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  1. Top
  2. Life Science
  3. Metabolism Research
  4. ELISA / Assay Kits (Metabolism)
  5. Triglyceride Assay Kits
  6. LabAssay (TM) Triglyceride

LabAssay (TM) Triglyceride

for Cellbiology
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
Citations (5)
PubMed Central® search
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SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
291-94501
Barcode No
4548995103512
350Tests
List Price
JPY 27,000

In stock in Japan

Please check here for notes on products and prices.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate
Analytical Charts

Kit component

For 350 tests

Buffer Solution 105 mL x 1 bottle
Chromogen Substrate for 105 mL x 1 bottle
Standard Solution 4 mL x 1 bottle

Product Overview

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

LabAssay™ Triglyceride is a kit based on an enzymatic colorimetric method (GPO-DAOS method) using the Trinder reagent. With the use of the enzymes and a microplate, this kit provides a convenient method for measuring triglycerides in samples.

[Note] LabAssay™ series are reagents for research purposes. They cannot be used for diagnostic purposes.

Kit Performance

Analysis sample Human Serum/Plasma
Mouse Serum/Plasma
Rat Serum/Plasma
Dog Serum/Plasma
Cat Serum/Plasma
Calibration curve range 100-888 mg/dL
Sample volume 2 μL
Measurement duration Approx. 10 min
Wavelength Primary wavelength 600 nm
Reference wavelength 700 nm

Example of Calibration Curve

Example of Calibration Curve

Assay Principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase (GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) with 4-aminoantipyrine. LabAssay™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Procedure

Perform the assay in the wells according to the following table scheme.

Test Standard Blank
Chromogen reagent 300 μL 300 μL 300 μL
Sample Serum 2 μL Standard solution 2 μL -
Mix well and incubate at 37 ℃ for 5 min. Measure the absorbance at 600 nm*1 of the test sample and standard solution with the blank solution as the control.

*1: In two wavelength assay, measure using Primary wavelength 600 nm/Reference wavelength 700 nm.

Data

Repeatability (within run precision)

Repeatability was conducted by measuring sixfold assays of serum samples from human, mouse and rat at two concentrations with this product.

n＼ID Human serum Mouse serum Rat serum
ID1 (mg/dL) ID2 (mg/dL) ID1 (mg/dL) ID2 (mg/dL) ID1 (mg/dL) ID2 (mg/dL)
1 195 328 142 392 118 199
2 191 328 143 378 119 200
3 198 330 134 389 115 195
4 196 323 135 365 113 193
5 185 322 135 383 115 182
6 197 328 142 386 114 180
mean 194 327 139 382 116 192
SD 4.89 3.21 4.23 9.70 2.34 8.55
CV(%) 2.5 1.0 3.1 2.5 2.0 4.5

[Result]
The CV (%) of human serum was 1.0-2.5%, mouse serum was 2.5-3.1% and rat serum was 2.0-4.5%, indicating good repeatability.

Reproducibility (between run precision)

Reproducibility was conducted by measuring serum samples from human, mouse and rat at three concentrations each for four days with this product.

Day＼ID Human serum Mouse serum Rat serum
ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL) ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL) ID3 (mg/dL) ID4 (mg/dL) ID5 (mg/dL)
1 537 261 135 566 263 150 568 268 143
2 552 275 137 558 259 140 614 276 144
3 554 275 135 540 263 139 592 275 144
4 546 272 137 587 294 137 609 287 141
mean 547 271 136 563 270 142 596 277 143
SD 7.63 6.65 1.15 19.5 16.3 5.80 20.8 7.85 1.41
CV(%) 1.4 2.5 0.8 3.5 6.0 4.1 3.5 2.8 1.0

[Result]
The CV (%) of human serum was 0.8-2.5%, mouse serum was 3.5-6.0% and rat serum was 1.0-3.5%, indicating good reproducibility.

Dilution Linearity Test

Human, mouse and rat samples spiked with the standard, were serially diluted twofold with saline and measured in duplicate to assess linearity.

