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Bacterial Endotoxin test
Gel-clot technique, Turbidimetric technique,
Chromogenic technique

Gel-clot technique

Mix a sample with LAL reagent in a test tube and incubate it using a block heater at 37 ± 1 °C, 60 ± 2 minutes, without subjecting to vibration. Upon completion of heating, immediately but slowly tilt the tube through 180°. If a gel has formed and maintains its integrity without deformation or collapse, the result can be determined positive, while it is negative if no gel has formed. During the test, a series of samples is diluted multiple times (usually 2-fold) to check if the result is positive in each sample. The maximum valid dilution or the minimum concentration determined positive is referred to as the endpoint.

Invert the tube through about 180˚.
(Turn the tube upside down.)

Gel-clot technique

Corresponding Reagent

ES-F Series
ES-F series are Endotoxin-specific lysate, avoids false positive results from glucans.
These series are approved U.S Food and Drug Administration (FDA).
They have various gelation sensitivities, and are available in both single test and multi test kit.

Chromogenic Technique

This technique uses synthetic chromogenic substrate cleavage to detect the activation of LAL reagent induced by endotoxin.

Corresponding Reagent

Color KY Series
Color KY series can use in tube reader or microplate reader.
These series also feature measurements at the lowest concentration (highest sensitivity) among our reagent products: detection limit of 0.0002 EU/mL (single test) and 0.0005 EU/mL (multi test).

Turbidimetric Technique

This technique uses the change in gel turbidity to detect the activation of LAL reagent induced by endotoxin.

Corresponding Reagent

ES-F Series(Tube reader)
ES-F/Plate(Microplate reader)
ES-F series and ES-F/Plate are Endotoxin-specific lysate, avoids false positive results from glucans.
They are approved U.S Food and Drug Administration (FDA) .

Topics

  • LAL Basic Knowledge
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