LabAssay (TM) AST (GOT)

Aspartate aminotransferase (AST) is an aminotransferase enzyme that catalyzes the conversion of L-aspartate and α-ketoglutarate into glutamate and oxaloacetate. AST is highly abundant in the liver, myocardium, and skeletal muscle and is released into the bloodstream due to increased cell permeability or cell death. Therefore, it serves as a marker for organ damage, including liver injury. AST is also useful indicators of liver fibrosis caused by conditions such as metabolic dysfunction-associated steatotic liver disease (MASLD).
LabAssay™ AST (GOT) is a kit designed to measure AST in samples. With the use of a microplate, this kit provides a quick and convenient method for measuring AST in samples.

[Note] LabAssay™ series are reagents for research purposes. They cannot be used for diagnostic purposes.

Kit Performance

Analysis sample	Human Serum/Plasma Mouse Serum/Plasma Rat Serum/Plasma Dog Serum/Plasma Cat Serum/Plasma
Calibration curve range	0-405 U/L
Sample volume	7 μL
Measurement duration	Approx. 40 min
Wavelength	Primary wavelength 340 nm Reference wavelength 405 nm

Example of Calibration Curve

Assay Principle

When a sample is mixed with Substrate-Enzyme Solution and α-KG Solution, glutamate and oxaloacetate are formed from L-aspartate and α-ketoglutarate in a reaction catalyzed by AST in the sample. The oxaloacetate thus produced is converted to malate by malate dehydrogenase (MDH). At the same time, NADH is oxidized to NAD, and the absorbance at 340 nm decreases. Finally, the reaction is stopped using a reaction Stop Solution, and the absorbance is measured to determine the AST activity value in the sample.

Repeatability (within run precision)

Repeatability was conducted by measuring five-fold assays of serum and plasma (EDTA) samples from human at two concentrations with this product.

[Result]
The CV (%) of human serum was 1.6-2.1% and human plasma (EDTA) was 4.6-6.4%, indicating good repeatability.

Reproducibility (between run precision)

Reproducibility was conducted by measuring serum and plasma (EDTA) samples from human at three concentrations each for four days with this product.

[Result]
The CV (%) of human serum was 0.9-1.6%, and human plasma was 0.9-3.4%, indicating good reproducibility.

Dilution Linearity Test

Serum and plasma (EDTA) from human, mouse, rat, dog and cat were serially diluted twofold with saline and measured in duplicate to assess linearity.

[Result]
All measurement results showed good linearity within the measurement range.

Spike and Recovery Test

Standard solutions at three concentrations were added to serum and plasma (EDTA) from human, mouse, rat, dog and cat. Next, spike recovery tests were conducted, with each measurement performed in duplicate.

[Result]
Good recovery rates were confirmed.

Correlation with Automated Analyzer Reagents

AST levels in serum/plasma (EDTA) from human were measured using LabAssay™ AST (GOT) (this product) and reagents for automated analyzers, and the correlation between the two sets of results was evaluated.

[Result]
A strong correlation was confirmed between the values obtained with LabAssay™ AST (GOT) and those obtained using reagents for automated analyzers.