LabAssay (TM) Phospholipid
- for Cellbiology
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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500Tests
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Document
Kit component
Kit component
| Buffer Solution | 50 mL x 3 bottles |
|---|---|
| Chromogen Substrate | for 50 mL x 3 bottles |
| Standard Solution | 5 mL x 1 bottle |
Overview
Introduction
Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the body such as emulsification and absorption of fats or coagulation of blood.
Assay principle
Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt（DAOS) with 4-aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.
Performance
| Sample | Serum, Plasma, Cell Culture Supernates |
|---|---|
| Target animals | Human, Mouse, Rat, Dog, Cat |
| Standard curve range | 75.0 ~ 596.1 mg/dL (mg/100 mL) |
| Measurement time | Approx. 10 min. |
| Amount of sample | 2 μL |
| Measurement wavelength | 600 nm(Main), 700 nm(Sub) |
Standard Curve
Procedure
| Test | Standard | Blank | |
|---|---|---|---|
| Chromogen reagent | 300 μL | 300 μL | 300 μL |
| Sample | Serum 2 μL | Standard solution 2 μL | － |
| Mix well and incubate at 37℃ for 5 min. Measure the absorbance at 600nm*2 of the test sample and standard solution with the blank solution as the control. | |||
*2: In two wavelength assay, measure using Main wavelength 600 nm/Sub wavelength 700 nm.
Data
Spike-and-Recovery Test
Spike and Recovery Test of Phospholipid, a known amount of standard is spiked into in human,mouse,rat sample n=2
| Human Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | 0.0 | 216.6 | - | - |
| 152.4 | 361.6 | 145.0 | 95.2% | |
| 204.2 | 412.4 | 195.8 | 95.9% | |
| 262.3 | 462.8 | 246.2 | 93.9% | |
| 314.2 | 521.9 | 305.3 | 97.1% | |
| Plasma (EDTA) | 0.0 | 216.5 | - | - |
| 152.4 | 359.7 | 143.2 | 94.0% | |
| 204.2 | 403.5 | 187.0 | 91.6% | |
| 262.3 | 466.6 | 250.1 | 95.3% | |
| 314.2 | 533.5 | 317.0 | 100.9% |
| Rat Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | 0.0 | 138.1 | - | - |
| 152.4 | 291.0 | 152.9 | 100.3% | |
| 204.2 | 345.1 | 207.0 | 101.4% | |
| 262.3 | 395.4 | 257.3 | 98.1% | |
| 314.2 | 459.7 | 321.6 | 102.3% | |
| Plasma (EDTA) | 0.0 | 97.8 | - | - |
| 152.4 | 246.3 | 148.5 | 97.4% | |
| 204.2 | 298.6 | 200.8 | 98.3% | |
| 262.3 | 352.3 | 254.5 | 97.0% | |
| 314.2 | 422.5 | 324.7 | 103.3% |
| Mouse Sample | Spike(mg/dL) | Mean(mg/dL) | Recovery(mg/dL) | Recovery(%) |
|---|---|---|---|---|
| Serum | 0.0 | 285.3 | - | - |
| 152.4 | 438.1 | 152.8 | 100.2% | |
| 204.2 | 486.6 | 201.3 | 98.6% | |
| 262.3 | 532.0 | 246.7 | 94.0% | |
| 314.2 | 601.3 | 316.0 | 100.6% | |
| Plasma (EDTA) | 0.0 | 111.5 | - | - |
| 152.4 | 256.5 | 145.0 | 95.1% | |
| 204.2 | 311.2 | 199.7 | 97.8% | |
| 262.3 | 368.5 | 257.0 | 98.0% | |
| 314.2 | 432.7 | 321.1 | 102.2% |
Linearity-of-Dilution Test
- Human sample
- Rat sample
- Mouse sample
Example of measurement
| Human serum, pooled, male | |||||
|---|---|---|---|---|---|
| No. | Measured(mg/dL) | Mean (mg/dL) | SD | CV(%) | |
| 1 | 240.0 | 223.9 | 231.9 | 11.4 | 4.9 |
| Human plasma K2EDTA, pooled, male | |||||
| No. | Measured(mg/dL) | Mean (mg/dL) | SD | CV(%) | |
| 1 | 211.0 | 212.6 | 211.8 | 1.10 | 0.5 |
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20% Diluted Normal Mouse serum, CD-1(ICR), male No. Measured(mg/dL) Mean (mg/dL) SD CV(%) 1 242.1 239.8 241.0 1.60 0.7 2 213.6 214.2 213.9 0.44 0.2 3 185.5 180.8 183.1 3.30 1.8 4 201.5 194.7 198.1 4.86 2.5 5 166.8 175.3 171.0 5.97 3.5 -
