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  4. ELISA / Assay Kits (Metabolism)
  5. Glucose Assay Kits
  6. LabAssay (TM) Glucose

LabAssay (TM) Glucose

for Cellbiology
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
Citations (1)
PubMed Central® search
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Product Number
Package Size
Price
Inventory
Distributor
291-94001
Barcode No
4548995103451
500Tests
List Price
JPY 27,000

In stock in Japan

Please check here for notes on products and prices.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate
Analytical Charts

Kit component

For 500 tests

Buffer Solution 150 mL/1 bottle
Chromogen Substrate For 150 mL/1 bottle
Standard Solution I 3 mL/1 bottle
Standard Solution II 3 mL/1 bottle

Product Overview

Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.

LabAssay™ Glucose is a kit designed to measure glucose in samples based on an enzymatic method with a combination of mutarotase and glucose oxidase.

[Note] LabAssay™ series are reagents for research purposes. They cannot be used for diagnostic purposes.

Kit Performance

Analysis sample Human Serum/Plasma
Mouse Serum/Plasma
Rat Serum/Plasma
Dog Serum/Plasma
Cat Serum/Plasma
Calibration curve range 50-500 mg/dL
Sample volume 2 μL
Measurement duration Approx. 10 min
Wavelength Primary wavelength 505 nm
Reference wavelength 600 nm

Example of Calibration Curve

Example of Calibration Curve

Assay Principle

α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase (GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

Procedure

Assay in a microplate
Perform the assay in the wells according to the following table scheme.

Test Standard Blank
Chromogen reagent 300 μL 300 μL 300 μL
Sample Sample 2µL Standard solution 2µL - *1
Mix well and incubate at 37 ℃ for 5 min.
Measure the absorbance at 505 nm*2 of the test sample and standard solution with the blank solution as the control.

*1: The addition of water is omitted because the absorbance difference in the presence of the addition of 2 μL of water does not exhibit any practical use.
*2: Use 505 nm (Primary) and 600 nm (Reference) for two wavelengths.

Data

Repeatability (within run precision)

Repeatability was conducted by measuring sixfold assays of serum samples from human, mouse and rat at two concentrations with this product.

n＼ID Human serum Mouse serum Rat serum
ID1 (mg/dL) ID2 (mg/dL) ID1 (mg/dL) ID2 (mg/dL) ID1 (mg/dL) ID2 (mg/dL)
1 320 171 372 108 424 117
2 334 164 353 104 422 115
3 326 167 379 109 415 119
4 329 167 369 110 406 112
5 328 162 359 105 413 111
6 338 171 351 107 415 114
mean 329 167 364 107 416 115
SD 6.27 3.63 11.2 2.32 6.49 3.01
CV(%) 1.9 2.2 3.1 2.2 1.6 2.6

[Result]
The CV (%) of human serum was 1.9-2.2%, mouse serum was 2.2-3.1% and rat serum was 1.6-2.6%, indicating good repeatability.

Reproducibility (between run precision)

Reproducibility was conducted by measuring serum samples from human, mouse and rat at three concentrations each for four days with this product.

Day＼ID Human serum Mouse serum Rat serum
ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL) ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL) ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL)
1 361 172 88.3 364 180 99.1 410 198 104
2 385 181 90.4 376 190 101 414 209 105
3 364 178 89.0 386 189 104 419 203 105
4 389 183 92.4 392 196 106 430 212 108
mean 375 179 90.0 380 189 103 418 206 106
SD 14.3 4.80 1.81 12.3 6.60 3.07 8.66 6.24 1.73
CV(%) 3.8 2.7 2.0 3.2 3.5 3.0 2.1 3.0 1.6

[Result]
The CV (%) of human serum was 2.0-3.8%, mouse serum was 3.0-3.5% and rat serum was 1.6-3.0%, indicating good reproducibility.