Human sample
Mouse sample
Rat sample

[Result]
All measurement results showed good linearity within the measurement range.

Spike and Recovery Test

Standard solutions at four concentrations were added to serum/plasma (EDTA, heparin) from human, mouse and rat. Next, spike recovery tests were conducted, with each measurement performed in duplicate.

Human sample

Spiked amount
(mmol/L)		 Measurement value
(mmol/L)		 Recovery volume
(mmol/L)		 Recovery rate
(%)
Serum - 126 - -
145 267 141 97.2
218 323 196 89.9
291 389 263 90.4
363 455 329 90.6
Average 92.0
Plasma
(EDTA)		 - 136 - -
150 276 140 93.3
225 329 193 85.8
300 414 278 92.7
374 460 324 86.6
Average 89.6
Plasma
(heparin)		 - 135 -
150 269 134 89.3
226 320 186 82.3
301 394 260 86.4
376 457 322 85.6
Average 85.9

Mouse sample

Spiked amount
(mmol/L)		 Measurement value
(mmol/L)		 Recovery volume
(mmol/L)		 Recovery rate
(%)
Serum - 133 - -
129 268 136 98.3
194 342 209 90.6
258 409 276 88.8
323 448 315 93.8
Average 92.9
Plasma
(EDTA)		 - 139 - -
129 288 149 95.9
193 342 203 97.5
258 409 270 98.0
322 446 307 97.4
Average 97.2
Plasma
(heparin)		 - 151 -
132 292 141 107
198 349 198 100
264 423 272 103
329 442 291 88.4
Average 100

Rat sample

Spiked amount
(mmol/L)		 Measurement value
(mmol/L)		 Recovery volume
(mmol/L)		 Recovery rate
(%)
Serum - 130 - -
131 257 127 96.9
196 307 177 90.3
262 370 240 91.6
327 413 283 86.5
Average 91.4
Plasma
(EDTA)		 - 128 - -
135 256 128 94.8
203 312 183 90.1
271 374 245 90.4
338 401 272 80.5
Average 89.0
Plasma
(heparin)		 - 123 -
134 257 134 100
201 305 182 90.5
268 365 242 90.3
335 400 278 83.0
Average 91.0

[Result]
Good recovery rates were confirmed.

Measurement Using Actual Samples

Human sample

ID＼n Measurement value (mmol/L) mean SD CV
(%)
1 2
Serum ID1 123 129 126 4.24 3.4
Plasma (EDTA) ID1 137 135 136 1.41 1.0

Rat sample (S.D. rat)

ID＼n Measurement value (mmol/L) mean SD CV
(%)
1 2
Serum ID1 54.5 53.1 53.8 0.990 1.8
ID2 153 153 153 0.00 0.0
ID3 105 95.8 100 6.51 6.5
ID4 120 125 123 3.54 2.9
ID5 102 107 105 3.54 3.4
Plasma
(EDTA)		 ID1 56.8 64.3 60.6 5.30 8.8
ID2 73.2 67.2 70.2 4.24 6.0
ID3 95.1 85.5 90.3 6.79 7.5
ID4 68.5 63.8 66.2 3.32 5.0
ID5 76.2 68.9 72.6 5.16 7.1

Mouse sample (CD-1(ICR))

ID＼n Measurement value (mmol/L) mean SD CV
(%)
1 2
Serum ID1 80.8 77.9 79.4 2.05 2.6
ID2 78.4 76.7 77.6 1.20 1.6
ID3 81.3 75.6 78.5 4.03 5.1
ID4 57.4 60.3 58.9 2.05 3.5
ID5 48.7 53.3 51.0 3.25 6.4
Plasma
(EDTA)		 ID1 28.8 31.7 30.3 2.05 6.8
ID2 132 145 139 9.19 6.6
ID3 62.7 68.0 65.4 3.75 5.7
ID4 29.5 25.8 27.7 2.62 9.5
ID5 58.8 55.1 57.0 2.62 4.6

