1 bottle

Product Overview

Albumin is a plasma protein synthesized in the liver. Serum or plasma albumin levels are important indicators in the diagnosis and management of liver disease and hepatic dysfunction. In addition, kidney diseases characterized by protein leakage into the urine are known to cause decreases in blood total protein and albumin levels, leading to hypoalbuminemia.
By measuring both albumin and total protein concentrations, the albumin-to-globulin ratio (A/G ratio) can be determined. The A/G ratio serves as a valuable marker for a range of conditions, including liver disease, kidney disease, nutritional disorders, multiple myeloma, and chronic inflammatory conditions.
LabAssay™ Albumin, a biochemical assay kit based on the bromocresol green (BCG) method, providing a simple and rapid solution for measuring albumin in blood.

[Note] LabAssay™ series are reagents for research purposes. They cannot be used for diagnostic purposes.

Kit Performance

Analysis sample *1 Human Serum/Plasma (EDTA, heparin)
Mouse Serum/Plasma (EDTA, heparin)
Rat Serum/Plasma (EDTA, heparin)
Dog Serum/Plasma (EDTA, heparin)
Cat Serum/Plasma (EDTA, heparin)
Culture medium (D-MEM, RPMI)*2
Calibration curve range 0.625-7.5 g/dL
Sample volume 2 μL
Measurement duration Approx. 20 min.
Wavelength 630 nm
*1 For animal species other than mice and rats, please use bovine albumin standards.
*2 It is tested by using the samples that standard substances have been added to the culture medium.
Measurement availability depends on the culture medium, cell type and culture conditions. Please perform a spiked recovery test or dilution linearity test in advance using the culture medium to be used in your experiment.

Example of Calibration Curve

Bovine Albumin Standard

Bovine Albumin Standard

Mouse Albumin Standard

Mouse Albumin Standard

Rat Albumin Standard

Rat Albumin Standard

Assay Principle

When the Chromogen Reagent is added to a sample, albumin binds to bromocresol green (BCG) to form a blue-colored complex. Albumin concentration is determined by measuring the intensity of the blue color.

Data

Repeatability (within run precision)

Repeatability was conducted by measuring five-fold assays of serum/plasma (EDTA) samples from human at two concentrations with this product.

n＼ID Human serum Human plasma (EDTA)
ID1 (g/dL) ID2 (g/dL) ID3 (g/dL) ID4 (g/dL)
1 4.74 6.07 4.36 6.25
2 4.84 6.30 4.52 6.46
3 4.78 5.66 4.47 6.30
4 4.79 6.09 4.54 6.26
5 4.93 5.93 4.47 6.18
mean 4.82 6.01 4.47 6.29
SD 0.0730 0.236 0.0698 0.104
CV(%) 1.5 3.9 1.6 1.7
[Note] Sample ID-2 and 4 was spiked with a standard at a concentration of 2 g/dL.

[Result]
The CV (%) of human serum was 1.5-3.9% and human plasma (EDTA) was 1.6-1.7%, indicating good repeatability.

Reproducibility (between run precision)

Reproducibility was conducted by measuring serum/plasma (EDTA) samples from human at two concentrations each for four days with this product.

Day＼ID Human serum Human plasma (EDTA)
ID9 (g/dL) ID10 (g/dL) ID11 (g/dL) ID12 (g/dL)
1 6.99 4.44 6.33 3.75
2 7.05 4.71 6.47 3.90
3 6.52 4.32 7.10 3.84
4 6.46 4.44 6.93 3.51
mean 6.76 4.48 6.71 3.75
SD 0.308 0.165 0.366 0.171
CV(%) 4.6 3.7 5.5 4.6

[Result]
The CV (%) of human serum was 3.7-4.6% and human plasma (EDTA) was 4.6-5.5%, indicating good reproducibility.

Dilution Linearity Test

Serum and plasma (EDTA) from human, mouse, rat, dog and cat as well as culture medium (D-MEM and RPMI) spiked with the standard, were serially diluted twofold with buffer in this kit and measured in duplicate to assess linearity.

Human seum
[Note] Sample ID-6 was spiked with standard at a concentration of 2 g/dL
Human plasma (EDTA)
[Note] Sample ID-8 was spiked with standard at a concentration of 2 g/dL
Mouse serum
[Note] Sample ID-1 was spiked with standard at a concentration of 5 g/dL
Mouse plasma (EDTA)
[Note] Sample ID-3 was spiked with standard at a concentration of 5 g/dL
Rat serum
[Note] Sample ID-5 was spiked with standard at a concentration of 5 g/dL
Rat plasma (EDTA)
[Note] Sample ID-7 was spiked with standard at a concentration of 5 g/dL
Dog serum
[Note] Sample ID-9 was spiked with standard at a concentration of 5 g/dL
Dog plasma (EDTA)
[Note] Sample ID-11 was spiked with standard at a concentration of 5 g/dL
Cat serum
[Note] Sample ID-13 was spiked with standard at a concentration of 5 g/dL
Cat plasma (EDTA)
[Note] Sample ID-15 was spiked with standard at a concentration of 5 g/dL
D-MEM
RPMI

[Result]
All measurement results showed good linearity within the measurement range.