Normal Mouse plasma K2EDTA, CD-1(ICR), male No. Measured(mg/dL) Mean (mg/dL) SD CV(%) 1 108.2 114.5 111.4 4.45 4.0 2 124.1 121.8 122.9 1.61 1.3 3 107.0 99.7 103.4 5.15 5.0 4 120.2 109.7 115.0 7.41 6.4 5 134.4 135.2 134.8 0.58 0.4
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10% Diluted Normal Rat serum, Sprague Dawley, male No. Measured(mg/dL) Mean (mg/dL) SD CV(%) 1 137.6 131.5 134.6 4.28 3.2 2 201.3 197.3 199.3 2.88 1.4 3 167.3 169.6 168.5 1.62 1.0 4 159.7 160.2 160.0 0.33 0.2 5 166.0 161.1 163.5 3.52 2.2 -
Normal Rat plasma K2EDTA, Sprague Dawley, male No. Measured(mg/dL) Mean (mg/dL) SD CV(%) 1 99.0 96.4 97.7 1.84 1.9 2 126.9 118.7 122.8 5.82 4.7 3 109.5 106.2 107.9 2.35 2.2 4 121.3 110.7 116.0 7.50 6.5 5 129.3 113.9 121.6 10.9 8.9
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Normal Canine serum, Beagle, male No. Measured(mg/dL) Mean
(mg/dL)SD CV(%) Age(years) 1 365.7 381.0 373.3 7.64 2.0 4 2 283.8 264.4 274.1 9.74 3.6 6 3 375.1 334.2 354.6 20.49 5.8 6 4 287.5 288.0 287.8 0.26 0.1 11 5 459.9 484.8 472.3 12.47 2.6 12 6 300.2 306.3 303.3 3.06 1.0 6 7 271.8 278.4 275.1 3.34 1.2 10 8 276.4 279.3 277.8 1.46 0.5 4 -
Normal Canine plasma EDTA, Beagle, male No. Measured(mg/dL) Mean
(mg/dL)SD CV(%) Age(years) 1 340.4 316.2 328.3 12.11 3.7 4 2 321.5 345.1 333.3 11.76 3.5 6 3 351.1 374.4 362.8 11.69 3.2 10 4 322.8 327.0 324.9 2.10 0.6 12 5 337.4 364.0 350.7 13.27 3.8 6 6 303.3 316.7 310.0 6.72 2.2 11 7 318.9 321.5 320.2 1.31 0.4 10 8 310.7 306.0 308.4 2.35 0.8 12
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Normal Feline serum, Short hair, male No. Measured(mg/dL) Mean
(mg/dL)SD CV(%) Age(years) 1 244.8 246.4 245.6 0.80 0.3 3 2 193.7 188.3 191.0 2.69 1.4 3 3 236.3 240.1 238.2 1.89 0.8 6 4 229.7 229.9 229.8 0.13 0.1 4 5 226.7 227.4 227.0 0.38 0.2 6 6 239.8 236.2 238.0 1.80 0.8 6 7 305.3 299.4 302.3 2.93 1.0 1 8 159.6 157.6 158.6 0.98 0.6 1 -
Normal Feline plasma EDTA, Short hair, male No. Measured(mg/dL) Mean
(mg/dL)SD CV(%) Age(years) 1 214.5 212.0 213.3 1.24 0.6 0.5 2 114.9 116.9 115.9 0.98 0.8 1 3 199.8 194.8 197.3 2.52 1.3 3 4 180.9 184.6 182.8 1.85 1.0 0.5 5 195.6 201.5 198.5 2.94 1.5 3 6 143.7 139.3 141.5 2.21 1.6 3 7 168.2 166.3 167.2 0.92 0.5 0.5 8 183.7 178.2 181.0 2.73 1.5 1
References
- Xu, Q. et al. : Biosci. Biotechnol, Biochem., 77, 1390 (2013). Medium-Chain Fatty Acids Enhanced the Excretion of Fecal Cholesterol and Cholic Acid in C57BL/6J Mice Fed a Cholesterol-Rich Diet *Extraction liquid of mouse liver
- Tatematsu, Y. et al. : Biol. Pharma. Bull., 41, 319 (2018). Effects of the Nonsteroidal Anti-inflammatory Drug Celecoxib on Mitochondrial Function *Liposome
- Kuge, H. et al. : J. Biol. Chem., 289, 26783 (2014). Functional Compartmentalization of the Plasma Membrane of Neurons by a Unique Acyl Chain Composition of Phospholipids *Liposome
- Kessler, E. C. et al. : J. Dairy. Sci., 97, 5481 (2014). Cholesterol metabolism, transport, and hepatic regulation in dairy cows during transition and early lactation *Bovine
- Cheng, L. et al. : Transplantation, 90, 127 (2010). Role of Cholangiocyte Bile Acid Transporters in Large Bile Duct Injury After Rat Liver Transplantation *Rat
Manufacturer Information
Alias
- 639-51001 (Previous code)
LABPLIP-M1 (Previous code)
296-63801 (Previous code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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