Dog sample (Beagle)

ID＼n Measurement value (mmol/L) mean SD CV
(%)
1 2
Serum ID1 35.6 39.7 37.7 2.90 7.7
ID2 33.8 37.0 35.4 2.26 6.4
ID3 28.6 31.0 29.8 1.70 5.7
ID4 102 109 106 4.95 4.7
ID5 37.3 34.2 35.8 2.19 6.1
ID6 25.3 24.4 24.9 0.636 2.6
ID7 18.6 16.5 17.6 1.48 8.5
ID8 47.7 42.7 45.2 3.54 7.8
Plasma
（EDTA）		 ID1 37.2 33.9 35.6 2.33 6.6
ID2 29.0 27.6 28.3 0.990 3.5
ID3 32.5 36.0 34.3 2.47 7.2
ID4 33.5 36.4 35.0 2.05 5.9
ID5 33.2 30.3 31.8 2.05 6.5
ID6 24.9 22.9 23.9 1.41 5.9
ID7 31.9 28.4 30.2 2.47 8.2
ID8 63.2 57.0 60.1 4.38 7.3

Cat sample

ID＼n Measurement value (mmol/L) mean SD CV
(%)
1 2
Serum ID1 32.4 35.9 34.2 2.47 7.2
ID2 18.4 20.2 19.3 1.27 6.6
ID3 33.9 36.6 35.3 1.91 5.4
ID4 48.9 53.8 51.4 3.46 6.7
ID5 33.9 32.8 33.4 0.778 2.3
ID6 45.3 47.1 46.2 1.27 2.8
ID7 96.6 88.0 92.3 6.08 6.6
ID8 52.3 54.7 53.5 1.70 3.2
Plasma
（EDTA）		 ID1 104 104 104 0.00 0.0
ID2 34.3 31.8 33.1 1.77 5.3
ID3 54.8 49.3 52.1 3.89 7.5
ID4 65.1 60.7 62.9 3.11 4.9
ID5 43.9 44.4 44.2 0.354 0.8
ID6 29.0 31.9 30.5 2.05 6.7
ID7 16.8 16.2 16.5 0.424 2.6
ID8 25.0 27.2 26.1 1.56 6.0

Reference

FAQ

About sample

Is there any influence from substances that interfere with measurement (ascorbic acid, bilirubin, etc.)?
Hemolysis may cause a slight positive bias, and ascorbic acid may cause a slight negative bias. Bilirubin has little to no effect on the assay results.

About kit usage

What instruments, and equipment are required for the assay using this kit?
The instruments and equipment required for the use of this kit are listed below.
  • 96 well microplate (transparent type)
  • Micropipette
  • Plate mixer*
  • Incubator maintaining at 37°C*
  • Microplate reader with 600 nm wavelength filter

(* if the microplate reader does not have a stirring function.)

(For Test Tube method)

  • Test tube
  • Piprtte
  • Incubator maintaining at 37°C*
  • Spectrophotometer or colorimeter with 600 nm wavelength filter
Is triglyceride the only analyte measured?
The analytes measured include not only triglycerides but also diglycerides, monoglycerides, and free glycerol. These components are enzymatically converted to glycerol prior to measurement; therefore, any glycerol present in the sample is included in the measured value. Glycerol is known to be present in blood; however, its concentration is very low compared with triglycerides (4.56 ± 3.48 mg/dL; see Reference 1).
The results obtained with this product are expressed as the concentration of triolein, the most abundant triglyceride in vivo. Although the glycerol concentration in the standard solution is 31.2 mg/dL, this corresponds to 300 mg/dL when converted to triolein equivalents, based on the molecular weights of glycerol (92.10) and triolein (885.40).

Overview / Applications

Property

Manufacturer Information

Alias

  • LabAssay
    632-50991 (Previous code)
    LABTRIG-M1 (Previous code)
    290-63701 (Previous code)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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