LabAssay (TM) AST (GOT)

Aspartate aminotransferase (AST) is an aminotransferase enzyme that catalyzes the conversion of L-aspartate and α-ketoglutarate into glutamate and oxaloacetate. AST is highly abundant in the liver, myocardium, and skeletal muscle and is released into the bloodstream due to increased cell permeability or cell death. Therefore, it serves as a marker for organ damage, including liver injury. AST is also useful indicators of liver fibrosis caused by conditions such as metabolic dysfunction-associated steatotic liver disease (MASLD).
LabAssay™ AST (GOT) is a kit used for the determination of AST in blood (serum, plasma). With the use of a microplate, this kit provides a quick and convenient method for measuring AST in samples.

[Note] LabAssay™ series are reagents for research purposes. It cannot be used for diagnostic purposes.

Kit Performance

Target animals	Human, Mouse, Rat, Dog, Cat
Sample	Serum, Plasma
Calibration curve range	0-405 U/L
Sample volume	7 μL
Measurement duration	Approx. 40 min
Wavelength	Primary wavelength 340 nm Reference wavelength 405 nm

Example of Calibration Curve

Principle

When a sample is mixed with Substrate-Enzyme Solution and α-KG Solution, glutamate and oxaloacetate are formed from L-aspartate and α-ketoglutarate in a reaction catalyzed by AST in the sample. The oxaloacetate thus produced is converted to malate by malate dehydrogenase (MDH). At the same time, NADH is oxidized to NAD, and the absorbance at 340 nm decreases. Finally, the reaction is stopped using a reaction Stop Solution, and the absorbance is measured to determine the AST activity value in the sample.

Precision of assay (within assay variation)

Reproducibility was confirmed by performing quintuplicate assays of serum and plasma (EDTA) samples of two concentrations from humans with this product.

[Result]
The CV (%) of human serum was 1.6-2.1% and human plasma (EDTA) was 4.6-6.4%, indicating good reproducibility.

Reproducibility (between assay variation)

Reproducibility was confirmed by measuring human serum and plasma (EDTA) samples at three concentrations each for four days with this product.

[Result]
The CV (%) of human serum was 0.89-1.6%, and human plasma was 0.90-3.4%, indicating good reproducibility.

Dilution linearity test

Serum and plasma (EDTA) from humans, mice, rats, dogs and cats were subjected to a 2-fold serial dilution with buffer in this kit and measured to confirm linearity (measured in duplicates).

[Result]
Good linearity was confirmed for all samples within the calibration curve range.

Spiked recovery test

Standard solutions at three concentrations were added to serum/plasma (EDTA) from humans, mice, rats, dogs and cats. Next, spike-recovery tests were conducted, with each measurement performed in duplicate.

[Result]
Good recovery rate was confirmed.

Correlation with the reagent for automated biochemical analyzers

LabAssay™ AST (GOT) (this product) and the reagent for automated biochemical analyzers were used to measure AST in human serum and plasma (EDTA), and the correlation was confirmed.

[Result]
A high correlation was confirmed between the measured values of LabAssay™ AST (GOT) and the reagent for automated biochemical analyzers