Spike and Recovery Test

Standard solutions at three concentrations were added to serum/plasma (EDTA) from human, mouse, rat, dog and cat as well as culture medium (D-MEM and RPMI). Next, spike recovery tests were conducted, with each measurement performed in duplicate.

Human sample

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
Serum － 4.99 － -
1.39 6.29 1.30 93.2
2.77 7.77 2.78 100
5.54 10.3 5.31 95.8
Average 96.5
Plasma
(EDTA)		 － 4.80 － -
1.85 6.48 1.68 90.8
2.77 7.48 2.68 96.8
5.54 9.91 5.11 92.2
Average 93.3

Mouse sample (BALB/c)

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
Serum - 0.72 - -
1.40 2.25 1.53 109
2.81 3.76 3.04 108
5.62 6.42 5.70 101
Average 106
Plasma
(EDTA)		 - 0.69 - -
1.40 2.12 1.43 102
2.81 3.68 2.99 106
5.62 6.52 5.81 103
Average 104

Rat sample (S.D. rat)

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
Serum - 3.12 - -
1.40 4.40 1.28 91.4
2.81 5.77 2.65 94.3
5.62 8.54 5.42 96.4
Average 94.1
Plasma
(EDTA)		 - 2.92 - -
1.44 4.25 1.33 92.4
2.87 5.51 2.59 90.2
5.74 8.98 6.06 106
Average 96.1

Dog sample (Beagle)

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
Serum － 2.39 － -
1.90 4.18 1.79 94.2
2.85 5.07 2.68 94.0
5.69 7.93 5.54 97.4
Average 95.2
Plasma
(EDTA)		 － 2.24 － -
1.89 3.95 1.71 90.5
2.84 4.85 2.61 91.9
5.68 8.22 5.98 105
Average 95.9

Cat sample (Japanese cat)

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
Serum － 3.41 － -
1.67 4.95 1.54 92.2
2.50 5.73 2.32 92.8
5.00 8.20 4.79 95.8
Average 93.6
Plasma
(EDTA)		 － 2.31 － -
1.75 4.13 1.82 104
2.63 4.77 2.46 93.5
5.26 7.44 5.13 97.5
Average 98.4

Culture medium

Spiked amount
(g/dL)		 Measurement value
(g/dL)		 Recovery volume
(g/dL)		 Recovery rate
(%)
D-MEM - 0.00 - -
1.18 1.14 1.14 96.6
2.96 2.97 2.97 100
5.92 6.39 6.39 108
Average 102
RPMI - 0.00 - -
1.23 1.13 1.13 91.9
3.07 2.94 2.94 95.8
6.15 6.71 6.71 109
Average 98.9

[Result]
Good recovery rates were confirmed.

Correlation with Automated Analyzer Reagents

Albumin levels in serum/plasma (EDTA) samples from human were measured using LabAssay™ Albumin and reagents for automated analyzers, and the correlation between the two sets of results was evaluated.

Correlation with Automated Analyzer Reagents

[Result]
A strong correlation was confirmed between the values obtained with LabAssay™ Albumin and those obtained using reagents for automated analyzers.

Measurement Using Actual Samples

Human sample

ID＼n Measurement value (g/dL)
n=2 (Average)
Serum ID1 5.17
ID2 5.11
ID3 5.80
Plasma
(EDTA)		 ID1 4.50
ID2 5.52
ID3 5.61

Mouse sample (BALB/c)

ID＼n Measurement value
(g/dL)		 mean SD CV
(%)
1 2
Serum ID1 3.42 3.39 3.41 0.0212 0.6
ID2 3.93 3.83 3.88 0.0707 1.8
ID3 2.68 2.69 2.69 0.00707 0.3
Plasma
(EDTA)		 ID1 1.87 1.88 1.88 0.00707 0.4
ID2 2.40 2.27 2.34 0.0919 3.9
ID3 1.99 1.90 1.95 0.0636 3.3

Rat sample (S.D. rat)

ID＼n Measurement value
(g/dL)		 mean SD CV
(%)
1 2
Serum ID1 3.99 3.95 3.97 0.0283 0.7
ID2 3.83 3.75 3.79 0.0566 1.5
ID3 3.59 3.58 3.59 0.00707 0.2
Plasma
(EDTA)		 ID1 3.58 3.50 3.54 0.0566 1.6
ID2 3.43 3.55 3.49 0.0849 2.4
ID3 3.36 3.42 3.39 0.0424 1.3

Dog sample (Beagle)

ID＼n Measurement value
(g/dL)		 mean SD CV
(%)
1 2
Serum ID1 3.46 3.46 3.46 0.00 0.0
ID2 2.63 2.65 2.64 0.0141 0.5
ID3 3.02 3.20 3.11 0.127 4.1
Plasma
(EDTA)		 ID1 2.81 2.81 2.81 0.00 0.0
ID2 2.56 2.61 2.59 0.0354 1.4
ID3 2.78 2.78 2.78 0.00 0.0

