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Endotoxin Detection ReagentsPYROSTAR™ ES-F series

  • Turbidimetric Technique
  • Gel-clot Technique
  • Endotoxin Specific
  • FDA licensed

Endotoxin-specific LAL reagents (not activated by (1→3)-β-D-glucan), compatible with the BET (USP) compliance tests U.S Food and Drug Administration (FDA) licensed. Intended Use: Limulus amebocyte lysate (LAL) is intended for the detection of gram-negative bacterial endotoxins. PYROSTAR ES-F is intended for the qualitative detection of endotoxins by gel-clot or quantitative detection by kinetic turbidimetric methods.

  • Endotoxin-specific lysate, avoids false positive results from glucans
  • Available in multi-tests vials or single-test vials
  • Can be used as either a gel-clot or Kinetic-Turbidimetric Assay (KTA) reagent
  • KTA can be performed in tube reader
  • Gel-Clot lysate sensitivities range from 0.015 to 0.25 EU/mL
  • Available with matched control standard endotoxin (CSE)
  • PYROSTAR ES-F reagents ( Gel-clot sensitivity 0.015 EU/mL ) are available with a KTA quantitative range of either 0.001 EU/mL to 10 EU/mL.
    The KTA quantitative range is related to the Gel-Clot sensitivity.
  • Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent.
  • Single test:
    The lysate reagent for one measurement is dispensed in advance in a test tube.
  • Multi test:
    The lysate reagent which dissolve in the endotoxin test water, dispense the necessary amount in reaction tubes.

PYROSTAR™ ES-F Single Test Vial

Gel-clot Sensitivity
(EU/mL)
KTA Quantitative Range
(EU/mL)
0.015 0.001 to 10
0.03 0.01 to 10
Catalog No. Product Name Quantity
540-10101 PYROSTAR™ ES-F Single Test Kit, 0.015EU/mL 25 tests
547-10351 PYROSTAR™ ES-F Single Test Kit, 0.03EU/mL 25 tests
541-10371 PYROSTAR™ ES-F Single Test Kit, 0.125EU/mL  25 tests
544-10361 PYROSTAR™ ES-F Single Test Kit, 0.25EU/mL 25 tests

CSE: 1 vial (500ng/vial)

Kit: ES-F reagent, 1 sample for 0.2 mL x 25 vials; Control Standard Endotoxin (CSE) x 1 vial

PYROSTAR ES-F Multi Test Vial with CSE

Gel-clot Sensitivity
(EU/mL)
KTA Quantitative Range
(EU/mL)
0.015 0.001 to 10
0.03 to 0.25 0.01 to 10
image6.png
2 mL
Catalog No. Manufacturer Code No. Product Name Quantity
548-10141
with CSE
WPEK4-20015 PYROSTAR ES-F MULTI KIT (2ML), 0.015 EU/ML 80 tests
541-10131
with CSE
WPEK4-20003 PYROSTAR ES-F MULTI KIT (2ML), 0.03 EU/ML 80 tests
545-10151
with CSE
WPEK4-20006 PYROSTAR ES-F MULTI KIT (2ML), 0.06 EU/ML 80 tests
542-10161
with CSE
WPEK4-20125 PYROSTAR ES-F MULTI KIT (2ML), 0.125 EU/ML 80 tests
549-10171
with CSE
WPEK4-20025 PYROSTAR ES-F MULTI KIT (2ML), 0.25 EU/ML 80 tests

CSE: 1 vial (500ng/vial)

Kit: ES-F reagent for 2 mL (20 tests with Tube reader ) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks.

5.2 mL
Catalog No. Manufacturer Code No. Product Name Quantity
543-10071
with CSE
WPEK4-50015 PYROSTAR ES-F MULTI KIT (5.2ML), 0.015 EU/ML 200 tests
542-10041
with CSE
WPEK4-50003 PYROSTAR ES-F MULTI KIT (5.2ML), 0.03 EU/ML 200 tests
547-10111
with CSE
WPEK4-50006 PYROSTAR ES-F MULTI KIT (5.2ML), 0.06 EU/ML 200 tests
544-10121
with CSE
WPEK4-50125 PYROSTAR ES-F MULTI KIT (5.2ML), 0.125 EU/ML 200 tests
549-10051
with CSE
WPEK4-50025 PYROSTAR ES-F MULTI KIT (5.2ML), 0.25 EU/ML 200 tests

CSE: 1 vial (500ng/vial)

Kit: ES-F reagent for 5.2 mL (50 tests with Tube reader ) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks

PYROSTAR ES-F Multi Test Vial without CSE (Bulk)

Gel-clot Sensitivity
(EU/mL)
KTA Quantitative Range
(EU/mL)
0.015 0.001 to 10
0.03 to 0.25 0.01 to 10
image5.jpeg
2 mL
Catalog No. Manufacturer Code No. Product Name Quantity
546-10201 WPEM-20015 PYROSTAR ES-F MULTI KIT (2ML), BULK, 0.015 EU/ML 2,000 tests
546-10181 WPEM-20003 PYROSTAR ES-F MULTI KIT (2ML), BULK, 0.03 EU/ML 2,000 tests
543-10191 WPEM-20006 PYROSTAR ES-F MULTI KIT (2ML), BULK, 0.06 EU/ML 2,000 tests
540-10221 WPEM-20125 PYROSTAR ES-F MULTI KIT (2ML), BULK, 0.125 EU/ML 2,000 tests
543-10211 WPEM-20025 PYROSTAR ES-F MULTI KIT (2ML), BULK, 0.25 EU/ML 2,000 tests

Kit: ES-F reagent for 2 mL (20 tests with Tube reader ) x 100 vials;
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks

5.2 mL
Catalog No. Manufacturer Code No. Product Name Quantity
547-10231 WPEM-50015 PYROSTAR ES-F MULTI KIT (5.2ML), BULK, 0.015 EU/ML 5,000 tests
544-10241 WPEM-50003 PYROSTAR ES-F MULTI KIT (5.2ML), BULK, 0.03 EU/ML 5,000 tests
541-10251 WPEM-50006 PYROSTAR ES-F MULTI KIT (5.2ML), BULK, 0.06 EU/ML 5,000 tests
548-10261 WPEM-50125 PYROSTAR ES-F MULTI KIT (5.2ML), BULK, 0.125 EU/ML 5,000 tests
545-10271 WPEM-50025 PYROSTAR ES-F MULTI KIT (5.2ML), BULK, 0.25 EU/ML 5,000 tests

Kit: ES-F reagent for 5.2 mL (50 tests with Tube reader ) x 100 vials;
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks

standard curve information

f1.jpg

Regression equation: log(Tg) = -0.289200 log(C) +1.13012
Correlation coefficient: -0.9984

Endotoxin concentration
(EU/mL)
Gelation time (min.)
0.001000 103.8
103.8
103.6
0.01000 49.2
49.0
48.8
0.1000 25.2
25.2
25.2
1.000 14.0
14.0
14.2

Recovery rate of spiked endotoxins with the Turbidimetric technique

Single test
f2.jpg
Multi test
f3.jpg

* Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent. Therefore, there will be a difference in sample concentration in the reaction solution. In general, single test tend to be more affected by samples.

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