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Endotoxin Detection ReagentsPYROSTAR™ ES-F series
- Turbidimetric Technique
- Gel-clot Technique
- Endotoxin Specific
- FDA licensed
Endotoxin-specific LAL reagents (not activated by (1→3)-β-D-glucan), compatible with the BET (USP) compliance tests U.S Food and Drug Administration (FDA) licensed. Intended Use: Limulus amebocyte lysate (LAL) is intended for the detection of gram-negative bacterial endotoxins. PYROSTAR™ ES-F is intended for the qualitative detection of endotoxins by gel-clot or quantitative detection by kinetic turbidimetric methods.
- Endotoxin-specific lysate, avoids false positive results from glucans
- Available in multi-tests vials or single-test vials
- Can be used as either a gel-clot or Kinetic-Turbidimetric Assay (KTA) reagent
- KTA can be performed in tube reader
- Gel-Clot lysate sensitivities range from 0.015 to 0.25 EU/mL
- Available with matched control standard endotoxin (CSE)
- PYROSTAR™ ES-F reagents ( Gel-clot sensitivity 0.015 EU/mL ) are available with a KTA quantitative range of either 0.001 EU/mL to 10 EU/mL.
The KTA quantitative range is related to the Gel-Clot sensitivity. - Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent.
- Single test:
The lysate reagent for one measurement is dispensed in advance in a test tube. - Multi test:
The lysate reagent which dissolve in the endotoxin test water, dispense the necessary amount in reaction tubes.
Technical Videos
PYROSTAR™ ES-F Single Test Vial
| Gel-clot Sensitivity (EU/mL) |
KTA Quantitative Range (EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 | 0.01 to 10 |
| Catalog No. | Product Name | Quantity |
|---|---|---|
| 540-10101 | PYROSTAR™ ES-F Single Test Kit, 0.015EU/mL | 25 tests |
| 547-10351 | PYROSTAR™ ES-F Single Test Kit, 0.03EU/mL | 25 tests |
| 541-10371 | PYROSTAR™ ES-F Single Test Kit, 0.125EU/mL | 25 tests |
| 544-10361 | PYROSTAR™ ES-F Single Test Kit, 0.25EU/mL | 25 tests |
CSE: 1 vial (500ng/vial)
Kit: ES-F reagent, 1 sample for 0.2 mL x 25 vials; Control Standard Endotoxin (CSE) x 1 vial
PYROSTAR™ ES-F Multi Test Vial with CSE
| Gel-clot Sensitivity (EU/mL) |
KTA Quantitative Range (EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 to 0.25 | 0.01 to 10 |
| Catalog No. | Manufacturer Code No. | Product Name | Quantity |
|---|---|---|---|
| 548-10141 with CSE |
WPEK4-20015 | PYROSTAR™ ES-F MULTI KIT (2ML), 0.015 EU/ML | 80 tests |
| 541-10131 with CSE |
WPEK4-20003 | PYROSTAR™ ES-F MULTI KIT (2ML), 0.03 EU/ML | 80 tests |
| 545-10151 with CSE |
WPEK4-20006 | PYROSTAR™ ES-F MULTI KIT (2ML), 0.06 EU/ML | 80 tests |
| 542-10161 with CSE |
WPEK4-20125 | PYROSTAR™ ES-F MULTI KIT (2ML), 0.125 EU/ML | 80 tests |
| 549-10171 with CSE |
WPEK4-20025 | PYROSTAR™ ES-F MULTI KIT (2ML), 0.25 EU/ML | 80 tests |
CSE: 1 vial (500ng/vial)
Kit: ES-F reagent for 2 mL (20 tests with Tube reader ) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks.
