Albumin Quantitative Kits

LBIS Mouse/Rat Albumin ELISA Kit is a sandwich ELISA system for quantitative measurement of mouse/Rat albumin. This is intended for research use only.

Features

• Measurable in a short period (Total reaction time: 2 hours and 20 minutes)
• A minute quantity of sample is measurable (5 μL with a standard method of operating)
• Eco-friendly preservatives are used
• All reagents are solution types and are ready to use
• High measurement accuracy and reproducibility

Sample

Serum, Plasma or urine

• Heparin is recommended to be used for collection of plasma.
• Samples are diluted within the range of measurement with the accompanying buffering solution.
• Rough indications of dilution are 10,000 to 50,000 times for a serum or plasma sample and 50 to 100 times for a urine sample.

Accuracy test

Precision of assay : Mean CV<10%
Reproducibility : Mean CV<10%

Specificity

The antibodies used in this kit are specific to albumin. Cross-reactivity of the kit is shown below.

• Albumin Mouse ELISA Kit

  ※Cross reaction at 10000 ng/mL (Except FCS)

  Sample		Cross reaction
  Rat	Albumin	Less than 5%
  Human	Albumin	N.D.
  Porcine	Albumin	N.D.
  Bovine	Albumin	N.D.
  	10 % FCS	N.D.

• Albumin Rat ELISA Kit

  ※Cross reaction at 10000 ng/mL (Except FCS)

  Sample		Cross Reaction
  Mouse	Albumin	Less than 5%
  Human	Albumin	N.D.
  Porcine	Albumin	N.D.
  Bovine	Albumin	N.D.
  	10 % FCS	N.D.

Standard curve

• Mouse Albumin assay standard curve

  

• Rat Albumin assay standard curve

  

Kit Components

• 1) Anti-albumin coated plate(8 x 12)	1 plate
  2) Standard albumin mouse or rat(10 μg/mL)	1 vial
  3) Buffer solution	60 mL
  4) HRP-labeled anti-albumin antibody	100 μL
  5) TMB Solution	12 mL
  6) Stop Solution	12 mL
  7) Wash stock Solution (x 10)	100 mL
  8) Plate Seal	3 seals

• 

References

Albumin Mouse ELISA Kit

1. Mizukami, K. et al.: Naunyn. Schmiedebergs. Arch. Pharmacol., 391, 1319(2018)
2. Rhee, H. et al.: Kidney. Res. Clin. Pract., 37, 222(2018)
3. Ishikawa, H. et al.: Biosci. Biotechnol. Biochem., 82, 515(2018)

Albumin Rat ELISA Kit

1. Kanno, M. et al.: Nephrology., 23, 1046(2018)
2. Ishibashi, Y. et al.: Horm. Metab., 48, 613(2016)
3. Takeshige, Y. et al.: Hypertens., 39, 415(2016)

Features

• Measurement range Mouse/rat 6.17 to 500 μg/mL
• Samples are urine or serum
• Special antibodies for each species (monkey/mouse/rat albumin) are used for a specific reaction
• Measurable in a short period (10 minutes) with a generalpurpose biochemical autoanalyzer
• Wide measurement range with superior reproducibility
• It cannot be affected by manipulation because it is measured　with an autoanalyzer
• Parameters for each autoanalyzer made by domestic manufacturers are being prepared (except a part of them)

Accuracy test

Mouse, rat in common

Precision of assay : Mean CV<7.5%
Reproducibility : Mean CV<7.5%

Kit Components

• 1) Reagent 1 (Buffer)	18.5 mL
  2) Reagent 2 (Anti-mouse or rat albumin solution)	6.6 mL
  3) Standard mouse or rat albumin*	2.0 mL
  4) Diluent solution for standard albumin	4.0 mL

• 

*Concentration of standard mouse or rat albumin is indicated on its bottle label.

Assay procedure

For Hitachi 7180 autoanalyzer
*Note that operation of autoanalyzer is different depending on the type of machines

References

LBIS Mouse Urinary Albumin Assay Kit

1. Haku, S. et al.: Biomed. Res. Int., 2018, 2817045(2018)
2. Iwashita, Y. et al.: Am. J. Physiol. Renal. Physiol., 310, F1206(2016)
3. Zhu, K. et al.: Free. Radic. Biol. Med., 83, 21(2015)

LBIS Rat Urinary Albumin Assay Kit

1. Tatsuyori, M. et al.: Eur. J. Pharmacol., 756, 85(2015)
2. Ruixia, M. et al.: Int. J. Clin. Exp. Pathol., 8, 14063(2015)
3. Sakai, M. et al.: Eur. J. Pharmacol., 761, 109(2015)