Dilution Linearity Test

Serum from human, mouse and rat spiked with the standard, were serially diluted twofold with saline and measured in duplicate to assess linearity.

Human sample
Mouse sample
Rat sample

[Result]
All measurement results showed good linearity within the measurement range.

Spike and Recovery Test

Standard solutions at four concentrations were added to serum/plasma (EDTA, heparin) from human, mouse and rat. Next, spike recovery tests were conducted, with each measurement performed in duplicate.

Human sample

Spiked amount
(mg/dL)		 Measurement value
(mg/dL)		 Recovery volume
(mg/dL)		 Recovery rate
(%)
Serum - 104 - -
109 205 101 92.7
163 246 143 87.7
218 308 204 93.6
272 363 259 95.2
Average 92.3
Plasma
(EDTA)		 - 100 - -
114 201 101 88.6
171 249 149 87.1
228 306 206 90.4
285 357 257 90.2
Average 89.1
Plasma
(heparin)		 - 103 -
112 219 116 104
168 262 159 94.6
224 308 205 91.5
280 369 266 95.0
Average 96.2

Mouse sample

Spiked amount
(mg/dL)		 Measurement value
(mg/dL)		 Recovery volume
(mg/dL)		 Recovery rate
(%)
Serum - 98.1 - -
89.2 177 78.9 98.3
134 201 103 90.6
178 251 153 88.8
223 280 182 93.8
Average 92.9
Plasma
(EDTA)		 - 93 - -
90.8 174 81.0 95.9
136 203 110 97.5
182 248 155 98.0
227 276 183 97.4
Average 97.2
Plasma
(heparin)		 - 111 -
91.2 187 76.0 83.3
137 227 116 84.7
182 263 152 83.5
228 294 183 80.3
Average 82.9

Rat sample

Spiked amount
(mg/dL)		 Measurement value
(mg/dL)		 Recovery volume
(mg/dL)		 Recovery rate
(%)
Serum - 96.1 - -
86.1 179 82.5 95.8
129 214 117 90.7
172 258 162 94.2
215 295 199 92.6
Average 93.3
Plasma
(EDTA)		 - 101 - -
95.0 193 92.0 96.8
143 226 125 87.4
190 273 172 90.5
238 310 209 87.8
Average 90.6
Plasma
(heparin)		 - 103 -
99.0 196 93.0 93.9
149 226 123 82.6
198 279 176 88.9
248 319 216 87.1
Average 88.1

[Result]
Good recovery rates were confirmed.

Measurement Using Actual Samples

Human sample

ID＼n Measurement value
(mg/dL)		 mean SD CV
(%)
1 2
Serum ID1 104 103 104 0.707 0.7
Plasma (EDTA) ID1 100 100 100 0.00 0.0

Rat sample (S.D. rat)

ID＼n Measurement value
(mg/dL)		 mean SD CV
(%)
1 2
Serum ID1 132 140 136 5.66 4.2
ID2 114 122 118 5.66 4.8
ID3 135 144 140 6.36 4.6
ID4 118 115 117 2.12 1.8
ID5 132 137 135 3.54 2.6
Plasma
(EDTA)		 ID1 107 108 108 0.707 0.7
ID2 109 112 111 2.12 1.9
ID3 124 123 124 0.707 0.6
ID4 105 111 108 4.24 3.9
ID5 110 118 114 5.66 5.0

Mouse sample (CD-1(ICR))

ID＼n Measurement value
(mg/dL)		 mean SD CV
(%)
1 2
Serum ID1 127 133 130 4.24 3.3
ID2 146 148 147 1.41 1.0
ID3 143 158 151 10.6 7.0
ID4 150 157 154 4.95 3.2
ID5 136 132 134 2.83 2.1
Plasma
(EDTA)		 ID1 86.8 87.9 87.4 0.778 0.9
ID2 88.3 94.4 91.4 4.31 4.7
ID3 100 95.1 97.6 3.46 3.6
ID4 108 98.8 103 6.51 6.3
ID5 106 109 108 2.12 2.0
Whole blood ID1 81.6 82.9 82.3 0.919 1.1
ID2 108 118 113 7.07 6.3
ID3 92.0 84.5 88.3 5.30 6.0
ID4 95.5 101 98.3 3.89 4.0
ID5 69.3 67.2 68.3 1.48 2.2