| Catalog No. | Manufacturer Code No. | Product Name | Quantity |
|---|---|---|---|
| 543-10071 with CSE |
WPEK4-50015 | PYROSTAR™ ES-F MULTI KIT (5.2ML), 0.015 EU/ML | 200 tests |
| 542-10041 with CSE |
WPEK4-50003 | PYROSTAR™ ES-F MULTI KIT (5.2ML), 0.03 EU/ML | 200 tests |
| 547-10111 with CSE |
WPEK4-50006 | PYROSTAR™ ES-F MULTI KIT (5.2ML), 0.06 EU/ML | 200 tests |
| 544-10121 with CSE |
WPEK4-50125 | PYROSTAR™ ES-F MULTI KIT (5.2ML), 0.125 EU/ML | 200 tests |
| 549-10051 with CSE |
WPEK4-50025 | PYROSTAR™ ES-F MULTI KIT (5.2ML), 0.25 EU/ML | 200 tests |
CSE: 1 vial (500ng/vial)
Kit: ES-F reagent for 5.2 mL (50 tests with Tube reader ) x 4 vials; Control Standard Endotoxin (CSE) x 1 vial
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks
PYROSTAR™ ES-F Multi Test Vial without CSE (Bulk)
| Gel-clot Sensitivity (EU/mL) |
KTA Quantitative Range (EU/mL) |
|---|---|
| 0.015 | 0.001 to 10 |
| 0.03 to 0.25 | 0.01 to 10 |
| Catalog No. | Manufacturer Code No. | Product Name | Quantity |
|---|---|---|---|
| 546-10201 | WPEM-20015 | PYROSTAR™ ES-F MULTI KIT (2ML), BULK, 0.015 EU/ML | 2,000 tests |
| 546-10181 | WPEM-20003 | PYROSTAR™ ES-F MULTI KIT (2ML), BULK, 0.03 EU/ML | 2,000 tests |
| 543-10191 | WPEM-20006 | PYROSTAR™ ES-F MULTI KIT (2ML), BULK, 0.06 EU/ML | 2,000 tests |
| 540-10221 | WPEM-20125 | PYROSTAR™ ES-F MULTI KIT (2ML), BULK, 0.125 EU/ML | 2,000 tests |
| 543-10211 | WPEM-20025 | PYROSTAR™ ES-F MULTI KIT (2ML), BULK, 0.25 EU/ML | 2,000 tests |
Kit: ES-F reagent for 2 mL (20 tests with Tube reader ) x 100 vials;
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks
| Catalog No. | Manufacturer Code No. | Product Name | Quantity |
|---|---|---|---|
| 547-10231 | WPEM-50015 | PYROSTAR™ ES-F MULTI KIT (5.2ML), BULK, 0.015 EU/ML | 5,000 tests |
| 544-10241 | WPEM-50003 | PYROSTAR™ ES-F MULTI KIT (5.2ML), BULK, 0.03 EU/ML | 5,000 tests |
| 541-10251 | WPEM-50006 | PYROSTAR™ ES-F MULTI KIT (5.2ML), BULK, 0.06 EU/ML | 5,000 tests |
| 548-10261 | WPEM-50125 | PYROSTAR™ ES-F MULTI KIT (5.2ML), BULK, 0.125 EU/ML | 5,000 tests |
| 545-10271 | WPEM-50025 | PYROSTAR™ ES-F MULTI KIT (5.2ML), BULK, 0.25 EU/ML | 5,000 tests |
Kit: ES-F reagent for 5.2 mL (50 tests with Tube reader ) x 100 vials;
LAL reagent solution can be stored below −15 ±5℃ for up to 2 weeks
standard curve information
Regression equation: log(Tg) = -0.289200 log(C) +1.13012
Correlation coefficient: -0.9984
| Endotoxin concentration (EU/mL) |
Gelation time (min.) |
|---|---|
| 0.001000 | 103.8 103.8 103.6 |
| 0.01000 | 49.2 49.0 48.8 |
| 0.1000 | 25.2 25.2 25.2 |
| 1.000 | 14.0 14.0 14.2 |
Recovery rate of spiked endotoxins with the Turbidimetric technique
Single test
Multi test
* Multi test is performed by mixing 0.1 mL of lysate reagent with 0.1 mL of sample, whereas in single test, a 0.2 mL sample directly dissolves lysate reagent. Therefore, there will be a difference in sample concentration in the reaction solution. In general, single test tend to be more affected by samples.
