PYROSTAR™ Neo+ | Wako LAL System

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Basics of Bacterial
Endotoxins Test
Basics of Bacterial Endotoxins Test TOP
- What is Bacterial endotoxin
Product
Information
Product Information TOP
- Endotoxin Measurement System
  Toxinometer^® ET-7000
- Endotoxin Measurement System
  Agilent Technologies, Inc. EPOCH2
- PYROSTAR™ ES-F series
  (LAL Gel-clot and Turbidimetric Reagent)
- PYROSTAR™ ES-F/Plate
  (LAL Turbidimetric Reagent)
- Limulus Color KY Series
  (LAL Chromogenic Reagent)
- Limulus ES-Ⅱ Plus CS Single Test wako
- Limulus PS Single Test wako
  (Endotoxin Detection reagent)
- PYROSTAR™ Neo+
- SLP-HS Single Reagent Set Ⅱ
- LumiMAT™ Pyrogen Detection Kit
- Pipette Tip & Microplates
- Gel-clot Reaction Tubes
- Control Standard Endotoxin
- Lysate Reagent Water
- Endotoxin Indicator Vial
- Endotoxin Extracting Solution for LAL
- ES Buffer
Service
& Support
Service & Support TOP
- FAQ
- Training & Education
About
Wako LAL system
About Wako LAL system TOP
- Bacterial Endotoxin Test and Wako
- Contribution to Measurement Technology - Acquisition of FDA approval

Recombinant Cascade Reagent (rCR) for Bacterial Endotoxin TestPYROSTAR™ Neo+

Chromogenic Technique
Endotoxin Specific
Recombinant

Catalog

Endotoxin, a major pyrogen, is commonly known as lipopolysaccharide (LPS) that is a component of the cell wall of Gram- negative bacteria. If parenteral drugs are contaminated with endotoxin, it may cause serious symptoms such as fever and shock. Bacterial Endotoxin Testing (BET) is performed using a Limulus Amebocyte Lysate (LAL) reagent made from horseshoe crab blood extracts. This is based on the phenomenon that horseshoe crab amebocyte coagulates in the presence of endotoxin. PYROSTAR™ Neo+ a horseshoe crab-free reagent for BET consists of recombinant Factor C, Factor B, and proclotting enzyme which are the main components of LAL reagent, as well as chromogenic synthetic substrate, and buffer components. Endotoxin can be measured by the chromogenic substrate method in the same way as the conventional LAL reagent.

Features
Principle
How to use
Standard curve
Experimental data
Product information

Features

➢ Less variations in reactivity between reagent lots by utilizing recombinant proteins instead ofhorseshoe crab amebocyte.
➢ Equivalent reactivity and reproducibility to conventional LAL reagents.
➢ Endotoxin-specific [No Factor G is included which is activated by (1→3)-β-D-glucan and activate pro-clotting enzyme.]
➢ Colorimetric quantification with a microplate reader or Toxinometer™ .

Principle

PYROSTAR™ Neo+ contains three recombinant proteins, horseshoe crab Factor C, Factor B, and proclotting enzyme, and chromogenic synthetic substrate t-Boc-Thr-Gly-Arg-pNA. When endotoxin is present in the sample to be tested, endotoxin activates Factor C and subsequent cascade reaction occurs as shown in the figure below, resulting in cleavage of the chromogenic substrate and release of para-nitroaniline (pNA). The amount of endotoxin in the sample is determined by the relationship between the standard endotoxin concentration and time which takes for the absorbance to reach a predetermined value, i.e., the time which takes for the amount of free pNA to reach a certain concentration.