Cat sample (Japanese cat)

ID＼n Measurement value
(g/dL)		 mean SD CV
(%)
1 2
Serum ID1 2.72 2.72 2.72 0.00 0.0
ID2 3.27 2.98 3.13 0.205 6.6
ID3 3.07 3.08 3.08 0.00707 0.2
Plasma
(EDTA)		 ID1 3.03 3.04 3.04 0.00707 0.2
ID2 2.21 2.19 2.20 0.0141 0.6
ID3 2.39 2.41 2.40 0.0141 0.6

Example Calculation of the Albumin-to-Globulin Ratio (A/G Ratio)

The blood A/G ratio can be determined by using LabAssay™ Albumin (this product) in conjunction with LabAssay™ Total Protein (Product No. 295-98801). The A/G ratio expresses the proportion of albumin to globulin, where globulin is defined as total protein minus albumin. The formula is shown below, along with representative calculations for human, mouse, rat, dog, and cat samples.

Human sample

Sample No. Total protein*
(g/dL)		 Albumin
(g/dL)		 A/G ratio
Serum 1 8.50 5.17 1.6
2 7.43 5.11 2.2
3 9.74 5.80 1.5
Plasma
(EDTA)		 4 7.48 4.50 1.5
5 8.52 5.52 1.8
6 8.93 5.61 1.7

Mouse sample (BALB/c)

Sample No. Total protein*
(g/dL)		 Albumin
(g/dL)		 A/G ratio
Serum 1 4.98 3.40 2.2
2 5.49 3.88 2.4
3 5.23 2.68 1.1
Plasma
(EDTA)		 4 2.96 1.88 1.7
5 4.81 2.34 1.0
6 3.13 1.95 1.6

Rat sample (S.D. rat)

Sample No. Total protein*
(g/dL)		 Albumin
(g/dL)		 A/G ratio
Serum 1 7.91 3.97 1.0
2 7.35 3.79 1.1
3 7.61 3.59 0.9
Plasma
(EDTA)		 4 6.44 3.54 1.2
5 6.51 3.49 1.2
6 6.28 3.39 1.2

Dog sample (Beagle)

Sample No. Total protein*
(g/dL)		 Albumin
(g/dL)		 A/G ratio
Serum 1 6.31 3.46 1.2
2 4.51 2.64 1.4
3 5.78 3.11 1.2
Plasma
(EDTA)		 4 4.73 2.81 1.5
5 5.01 2.59 1.1
6 7.07 2.78 0.7

Cat sample (Japanese cat)

Sample No. Total protein*
(g/dL)		 Albumin
(g/dL)		 A/G ratio
Serum 1 5.45 2.72 1.0
2 6.20 3.13 1.0
3 6.54 3.08 0.9
Plasma
(EDTA)		 4 5.96 3.03 1.0
5 5.08 2.20 0.8
6 4.41 2.40 1.2
  • Total protein was measured using our LabAssay™ Total Protein (Product No. 295-98801).

FAQ

About sample

Which anticoagulants can I use?
Anticoagulants such as heparin, citrate and EDTA do not significantly influence the assay when used in normal amounts.
What should I do with samples that exceed the measurable range?
Dilute sample with saline and repeat the assay if the measured value exceeds the measurable range, and multiply the result by the dilution factor.
Is there any influence from substances that interfere with measurement (hemolysis, chyle, ascorbic acid, bilirubin, etc.)?
Please treat the measured values of hemolyzed samples as reference values. Chyle, ascorbic acid, and bilirubin have almost no effect on the measured values. We have confirmed that there is no influence at the following concentrations.

Effects of interfering substances

Hemolysis No influence up to 250 mg/dL
Chyle No influence up to 500 FTU
Ascorbic acid No influence up to 50 mg/dL
Bilirubin-F No influence up to 5 mg/dL
Bilirubin-C No influence up to 10 mg/dL

About kit usage

What instruments, and equipment are required for the assay using this kit?
The instruments and equipment required for the use of this kit are listed below.
  • 96-well microplate (transparent type)
  • Micropipette
  • Microtube
  • Pipette
  • Plate mixer
  • Microplate reader with 630 nm wavelength filter
What is the amount of purified water to be added to the standard product?
Find and check "Reconstitution of standard" on this product page. As the amount of purified water to be added varies by lot, be sure to check it for every lot.
What are the storage conditions and shelf-life guidelines for each reagent?
Chromogen Reagent : Ready to use. After opening the bottle, store at 2°C-10°C.
Various Albumin Standards : Add the specified volume of purified water as indicated in the "Reconstitution of standard" section to the standard vial. Allow it to stand for 10 minutes, then gently mix until completely dissolved, avoiding foaming, to prepare the standard stock solution (5.00 g/dL). Thereafter, dilute with purified water at room temperature. Store any remaining standard stock solution in aliquots frozen. It is recommended to store frozen at -35°C or below. Do not repeat freeze-and-thaw cycles.

Overview / Applications

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Manufacturer Information

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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