Dog sample (Beagle)

ID＼n Measurement value
(mg/dL)		 mean SD CV
(%)
1 2
Serum ID1 97.6 110 104 8.77 8.4
ID2 71.1 79.7 75.4 6.08 8.1
ID3 65.2 75.0 70.1 6.93 9.9
ID4 63.3 62.7 63.0 0.424 0.7
ID5 77.6 86.5 82.1 6.29 7.7
ID6 73.3 79.0 76.2 4.03 5.3
ID7 36.8 38.8 37.8 1.41 3.7
ID8 56.2 60.2 58.2 2.83 4.9
Plasma
（EDTA）		 ID1 97.4 89.3 93.4 5.73 6.1
ID2 96.0 90.9 93.5 3.61 3.9
ID3 88.5 81.4 85.0 5.02 5.9
ID4 78.6 71.8 75.2 4.81 6.4
ID5 85.5 80.2 82.9 3.75 4.5
ID6 75.0 71.8 73.4 2.26 3.1
ID7 85.7 83.7 84.7 1.41 1.7
ID8 83.9 82.6 83.3 0.919 1.1

Cat sample (Short hair)

ID＼n Measurement value
(mg/dL)		 mean SD CV
(%)
1 2
Serum ID1 63.2 64.7 64.0 1.06 1.7
ID2 65.2 67.1 66.2 1.34 2.0
ID3 32.4 37.0 34.7 3.25 9.4
ID4 40.2 43.3 41.8 2.19 5.3
ID5 44.8 44.2 44.5 0.424 1.0
ID6 21.4 20.3 20.9 0.778 3.7
ID7 253 248 251 3.54 1.4
ID8 98.2 91.6 94.9 4.67 4.9
Plasma
（EDTA）		 ID1 200 201 201 0.707 0.4
ID2 118 115 117 2.12 1.8
ID3 223 224 224 0.707 0.3
ID4 272 261 267 7.78 2.9
ID5 120 119 120 0.707 0.6
ID6 115 112 114 2.12 1.9
ID7 134 136 135 1.41 1.0
ID8 107 109 108 1.41 1.3

FAQ

About sample

Is there any influence from substances that interfere with measurement (ascorbic acid, bilirubin, etc.)?
Ascorbic acid and bilirubin produce a slight negative bias, while hemolysis causes a slight positive bias. However, the anticoagulants heparin, citrate, oxalate, and EDTA, as well as the glycolysis inhibitor sodium fluoride, do not affect the measured values when used at standard concentrations.

About kit usage

What instruments, and equipment are required for the assay using this kit?
The instruments and equipment required for the use of this kit are listed below.
  • 96 well microplate (transparent type)
  • Micropipette
  • Plate mixer*
  • Incubator maintaining at 37°C*
  • Microplate reader with 505 nm wavelength filter

(*This is not necessary depending on the model of the microplate reader.)

(For Test Tube method)

  • Test tube
  • Pipette
  • Incubator maintaining at 37°C
  • Spectrophotometer or colorimeter with a 505 nm wavelength filter
What are the storage conditions and shelf-life guidelines for each reagent?
Chromogen Reagent :
Dissolve 1 bottle of Chromogen Substrate with 150 mL of Buffer Solution. After reconstitution, the solution should be stored at 2°C - 10°C and used within 1 month.

Overview / Applications

Property

Manufacturer Information

Alias

  • 638-50971 (Previous Code)
    LABGLUC-M1 (Previous Code)
    298-65701 (Previous Code)
    LabAssay

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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